DNA polymerase iota is an
enzyme that in humans is encoded by the POLIgene.[4] It is found in higher eukaryotes, and is believed to have arisen from a gene duplication from
Pol η. Pol ι, is a
Y family polymerase that is involved in
translesion synthesis. It can bypass 6-4 pyrimidine adducts and abasic sites and has a high frequency of wrong base incorporation. Like many other Y family polymerases Pol ι, has low processivity, a large DNA binding pocket and doesn't undergo conformational changes when DNA binds. These attributes are what allow Pol ι to carry out its task as a translesion polymerase. Pol ι only uses
Hoogsteen base pairing, during DNA synthesis, it will add
adenine opposite to
thymine in the syn conformation and can add both
cytosine and thymine in the anti conformation across
guanine, which it flips to the syn conformation.
Xeroderma pigmentosum variant
Xeroderma pigmentosum variant (XPV) cells lack
DNA polymerase eta (η).[5] Instead these cells use DNA polymerase iota (ι).[5] Exposure of XPV cells to UV light causes a very high frequency and unique spectrum of UV-induced mutations that can ultimately lead to malignant transformation.[5]
^
abcWang Y, Woodgate R, McManus TP, Mead S, McCormick JJ, Maher VM. Evidence that in xeroderma pigmentosum variant cells, which lack DNA polymerase eta, DNA polymerase iota causes the very high frequency and unique spectrum of UV-induced mutations. Cancer Res. 2007 Apr 1;67(7):3018-26. doi: 10.1158/0008-5472.CAN-06-3073. PMID: 17409408
Tissier A, Frank EG, McDonald JP, et al. (2001). "Biochemical characterization of human DNA polymerase iota provides clues to its biological function". Biochem. Soc. Trans. 29 (Pt 2): 183–7.
doi:
10.1042/BST0290183.
PMID11356150.
DNA polymerase iota is an
enzyme that in humans is encoded by the POLIgene.[4] It is found in higher eukaryotes, and is believed to have arisen from a gene duplication from
Pol η. Pol ι, is a
Y family polymerase that is involved in
translesion synthesis. It can bypass 6-4 pyrimidine adducts and abasic sites and has a high frequency of wrong base incorporation. Like many other Y family polymerases Pol ι, has low processivity, a large DNA binding pocket and doesn't undergo conformational changes when DNA binds. These attributes are what allow Pol ι to carry out its task as a translesion polymerase. Pol ι only uses
Hoogsteen base pairing, during DNA synthesis, it will add
adenine opposite to
thymine in the syn conformation and can add both
cytosine and thymine in the anti conformation across
guanine, which it flips to the syn conformation.
Xeroderma pigmentosum variant
Xeroderma pigmentosum variant (XPV) cells lack
DNA polymerase eta (η).[5] Instead these cells use DNA polymerase iota (ι).[5] Exposure of XPV cells to UV light causes a very high frequency and unique spectrum of UV-induced mutations that can ultimately lead to malignant transformation.[5]
^
abcWang Y, Woodgate R, McManus TP, Mead S, McCormick JJ, Maher VM. Evidence that in xeroderma pigmentosum variant cells, which lack DNA polymerase eta, DNA polymerase iota causes the very high frequency and unique spectrum of UV-induced mutations. Cancer Res. 2007 Apr 1;67(7):3018-26. doi: 10.1158/0008-5472.CAN-06-3073. PMID: 17409408
Tissier A, Frank EG, McDonald JP, et al. (2001). "Biochemical characterization of human DNA polymerase iota provides clues to its biological function". Biochem. Soc. Trans. 29 (Pt 2): 183–7.
doi:
10.1042/BST0290183.
PMID11356150.