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based in part on Shi [Zhengli]'s emailed answers." ( RfC, December 2021)
This section is pinned and will not be automatically archived. |
List of good sources with good coverage to help expand. Not necessarily for inclusion but just for consideration. Preferably not articles that just discuss a single quote/press conference. The long-style reporting would be even better. Feel free to edit directly to add to the list. ProcrastinatingReader ( talk) 17:39, 18 July 2021 (UTC)
Last updated by Julian Brown ( talk) 23:43, 12 November 2023 (UTC)
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For the relevant sourcing guideline, see WP:SCHOLARSHIP. For a database curated by the NCBI, see LitCoVID |
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For the relevant sourcing guideline, see WP:RSOPINION. |
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Keep in mind, these are primary sources and thus should be used with caution! |
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References
Now that the main phase of the pandemic is in the rear view window, it's clear to me that the lab-leak advocacy group DRASTIC doesn't pass WP:SUSTAINED, and could adequately be covered in a few sentences in this article. Most of the sources in that article don't even mention DRASIC, but merely debunk claims made by its members. Hemiauchenia ( talk) 02:32, 16 March 2024 (UTC)
I think it would be relevant to add the submission date for Project DEFUSE which is 3/27/18 [6]? 牢记使命 ( talk) 14:54, 2 April 2024 (UTC)
The timing of DEFUSE so close to the pandemic is obviously suspicious← that smells ripely fringey. What's the source for that? How can a abortive proposal affect a pandemic? Bon courage ( talk) 16:50, 5 April 2024 (UTC)
The current version of article contains a phrase: "There is no evidence that any genetic manipulation or reverse genetics (a technique required to make chimeric viruses) of SARS-related bat coronaviruses was ever carried out at the WIV."
It is FALSE. There is (at least one) publicly available paper which proves the contrary.
https://doi.org/10.1371/journal.ppat.1006698 is an article from 2017 with (among others) authored by Daszak and Zheng-Li Shi (the head of the WIV). "Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus".
It contains this passage:
"Construction of recombinant viruses
Recombinant viruses with the S gene of the novel bat SARSr-CoVs and the backbone of the infectious clone of SARSr-CoV WIV1 were constructed using the reverse genetic system described previously [23] (S9 Fig). The fragments E and F were re-amplified with primer pairs (FE, 5’-AGGGCCCACCTGGCACTGGTAAGAGTCATTTTGC-3’, R-EsBsaI, 5’-ACTGGTCTCTTCGTTTAGTTATTAACTAAAATATCACTAGACACC-3’) and (F-FsBsaI, 5’-TGAGGTCTCCGAACTTATGGATTTGTTTATGAG-3’, RF, 5’-AGGTAGGCCTCTAGGGCAGCTAAC-3’), respectively. The products were named as fragment Es and Fs, which leave the spike gene coding region as an independent fragment. BsaI sites (5’-GGTCTCN|NNNN-3’) were introduced into the 3’ terminal of the Es fragment and the 5’ terminal of the Fs fragment, respectively. The spike sequence of Rs4231 was amplified with the primer pair (F-Rs4231-BsmBI, 5’-AGTCGTCTCAACGAACATGTTTATTTTCTTATTCTTTCTCACTCTCAC-3’ and R-Rs4231-BsmBI, 5’-TCACGTCTCAGTTCGTTTATGTGTAATGTAATTTGACACCCTTG-3’). The S gene sequence of Rs7327 was amplified with primer pair (F-Rs7327-BsaI, 5’-AGTGGTCTCAACGAACATGAAATTGTTAGTTTTAGTTTTTGCTAC-3’ and R-Rs7327-BsaI, 5’- TCAGGTCTCAGTTCGTTTATGTGTAATGTAATTTAACACCCTTG-3’). The fragment Es and Fs were both digested with BglI (NEB) and BsaI (NEB). The Rs4231 S gene was digested with BsmBI. The Rs7327 S gene was digested with BsaI. The other fragments and bacterial artificial chromosome (BAC) were prepared as described previously. Then the two prepared spike DNA fragments were separately inserted into BAC with Es, Fs and other fragments. The correct infectious BAC clones were screened. The chimeric viruses were rescued as described previously [23]."
^^^^ This is exactly "genetic manipulation of SARS-related bat coronaviruses". — Preceding unsigned comment added by 193.165.236.120 ( talk • contribs)
This is the
talk page for discussing improvements to the
COVID-19 lab leak theory article. This is not a forum for general discussion of the article's subject. |
Article policies
|
Find medical sources: Source guidelines · PubMed · Cochrane · DOAJ · Gale · OpenMD · ScienceDirect · Springer · Trip · Wiley · TWL |
Archives: Index, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34Auto-archiving period: 30 days |
The contentious topics procedure applies to this page. This page is related to COVID-19, broadly construed, which is a contentious topic. Please consult the procedures and edit carefully. |
There have been attempts to
recruit editors of specific viewpoints to this article, in a manner that does not comply with Wikipedia's policies. Editors are encouraged to use neutral mechanisms for requesting outside input (e.g. a "
request for comment", a
third opinion or other noticeboard post, or neutral criteria: "pinging all editors who have edited this page in the last 48 hours"). If someone has asked you to provide your opinion here, examine the arguments, not the editors who have made them. Reminder: disputes are resolved by consensus, not by majority vote. |
This page is not a forum for general discussion about COVID-19 lab leak theory. Any such comments may be removed or refactored. Please limit discussion to improvement of this article. You may wish to ask factual questions about COVID-19 lab leak theory at the Reference desk. |
This article is rated C-class on Wikipedia's
content assessment scale. It is of interest to multiple WikiProjects. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
This article has previously been nominated to be moved.
