In enzymology, a 7beta-hydroxysteroid dehydrogenase (NADP⁺) ( EC 1.1.1.201) is an enzyme that catalyzes the chemical reaction

a 7beta-hydroxysteroid + NADP⁺ ${\displaystyle \rightleftharpoons }$ a 7-oxosteroid + NADPH + H⁺

Thus, the two substrates of this enzyme are 7beta-hydroxysteroid and NADP⁺, whereas its 3 products are 7-oxosteroid, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is 7beta-hydroxysteroid:NADP⁺ 7-oxidoreductase. Other names in common use include NADP⁺-dependent 7beta-hydroxysteroid dehydrogenase, and 7beta-hydroxysteroid dehydrogenase (NADP⁺).

References

• Savino S, Ferrandi EE, Forneris F, Rovida S, Riva S, Monti D, Mattevi A (June 2016). "Structural and biochemical insights into 7β-hydroxysteroid dehydrogenase stereoselectivity". Proteins. 84 (6): 859–65. doi: 10.1002/prot.25036. PMID  27006087. S2CID  36310113.
• Hirano S, Masuda N (May 1982). "Characterization of NADP-dependent 7 beta-hydroxysteroid dehydrogenases from Peptostreptococcus productus and Eubacterium aerofaciens". Applied and Environmental Microbiology. 43 (5): 1057–63. Bibcode: 1982ApEnM..43.1057H. doi: 10.1128/AEM.43.5.1057-1063.1982. PMC  244185. PMID  6954878.
• MacDonald IA, Roach PD (August 1981). "Bile induction of 7 alpha- and 7 beta-hydroxysteroid dehydrogenases in Clostridium absonum". Biochimica et Biophysica Acta. 665 (2): 262–9. doi: 10.1016/0005-2760(81)90011-4. PMID  6945134.
• MacDonald IA, Rochon YP, Hutchison DM, Holdeman LV (November 1982). "Formation of ursodeoxycholic acid from chenodeoxycholic acid by a 7 beta-hydroxysteroid dehydrogenase-elaborating Eubacterium aerofaciens strain cocultured with 7 alpha-hydroxysteroid dehydrogenase-elaborating organisms". Applied and Environmental Microbiology. 44 (5): 1187–95. Bibcode: 1982ApEnM..44.1187M. doi: 10.1128/AEM.44.5.1187-1195.1982. PMC  242167. PMID  6758698.