In enzymology, a glutaryl-7-aminocephalosporanic-acid acylase ( EC 3.5.1.93) is an enzyme that catalyzes the chemical reaction

(7R)-7-(4-carboxybutanamido)cephalosporanate + H₂O ${\displaystyle \rightleftharpoons }$ (7R)-7-aminocephalosporanate + glutarate

Thus, the two substrates of this enzyme are (7R)-7-(4-carboxybutanamido)cephalosporanate and H₂O, whereas its two products are (7R)-7-aminocephalosporanate and glutarate.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is (7R)-7-(4-carboxybutanamido)cephalosporanate amidohydrolase. Other names in common use include 7beta-(4-carboxybutanamido)cephalosporanic acid acylase, cephalosporin C acylase, glutaryl-7-ACA acylase, CA, GCA, GA, cephalosporin acylase, glutaryl-7-aminocephalosporanic acid acylase, and GL-7-ACA acylase. This enzyme participates in penicillin and cephalosporin biosynthesis.

References

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• Kim Y, Yoon K, Khang Y, Turley S, Hol WG (2000). "The 2.0 A crystal structure of cephalosporin acylase". Structure. Fold. Des. 8 (10): 1059–1068. doi: 10.1016/S0969-2126(00)00505-0. PMID  11080627.
• G; Zeng, R; Ding, X; Mao, X; Ding, Y; Rao, Z; Xie, Y; Jiang, W; Zhao, G (2002). "Affinity alkylation of the Trp-B4 residue of the beta -subunit of the glutaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp 130". J. Biol. Chem. 277 (12): 10256–10264. doi: 10.1074/jbc.M108683200. PMID  11782466.
• Kim JK, Yang IS, Rhee S, Dauter Z, Lee YS, Park SS, Kim KH (2003). "Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation". Biochemistry. 42 (14): 4084–4093. doi: 10.1021/bi027181x. PMID  12680762.