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Names | |
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IUPAC name
4'-phenylspiro[2-benzofuran-3,2'-furan]-1,3'-dione
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Other names
Fluram
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Identifiers | |
3D model (
JSmol)
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|
ChemSpider | |
ECHA InfoCard | 100.048.904 |
MeSH | D005450 |
PubChem
CID
|
|
UNII | |
CompTox Dashboard (
EPA)
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|
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Properties | |
C17H10O4 | |
Molar mass | 278.26 g/mol |
Melting point | 153 to 157 °C (307 to 315 °F; 426 to 430 K) |
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
|
Fluorescamine is a spiro compound that is not fluorescent itself, but reacts with primary amines to form highly fluorescent products, i.e. it is fluorogenic. It hence has been used as a reagent for the detection of amines and peptides. [2] 1-100 μg of protein and down to 10 pg of protein can be detected. [3] [4] Once bound to protein the excitation wavelength is 381 nm (near ultraviolet) and the emission wavelength is 470 nm (blue). [5] This method is found to suffer from high blanks resulting from a high rate of hydrolysis due to requiring a large excess concentration. [6] Alternative methods are based on ortho-phthalaldehyde (OPA), Ellman's reagent (DTNB), or epicocconone.
![]() | |
Names | |
---|---|
IUPAC name
4'-phenylspiro[2-benzofuran-3,2'-furan]-1,3'-dione
| |
Other names
Fluram
| |
Identifiers | |
3D model (
JSmol)
|
|
ChemSpider | |
ECHA InfoCard | 100.048.904 |
MeSH | D005450 |
PubChem
CID
|
|
UNII | |
CompTox Dashboard (
EPA)
|
|
| |
| |
Properties | |
C17H10O4 | |
Molar mass | 278.26 g/mol |
Melting point | 153 to 157 °C (307 to 315 °F; 426 to 430 K) |
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
|
Fluorescamine is a spiro compound that is not fluorescent itself, but reacts with primary amines to form highly fluorescent products, i.e. it is fluorogenic. It hence has been used as a reagent for the detection of amines and peptides. [2] 1-100 μg of protein and down to 10 pg of protein can be detected. [3] [4] Once bound to protein the excitation wavelength is 381 nm (near ultraviolet) and the emission wavelength is 470 nm (blue). [5] This method is found to suffer from high blanks resulting from a high rate of hydrolysis due to requiring a large excess concentration. [6] Alternative methods are based on ortho-phthalaldehyde (OPA), Ellman's reagent (DTNB), or epicocconone.