α-Mannosidase (
EC3.2.1.24, α-D-mannosidase, p-nitrophenyl-α-mannosidase, α-D-mannopyranosidase, 1,2-α-mannosidase, 1,2-α-D-mannosidase, exo-α-mannosidase) is an
enzyme involved in the cleavage of the α form of
mannose. Its
systematic name is α-D-mannoside mannohydrolase.[3][4]
Isoenzymes
Humans express the following three α-mannosidase
isoenzymes:
It can be utilized in experiments that determine the effects of the presence or absence of mannose on specific molecules, such as recombinant proteins that are used in vaccine development.[5]
^Winchester, B. (1984). "Role of α-D-mannosidases in the biosynthesis and catabolism of glycoproteins". Biochem. Soc. Trans. 12 (3): 522–524.
doi:
10.1042/bst0120522.
PMID6428944.
^Vlahopoulos S, Gritzapis AD, Perez SA, Cacoullos N, Papamichail M, Baxevanis CN (2009). "Mannose addition by yeast Pichia Pastoris on recombinant HER-2 protein inhibits recognition by the monoclonal antibody herceptin". Vaccine. 27 (34): 4704–8.
doi:
10.1016/j.vaccine.2009.05.063.
PMID19520203.
α-Mannosidase (
EC3.2.1.24, α-D-mannosidase, p-nitrophenyl-α-mannosidase, α-D-mannopyranosidase, 1,2-α-mannosidase, 1,2-α-D-mannosidase, exo-α-mannosidase) is an
enzyme involved in the cleavage of the α form of
mannose. Its
systematic name is α-D-mannoside mannohydrolase.[3][4]
Isoenzymes
Humans express the following three α-mannosidase
isoenzymes:
It can be utilized in experiments that determine the effects of the presence or absence of mannose on specific molecules, such as recombinant proteins that are used in vaccine development.[5]
^Winchester, B. (1984). "Role of α-D-mannosidases in the biosynthesis and catabolism of glycoproteins". Biochem. Soc. Trans. 12 (3): 522–524.
doi:
10.1042/bst0120522.
PMID6428944.
^Vlahopoulos S, Gritzapis AD, Perez SA, Cacoullos N, Papamichail M, Baxevanis CN (2009). "Mannose addition by yeast Pichia Pastoris on recombinant HER-2 protein inhibits recognition by the monoclonal antibody herceptin". Vaccine. 27 (34): 4704–8.
doi:
10.1016/j.vaccine.2009.05.063.
PMID19520203.