DNA-directed RNA polymerase, mitochondrial is an
enzyme that in humans is encoded by the POLRMTgene.[5][6]
Function
This gene encodes a
mitochondrial DNA-directed
RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing
RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of
bacteriophage (including
T7 RNA polymerase), mitochondrial polymerases of lower
eukaryotes as well as chloroplastic RpoT polymerases.[6]
Structure
The structure of the enzyme has been solved. It exhibits an overall structure similar to that of phage RNAP, but the initiation mechanism is different in that it requires initiation factors
TFAM (only in mammals) and
TFB2M.[7] Elongation requires the elongation factor
TEFM.[8] The exact termination process is less understood, but
MTERF1 is thought to play a role.[9]
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4.
doi:
10.1016/0378-1119(94)90802-8.
PMID8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56.
doi:
10.1016/S0378-1119(97)00411-3.
PMID9373149.
Falkenberg M, Gaspari M, Rantanen A, et al. (2002). "Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA". Nat. Genet. 31 (3): 289–94.
doi:
10.1038/ng909.
PMID12068295.
S2CID11164308.
Overview of all the structural information available in the
PDB for
UniProt: O00411 (Human DNA-directed RNA polymerase, mitochondrial (POLRMT)) at the
PDBe-KB.
DNA-directed RNA polymerase, mitochondrial is an
enzyme that in humans is encoded by the POLRMTgene.[5][6]
Function
This gene encodes a
mitochondrial DNA-directed
RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing
RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of
bacteriophage (including
T7 RNA polymerase), mitochondrial polymerases of lower
eukaryotes as well as chloroplastic RpoT polymerases.[6]
Structure
The structure of the enzyme has been solved. It exhibits an overall structure similar to that of phage RNAP, but the initiation mechanism is different in that it requires initiation factors
TFAM (only in mammals) and
TFB2M.[7] Elongation requires the elongation factor
TEFM.[8] The exact termination process is less understood, but
MTERF1 is thought to play a role.[9]
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4.
doi:
10.1016/0378-1119(94)90802-8.
PMID8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56.
doi:
10.1016/S0378-1119(97)00411-3.
PMID9373149.
Falkenberg M, Gaspari M, Rantanen A, et al. (2002). "Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA". Nat. Genet. 31 (3): 289–94.
doi:
10.1038/ng909.
PMID12068295.
S2CID11164308.
Overview of all the structural information available in the
PDB for
UniProt: O00411 (Human DNA-directed RNA polymerase, mitochondrial (POLRMT)) at the
PDBe-KB.