PDIA3, protein disulfide isomerase family A, member 3, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HEL-S-269, HEL-S-93n, HsT17083, P58, PI-PLC, protein disulfide isomerase family A member 3
Protein disulfide-isomerase A3 (PDIA3), also known as glucose-regulated protein, 58-kD (GRP58), is an
isomeraseenzyme encoded by the autosomal gene PDIA3 in humans.[5][6][7][8] This protein
localizes to the
endoplasmic reticulum (ER) and interacts with
lectinchaperonescalreticulin and
calnexin (CNX) to modulate folding of newly synthesized glycoproteins. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.[9]
Structure
The PDIA3 protein consists of four thioredoxin-like domains: a, b, b′, and a′. The a and a′ domains have Cys-Gly-His-Cys
active site motifs (C57-G58-H59-C60 and C406-G407-H408-C409) and are catalytically active.[10][11] The bb′ domains contain a CNX binding site, which is composed of positively charged, highly conserved residues (K214, K274, and R282) that interact with the negatively charged residues of the CNX P domain. The b′ domain comprises the majority of the binding site, but the β4-β5 loop of the b domain provides additional contact (K214) to strengthen the interaction.[11] A transient
disulfide bond forms between the
N-terminal cysteine in the catalytic motif and a substrate, but in a step called "escape pathway", the bond is disrupted as the
C-terminal cysteine attacks the N-terminal cysteine to release the substrate.[10]
Function
The PDIA3 protein is a
thioloxidoreductase that has
protein disulfide isomerase activity.[8][10] PDIA3 is also part of the
major histocompatibility complex (MHC) class I
peptide loading complex, which is essential for formation of the final antigen conformation and export from the
endoplasmic reticulum to the cell surface.[10][12] This protein of the endoplasmic reticulum interacts with lectin chaperones such as calreticulin and CNX in order to modulate the folding of proteins that are newly synthesized. It is believed that PDIA3 plays a role in protein folding by promoting the formation of disulfide bonds, and that CNX facilitates the positioning substrates next to the catalytic cysteines.[9][10] This function allows it to serve as a redox sensor by activating
mTORC1, which then mediates mTOR complex assembly to adapt cells to oxidative damage. Thus, PDIA3 regulates
cell growth and
death according to oxygen concentrations, such as in the
hypoxic microenvironment of bones. Additionally, PDIA3 activates cell anchorage in bones by associating with
cell division and
cytoskeleton proteins, such as beta-
actin and
vimentin, to form a complex which controls
TUBB3 folding and proper attachment of the
microtubules to the
kinetochore. PDIA3 also plays a role in
cytokine-dependent
signal transduction, including
STAT3 signaling.[13]
PDIA3 may also participate in
Vitamin D (specifically,
calcitriol) signaling as a membrane-bound receptor.[14]
Clinical significance
It has been demonstrated that the downregulation of ERp57 expression is correlated with poor prognosis in early-stage cervical
cancer.[15] It has also been demonstrated that ERp57/PDIA3 binds specific DNA fragments in a melanoma cell line.[16] PDIA3 is also involved in bone
metastasis, which is the most common source of distant relapse in
breast cancer.[13] In addition to cancer, overexpression of PDIA3 is linked to
renal fibrosis, which is characterized by excess synthesis and secretion of
ECM leading to ER stress.[17]
Interactions
It has been demonstrated that PDIA3 interacts with:
^Hirano N, Shibasaki F, Sakai R, Tanaka T, Nishida J, Yazaki Y, Takenawa T, Hirai H (Nov 1995). "Molecular cloning of the human glucose-regulated protein ERp57/GRP58, a thiol-dependent reductase. Identification of its secretory form and inducible expression by the oncogenic transformation". European Journal of Biochemistry. 234 (1): 336–42.
doi:
10.1111/j.1432-1033.1995.336_c.x.
PMID8529662.
^Garbi N, Tanaka S, Momburg F, Hämmerling GJ (Jan 2006). "Impaired assembly of the major histocompatibility complex class I peptide-loading complex in mice deficient in the oxidoreductase ERp57". Nature Immunology. 7 (1): 93–102.
doi:
10.1038/ni1288.
PMID16311600.
S2CID5857455.
