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This article was the subject of a Wiki Education Foundation-supported course assignment, between 22 August 2019 and 4 December 2019. Further details are available
on the course page. Student editor(s):
Avultaggio. Peer reviewers:
Kasarlo.
Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT ( talk) 07:39, 17 January 2022 (UTC)
The lead section follows style guidelines though I would strengthen it more by also including piwi-interacting RNA in the lead paragraph. Cited sources accurately represent the contents mentioned in the article and the writing is clear without excessive jargon and without ambiguity. Each section matches well with the section heading and the content is well-structured and written from a neutral point of view.
I would suggest amending the heading "Applications" to "Therapeutic Applications." Also, the author(s) may wish to add short hairpin RNA (shRNA) to the article. Pfeffer et al. found that shRNA is a valuable tool in genetics for technological and therapeutic applications in RNA silencing and you may find their work relevant to your article. Pfeffer et al. looked at the use of synthesized shRNA that are processed to siRNA to stably inhibit gene expression.
Works Cited:
Pfeffer et al. (2005). RNA silencing. B.I.F. Futura. 20. Retrieved on 2 May 2013 from < http://www.rockefeller.edu/labheads/tuschl/Pfeffer,BImeeting05.pdf>. Bmesido1 ( talk) 02:23, 3 May 2013 (UTC)
Hi, guys. It really looks like you've put a great deal of effort into your article. Congratulations! I have a few observations and a few suggestions for improvement to list; however, the article is looking very good. I want to begin by saying that, overall, the text is well-written and flows well. I like that you've incorporated at least one image into your article. There may be some places in the article for you to add additional images if something seems appropriate and will add meaningful support to the text. Just be careful to maintain an encyclopedic tone throughout the article. There are a couple of instances in the article that the phrase "at least" is used in a manner that is not necessary. A non-scientist would have a difficult time getting through this article. It makes perfect sense to us because we hear and see these terms every day. There may be some opportunities within the article to make things a little simpler without feeling like you're "dumbing it down" too much. Under the piRNA section, you briefly mention rasiRNAs at the end, and then don't explain what they are. That just leaves the reader hanging. There is a LOT of overlap between this article and the RNA Interference article. I realize that you don't want to be redundant and repeat what the other article already states, but the section on biological functions is almost completely referring the reader to another article. Brainstorm and see if you can come up with some novel content in this section. Also, there are some sections near the end of the article, such as the research section that have no content. As for the subheading for open ended questions, you may want to consider changing that to Ongoing Research or something similar since it closely follows the section addressing recent and current research. I hope this is helpful. Great job so far! Awotawa1 ( talk) 02:39, 3 May 2013 (UTC)
Martin, I am finalizing my new entries now. Also, I want to create a diagram and will do so today. My thought is that the best place to post it will most likely be in the "Mechanism" section. I will try to diagram something that complements the portions you have drafted and otherwise will incorporate some explanatory content. Ppelletier ( talk) 14:42, 26 April 2013 (UTC)
By reading a little more of the article "Genetics of RNA Silencing", I believe that the terms Post-Transcriptional Gene Silencing and cosupression were just used first, before the use of the word RNAi (check iRNA in 3rd paragraph of Background). There really isn't any difference in the mechanisms, so I won't add anything to that part. I'll add a link to the RNAi sub-article about the more-in-depth RNAi mechanism description. MartinLubell ( talk) 02:59, 26 April 2013 (UTC)
Groups of similar citations. (I'll take care of this once we are done.)
