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Names | |
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IUPAC name
(E)-2,6-dimethyl-4-(2-(2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-9-yl)vinyl)pyrylium
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Other names | |
Identifiers | |
3D model (
JSmol)
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ChemSpider | |
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Properties | |
C21H24BF4NO | |
Molar mass | 393.23 g·mol−1 |
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
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Pyrylium-1 (Py-1) is a fluorogenic salt of a pyrylium derivative and tetrafluoroborate. It is an amine-labeling dye that is not fluorescent itself, but reacts with primary amines to form fluorescent products. [2] It is within the "chameleon labels" class, [1] so named due to their clear color-changing properties upon conjugation. Py-1 was first reported in 2004. [3] It has been used for the detection of amines and peptides, largely in CE-SDS, where it is recognized to reach a high sensitivity via laser-induced fluorescence. [2] Once bound to protein the excitation wavelength is 503 nm (green) and the emission wavelength is 603 nm (orange). [2] Similar to FQ, these fluorescence wavelengths makes Py-1 suitable for excitation with a 488 nm argon-ion laser.
Post conjugation, the Py-1 adduct (an addition of C21H21N) adds about 287.1674 Da to the target molecule.
![]() | |
Names | |
---|---|
IUPAC name
(E)-2,6-dimethyl-4-(2-(2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-9-yl)vinyl)pyrylium
| |
Other names | |
Identifiers | |
3D model (
JSmol)
|
|
ChemSpider | |
| |
| |
Properties | |
C21H24BF4NO | |
Molar mass | 393.23 g·mol−1 |
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
|
Pyrylium-1 (Py-1) is a fluorogenic salt of a pyrylium derivative and tetrafluoroborate. It is an amine-labeling dye that is not fluorescent itself, but reacts with primary amines to form fluorescent products. [2] It is within the "chameleon labels" class, [1] so named due to their clear color-changing properties upon conjugation. Py-1 was first reported in 2004. [3] It has been used for the detection of amines and peptides, largely in CE-SDS, where it is recognized to reach a high sensitivity via laser-induced fluorescence. [2] Once bound to protein the excitation wavelength is 503 nm (green) and the emission wavelength is 603 nm (orange). [2] Similar to FQ, these fluorescence wavelengths makes Py-1 suitable for excitation with a 488 nm argon-ion laser.
Post conjugation, the Py-1 adduct (an addition of C21H21N) adds about 287.1674 Da to the target molecule.