In enzymology, a 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) ( EC 1.1.1.292) is an enzyme that catalyzes the chemical reaction

1,5-anhydro-D-mannitol + NADP⁺ ${\displaystyle \rightleftharpoons }$ 1,5-anhydro-D-fructose + NADPH + H⁺

Thus, the two substrates of this enzyme are 1,5-anhydro-D-mannitol and NADP⁺, whereas its 3 products are 1,5-anhydro-D-fructose, NADPH, and H⁺.

This enzyme belongs to the common family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is 1,5-anhydro-D-mannitol:NADP⁺ oxidoreductase. Other names in common use include 1,5-anhydro-D-fructose reductase (ambiguous), and AFR.

References

• Kuhn A, Yu S, Giffhorn F (2006). "Catabolism of 1,5-anhydro-D-fructose in Sinorhizobium morelense S-30.7.5: discovery, characterization, and overexpression of a new 1,5-anhydro-D-fructose reductase and its application in sugar analysis and rare sugar synthesis". Appl. Environ. Microbiol. 72 (2): 1248–57. doi: 10.1128/AEM.72.2.1248-1257.2006. PMC  1392929. PMID  16461673.
• Dambe TR, Kuhn AM, Brossette T, Giffhorn F, Scheidig AJ (2006). "Crystal structure of NADP(H)-dependent 1,5-anhydro-D-fructose reductase from Sinorhizobium morelense at 2.2 Å resolution: construction of an NADH-accepting mutant and its application in rare sugar synthesis". Biochemistry. 45 (33): 10030–42. doi: 10.1021/bi052589q. PMID  16906761.