MartinLubell ( talk) 16:12, 25 April 2013 (UTC)
Hello Pauline, It's probably obvious, but when you search for "RNA Silencing" with Google, our article shows up first. That's pretty cool! I think we should make sure that there exists the link to the RNAi article right away, which is considered one of the best Wikipedia articles. (I checked, we do have the link, but maybe we can bring the 2nd paragraph as the 2nd sentence of the 1st paragraph?) MartinLubell ( talk) 16:00, 25 April 2013 (UTC)
The article has a lot of good information and the amount of sources are great. The material reads smoothly and is easy to comprehend. The only thing that I suggest that the group may want to reorganize/change is the part of the article were there is an overview, introduction, and summary. A different title for these section may help the awkwardness (for lack of a better word). Maybe merge the "Overview" and "Introduction" and title it "Background". This would give the different types of RNAi's could have a section title. I also have to agree with Lauren on the "Biological Functions" section, more research should be done on this section or maybe try to incorporate it into another section within the article. Tbrownc1 ( talk) 03:29, 12 April 2013 (UTC)
MartinLubell ( talk) 15:37, 25 April 2013 (UTC)
So I think overall this page is good. There is substantial information on the topic, but you don't lose focus. My only real issue is with the "summary" section. I think it needs a different name. Actually, if you just get rid of summary, that paragraph can stay under the introduction heading. Also, the first two paragraphs need to either be removed or incorporated into the rest of the page. They do not flow with the rest of the page and it seems disjointed. Finally, maybe expand a little more in the Biological Functions section; it seems light on information. Laurenwking ( talk) 01:24, 12 April 2013 (UTC)
Hello Pauline, in my sandbox, I wrote a rough draft of the mechanism of RNA Silencing, yeah, pretty bad, but anyway, I got something down, and I'll be continuing tomorrow with the biological functions. Once you've put up your stuff to the article (I'm assuming, or just in our group page?), I'll add my stuff. I don't want to overwrite your stuff or vice-versa : ) Good night, MartinLubell ( talk) 04:58, 5 April 2013 (UTC)
Hello Pauline, I added Prof. Ogg's quote 2nd paragraph. Feel free to move it around or integrate it in your overview. I like it at the beginning because it was something that I had a hard time understanding. "RNA silencing is a broader category than RNAi, and RNAi falls into the category of RNA silencing. However, having this distinction does not necessarily mean that everyone considers silencing and interference to be two separate entities. In much of the literature you will hear both terms used interchangeably." MartinLubell ( talk) 19:32, 5 April 2013 (UTC)
The following editors are currently dedicating time to improve this article.
Ppelletier ( talk) 17:26, 5 April 2013 (UTC)
In general, a unique issue with this topic is its breadth and potential overlap with iRNA, which encompasses miRNA and siRNA. Each of these topics have a respective entry on Wikipedia. "RNA silencing" is construed here as broader, although it would be helpful to have feedback on this construction from the community. The Overview section is intended to address RNA silencing and how it has evolved over the years, whether there are any other types of RNA silencing in addition to miRNA and siRNA, and beginning to craft some distinctions to the extent this is helpful in understanding RNA silencing as a broad topic covering a variety of mechanistically related pathways for controlling gene expression involving small RNA. Ppelletier ( talk) 17:26, 5 April 2013 (UTC)
(Copied list from Gene silencing article)
Our proposed outline is as follows, again feedback is requested from the community:
Ppelletier ( talk) 17:26, 5 April 2013 (UTC) Updates to outline. Ppelletier ( talk) 03:09, 26 April 2013 (UTC)
There are several more terms related to specific topics of gene silencing:
Transcriptional Gene Silencing:
Post-transcriptional Gene Silencing:
Meiotic gene silencing:
Cellular components of gene silencing:
Ribozymes, siRNA and antisense technologies such as Morpholino or PNA oligos are experimental approaches used to post-transcriptionally silence targeted genes of interest. — Preceding unsigned comment added by MartinLubell ( talk • contribs) 05:21, 14 March 2013 (UTC)
It might be confusing in the long run, but the latest subjects are at the top. MartinLubell ( talk) 03:26, 3 May 2013 (UTC)
These are two different things, with two separate articles, so I'm not sure why the lead sentence of the article implies that they are the same thing. Pariah24 ( talk) 18:12, 14 December 2018 (UTC)
Indeed, it is confusing. As an RNAi researcher, I used them interchangeably and didn't know the distinction. The RNA interference article also doesn't link to this page, which doesn't help. It might help to put in a "Terminology" subheader under "Background" Strongyloides ( talk) 21:32, 20 September 2021 (UTC)
![]() | This article is rated C-class on Wikipedia's
content assessment scale. It is of interest to the following WikiProjects: | |||||||||||||
|
![]() | This article links to one or more target anchors that no longer exist.