Discussions:
|
This article was nominated for deletion on July 18, 2021. The result of the discussion was keep. |
This article has been
mentioned by multiple media organizations:
|
Daily pageviews of this article
A graph should have been displayed here but
graphs are temporarily disabled. Until they are enabled again, visit the interactive graph at
pageviews.wmcloud.org |
based in part on Shi [Zhengli]'s emailed answers." ( RfC, December 2021)
This section is pinned and will not be automatically archived. |
List of good sources with good coverage to help expand. Not necessarily for inclusion but just for consideration. Preferably not articles that just discuss a single quote/press conference. The long-style reporting would be even better. Feel free to edit directly to add to the list. ProcrastinatingReader ( talk) 17:39, 18 July 2021 (UTC)
Last updated by Julian Brown ( talk) 23:43, 12 November 2023 (UTC)
] · |
---|
For the relevant sourcing guideline, see WP:SCHOLARSHIP. For a database curated by the NCBI, see LitCoVID |
|
] · |
---|
For the relevant sourcing guideline, see WP:RSOPINION. |
|
] · |
---|
Keep in mind, these are primary sources and thus should be used with caution! |
|
References
Now that the main phase of the pandemic is in the rear view window, it's clear to me that the lab-leak advocacy group DRASTIC doesn't pass WP:SUSTAINED, and could adequately be covered in a few sentences in this article. Most of the sources in that article don't even mention DRASIC, but merely debunk claims made by its members. Hemiauchenia ( talk) 02:32, 16 March 2024 (UTC)
I think it would be relevant to add the submission date for Project DEFUSE which is 3/27/18 [6]? 牢记使命 ( talk) 14:54, 2 April 2024 (UTC)
The timing of DEFUSE so close to the pandemic is obviously suspicious← that smells ripely fringey. What's the source for that? How can a abortive proposal affect a pandemic? Bon courage ( talk) 16:50, 5 April 2024 (UTC)
The current version of article contains a phrase: "There is no evidence that any genetic manipulation or reverse genetics (a technique required to make chimeric viruses) of SARS-related bat coronaviruses was ever carried out at the WIV."
It is FALSE. There is (at least one) publicly available paper which proves the contrary.
https://doi.org/10.1371/journal.ppat.1006698 is an article from 2017 with (among others) authored by Daszak and Zheng-Li Shi (the head of the WIV). "Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus".
It contains this passage:
"Construction of recombinant viruses
Recombinant viruses with the S gene of the novel bat SARSr-CoVs and the backbone of the infectious clone of SARSr-CoV WIV1 were constructed using the reverse genetic system described previously [23] (S9 Fig). The fragments E and F were re-amplified with primer pairs (FE, 5’-AGGGCCCACCTGGCACTGGTAAGAGTCATTTTGC-3’, R-EsBsaI, 5’-ACTGGTCTCTTCGTTTAGTTATTAACTAAAATATCACTAGACACC-3’) and (F-FsBsaI, 5’-TGAGGTCTCCGAACTTATGGATTTGTTTATGAG-3’, RF, 5’-AGGTAGGCCTCTAGGGCAGCTAAC-3’), respectively. The products were named as fragment Es and Fs, which leave the spike gene coding region as an independent fragment. BsaI sites (5’-GGTCTCN|NNNN-3’) were introduced into the 3’ terminal of the Es fragment and the 5’ terminal of the Fs fragment, respectively. The spike sequence of Rs4231 was amplified with the primer pair (F-Rs4231-BsmBI, 5’-AGTCGTCTCAACGAACATGTTTATTTTCTTATTCTTTCTCACTCTCAC-3’ and R-Rs4231-BsmBI, 5’-TCACGTCTCAGTTCGTTTATGTGTAATGTAATTTGACACCCTTG-3’). The S gene sequence of Rs7327 was amplified with primer pair (F-Rs7327-BsaI, 5’-AGTGGTCTCAACGAACATGAAATTGTTAGTTTTAGTTTTTGCTAC-3’ and R-Rs7327-BsaI, 5’- TCAGGTCTCAGTTCGTTTATGTGTAATGTAATTTAACACCCTTG-3’). The fragment Es and Fs were both digested with BglI (NEB) and BsaI (NEB). The Rs4231 S gene was digested with BsmBI. The Rs7327 S gene was digested with BsaI. The other fragments and bacterial artificial chromosome (BAC) were prepared as described previously. Then the two prepared spike DNA fragments were separately inserted into BAC with Es, Fs and other fragments. The correct infectious BAC clones were screened. The chimeric viruses were rescued as described previously [23]."
^^^^ This is exactly "genetic manipulation of SARS-related bat coronaviruses". — Preceding unsigned comment added by 193.165.236.120 ( talk • contribs)