Crab duplex-specific nuclease is a nuclease derived from the red king crab (Paralithodes camtschaticus, Kamchatka crab) hepatopancreas that displays a strong preference for cleaving double-stranded DNA and DNA in DNA–RNA hybrid duplexes, compared to single-stranded DNA.
The cleavage rate of short, perfectly matched DNA duplexes by this enzyme is essentially higher than that for non-perfectly matched duplexes of the same length. It has been applied to SNP detection [1] and RNA normalization. [2]
References
- ^ Shagin, DA; Rebrikov, DV; Kozhemyako, VB; Altshuler, IM; Shcheglov, AS; Zhulidov, PA; Bogdanova, EA; Staroverov, DB; Rasskazov, VA; Lukyanov, S (December 2002). "A novel method for SNP detection using a new duplex-specific nuclease from crab hepatopancreas". Genome Research. 12 (12): 1935–42. doi: 10.1101/gr.547002. PMC 187582. PMID 12466298.
- ^ Christodoulou, DC; Gorham, JM; Herman, DS; Seidman, JG (April 2011). "Construction of Normalized RNA-seq Libraries for Next-Generation Sequencing Using the Crab Duplex-Specific Nuclease". Current Protocols in Molecular Biology. Chapter 4: Unit 4.12. doi: 10.1002/0471142727.mb0412s94. PMC 3152986. PMID 21472699.
Crab duplex-specific nuclease is a nuclease derived from the red king crab (Paralithodes camtschaticus, Kamchatka crab) hepatopancreas that displays a strong preference for cleaving double-stranded DNA and DNA in DNA–RNA hybrid duplexes, compared to single-stranded DNA.
The cleavage rate of short, perfectly matched DNA duplexes by this enzyme is essentially higher than that for non-perfectly matched duplexes of the same length. It has been applied to SNP detection [1] and RNA normalization. [2]
References
- ^ Shagin, DA; Rebrikov, DV; Kozhemyako, VB; Altshuler, IM; Shcheglov, AS; Zhulidov, PA; Bogdanova, EA; Staroverov, DB; Rasskazov, VA; Lukyanov, S (December 2002). "A novel method for SNP detection using a new duplex-specific nuclease from crab hepatopancreas". Genome Research. 12 (12): 1935–42. doi: 10.1101/gr.547002. PMC 187582. PMID 12466298.
- ^ Christodoulou, DC; Gorham, JM; Herman, DS; Seidman, JG (April 2011). "Construction of Normalized RNA-seq Libraries for Next-Generation Sequencing Using the Crab Duplex-Specific Nuclease". Current Protocols in Molecular Biology. Chapter 4: Unit 4.12. doi: 10.1002/0471142727.mb0412s94. PMC 3152986. PMID 21472699.