Please help fix the broken anchors. You can remove this template after fixing the problems. |
Reporting errors |
This article was the subject of a Wiki Education Foundation-supported course assignment, between 22 August 2019 and 4 December 2019. Further details are available
on the course page. Student editor(s):
Avultaggio. Peer reviewers:
Kasarlo.
Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT ( talk) 07:39, 17 January 2022 (UTC)
The lead section follows style guidelines though I would strengthen it more by also including piwi-interacting RNA in the lead paragraph. Cited sources accurately represent the contents mentioned in the article and the writing is clear without excessive jargon and without ambiguity. Each section matches well with the section heading and the content is well-structured and written from a neutral point of view.
I would suggest amending the heading "Applications" to "Therapeutic Applications." Also, the author(s) may wish to add short hairpin RNA (shRNA) to the article. Pfeffer et al. found that shRNA is a valuable tool in genetics for technological and therapeutic applications in RNA silencing and you may find their work relevant to your article. Pfeffer et al. looked at the use of synthesized shRNA that are processed to siRNA to stably inhibit gene expression.
Works Cited:
Pfeffer et al. (2005). RNA silencing. B.I.F. Futura. 20. Retrieved on 2 May 2013 from < http://www.rockefeller.edu/labheads/tuschl/Pfeffer,BImeeting05.pdf>. Bmesido1 ( talk) 02:23, 3 May 2013 (UTC)
Hi, guys. It really looks like you've put a great deal of effort into your article. Congratulations! I have a few observations and a few suggestions for improvement to list; however, the article is looking very good. I want to begin by saying that, overall, the text is well-written and flows well. I like that you've incorporated at least one image into your article. There may be some places in the article for you to add additional images if something seems appropriate and will add meaningful support to the text. Just be careful to maintain an encyclopedic tone throughout the article. There are a couple of instances in the article that the phrase "at least" is used in a manner that is not necessary. A non-scientist would have a difficult time getting through this article. It makes perfect sense to us because we hear and see these terms every day. There may be some opportunities within the article to make things a little simpler without feeling like you're "dumbing it down" too much. Under the piRNA section, you briefly mention rasiRNAs at the end, and then don't explain what they are. That just leaves the reader hanging. There is a LOT of overlap between this article and the RNA Interference article. I realize that you don't want to be redundant and repeat what the other article already states, but the section on biological functions is almost completely referring the reader to another article. Brainstorm and see if you can come up with some novel content in this section. Also, there are some sections near the end of the article, such as the research section that have no content. As for the subheading for open ended questions, you may want to consider changing that to Ongoing Research or something similar since it closely follows the section addressing recent and current research. I hope this is helpful. Great job so far! Awotawa1 ( talk) 02:39, 3 May 2013 (UTC)
Martin, I am finalizing my new entries now. Also, I want to create a diagram and will do so today. My thought is that the best place to post it will most likely be in the "Mechanism" section. I will try to diagram something that complements the portions you have drafted and otherwise will incorporate some explanatory content. Ppelletier ( talk) 14:42, 26 April 2013 (UTC)
By reading a little more of the article "Genetics of RNA Silencing", I believe that the terms Post-Transcriptional Gene Silencing and cosupression were just used first, before the use of the word RNAi (check iRNA in 3rd paragraph of Background). There really isn't any difference in the mechanisms, so I won't add anything to that part. I'll add a link to the RNAi sub-article about the more-in-depth RNAi mechanism description. MartinLubell ( talk) 02:59, 26 April 2013 (UTC)
Groups of similar citations. (I'll take care of this once we are done.)
MartinLubell ( talk) 16:12, 25 April 2013 (UTC)
Hello Pauline, It's probably obvious, but when you search for "RNA Silencing" with Google, our article shows up first. That's pretty cool! I think we should make sure that there exists the link to the RNAi article right away, which is considered one of the best Wikipedia articles. (I checked, we do have the link, but maybe we can bring the 2nd paragraph as the 2nd sentence of the 1st paragraph?) MartinLubell ( talk) 16:00, 25 April 2013 (UTC)
The article has a lot of good information and the amount of sources are great. The material reads smoothly and is easy to comprehend. The only thing that I suggest that the group may want to reorganize/change is the part of the article were there is an overview, introduction, and summary. A different title for these section may help the awkwardness (for lack of a better word). Maybe merge the "Overview" and "Introduction" and title it "Background". This would give the different types of RNAi's could have a section title. I also have to agree with Lauren on the "Biological Functions" section, more research should be done on this section or maybe try to incorporate it into another section within the article. Tbrownc1 ( talk) 03:29, 12 April 2013 (UTC)
MartinLubell ( talk) 15:37, 25 April 2013 (UTC)
So I think overall this page is good. There is substantial information on the topic, but you don't lose focus. My only real issue is with the "summary" section. I think it needs a different name. Actually, if you just get rid of summary, that paragraph can stay under the introduction heading. Also, the first two paragraphs need to either be removed or incorporated into the rest of the page. They do not flow with the rest of the page and it seems disjointed. Finally, maybe expand a little more in the Biological Functions section; it seems light on information. Laurenwking ( talk) 01:24, 12 April 2013 (UTC)
Hello Pauline, in my sandbox, I wrote a rough draft of the mechanism of RNA Silencing, yeah, pretty bad, but anyway, I got something down, and I'll be continuing tomorrow with the biological functions. Once you've put up your stuff to the article (I'm assuming, or just in our group page?), I'll add my stuff. I don't want to overwrite your stuff or vice-versa : ) Good night, MartinLubell ( talk) 04:58, 5 April 2013 (UTC)
Hello Pauline, I added Prof. Ogg's quote 2nd paragraph. Feel free to move it around or integrate it in your overview. I like it at the beginning because it was something that I had a hard time understanding. "RNA silencing is a broader category than RNAi, and RNAi falls into the category of RNA silencing. However, having this distinction does not necessarily mean that everyone considers silencing and interference to be two separate entities. In much of the literature you will hear both terms used interchangeably." MartinLubell ( talk) 19:32, 5 April 2013 (UTC)
The following editors are currently dedicating time to improve this article.
Ppelletier ( talk) 17:26, 5 April 2013 (UTC)
In general, a unique issue with this topic is its breadth and potential overlap with iRNA, which encompasses miRNA and siRNA. Each of these topics have a respective entry on Wikipedia. "RNA silencing" is construed here as broader, although it would be helpful to have feedback on this construction from the community. The Overview section is intended to address RNA silencing and how it has evolved over the years, whether there are any other types of RNA silencing in addition to miRNA and siRNA, and beginning to craft some distinctions to the extent this is helpful in understanding RNA silencing as a broad topic covering a variety of mechanistically related pathways for controlling gene expression involving small RNA. Ppelletier ( talk) 17:26, 5 April 2013 (UTC)
(Copied list from Gene silencing article)
Our proposed outline is as follows, again feedback is requested from the community:
Ppelletier ( talk) 17:26, 5 April 2013 (UTC) Updates to outline. Ppelletier ( talk) 03:09, 26 April 2013 (UTC)
There are several more terms related to specific topics of gene silencing:
Transcriptional Gene Silencing:
Post-transcriptional Gene Silencing:
Meiotic gene silencing:
Cellular components of gene silencing:
Ribozymes, siRNA and antisense technologies such as Morpholino or PNA oligos are experimental approaches used to post-transcriptionally silence targeted genes of interest. — Preceding unsigned comment added by MartinLubell ( talk • contribs) 05:21, 14 March 2013 (UTC)
It might be confusing in the long run, but the latest subjects are at the top. MartinLubell ( talk) 03:26, 3 May 2013 (UTC)
These are two different things, with two separate articles, so I'm not sure why the lead sentence of the article implies that they are the same thing. Pariah24 ( talk) 18:12, 14 December 2018 (UTC)
Indeed, it is confusing. As an RNAi researcher, I used them interchangeably and didn't know the distinction. The RNA interference article also doesn't link to this page, which doesn't help. It might help to put in a "Terminology" subheader under "Background" Strongyloides ( talk) 21:32, 20 September 2021 (UTC)