^Aureli C, Gaucci E, Arcangeli V, Grillo C, Eufemi M, Chichiarelli S (Jul 2013). "ERp57/PDIA3 binds specific DNA fragments in a melanoma cell line". Gene. 524 (2): 390–5.
doi:
10.1016/j.gene.2013.04.004.
hdl:
11573/516861.
PMID23587917.
PDIA3, protein disulfide isomerase family A, member 3, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HEL-S-269, HEL-S-93n, HsT17083, P58, PI-PLC, protein disulfide isomerase family A member 3
Protein disulfide-isomerase A3 (PDIA3), also known as glucose-regulated protein, 58-kD (GRP58), is an
isomeraseenzyme encoded by the autosomal gene PDIA3 in humans.[5][6][7][8] This protein
localizes to the
endoplasmic reticulum (ER) and interacts with
lectinchaperonescalreticulin and
calnexin (CNX) to modulate folding of newly synthesized glycoproteins. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.[9]
Structure
The PDIA3 protein consists of four thioredoxin-like domains: a, b, b′, and a′. The a and a′ domains have Cys-Gly-His-Cys
active site motifs (C57-G58-H59-C60 and C406-G407-H408-C409) and are catalytically active.[10][11] The bb′ domains contain a CNX binding site, which is composed of positively charged, highly conserved residues (K214, K274, and R282) that interact with the negatively charged residues of the CNX P domain. The b′ domain comprises the majority of the binding site, but the β4-β5 loop of the b domain provides additional contact (K214) to strengthen the interaction.[11] A transient
disulfide bond forms between the
N-terminal cysteine in the catalytic motif and a substrate, but in a step called "escape pathway", the bond is disrupted as the
C-terminal cysteine attacks the N-terminal cysteine to release the substrate.[10]
Function
The PDIA3 protein is a
thioloxidoreductase that has
protein disulfide isomerase activity.[8][10] PDIA3 is also part of the
major histocompatibility complex (MHC) class I
peptide loading complex, which is essential for formation of the final antigen conformation and export from the
endoplasmic reticulum to the cell surface.[10][12] This protein of the endoplasmic reticulum interacts with lectin chaperones such as calreticulin and CNX in order to modulate the folding of proteins that are newly synthesized. It is believed that PDIA3 plays a role in protein folding by promoting the formation of disulfide bonds, and that CNX facilitates the positioning substrates next to the catalytic cysteines.[9][10] This function allows it to serve as a redox sensor by activating
mTORC1, which then mediates mTOR complex assembly to adapt cells to oxidative damage. Thus, PDIA3 regulates
cell growth and
death according to oxygen concentrations, such as in the
hypoxic microenvironment of bones. Additionally, PDIA3 activates cell anchorage in bones by associating with
cell division and
cytoskeleton proteins, such as beta-
actin and
vimentin, to form a complex which controls
TUBB3 folding and proper attachment of the
microtubules to the
kinetochore. PDIA3 also plays a role in
cytokine-dependent
signal transduction, including
STAT3 signaling.[13]
PDIA3 may also participate in
Vitamin D (specifically,
calcitriol) signaling as a membrane-bound receptor.[14]
Clinical significance
It has been demonstrated that the downregulation of ERp57 expression is correlated with poor prognosis in early-stage cervical
cancer.[15] It has also been demonstrated that ERp57/PDIA3 binds specific DNA fragments in a melanoma cell line.[16] PDIA3 is also involved in bone
metastasis, which is the most common source of distant relapse in
breast cancer.[13] In addition to cancer, overexpression of PDIA3 is linked to
renal fibrosis, which is characterized by excess synthesis and secretion of
ECM leading to ER stress.[17]
Interactions
It has been demonstrated that PDIA3 interacts with:
^Hirano N, Shibasaki F, Sakai R, Tanaka T, Nishida J, Yazaki Y, Takenawa T, Hirai H (Nov 1995). "Molecular cloning of the human glucose-regulated protein ERp57/GRP58, a thiol-dependent reductase. Identification of its secretory form and inducible expression by the oncogenic transformation". European Journal of Biochemistry. 234 (1): 336–42.
doi:
10.1111/j.1432-1033.1995.336_c.x.
PMID8529662.
^Garbi N, Tanaka S, Momburg F, Hämmerling GJ (Jan 2006). "Impaired assembly of the major histocompatibility complex class I peptide-loading complex in mice deficient in the oxidoreductase ERp57". Nature Immunology. 7 (1): 93–102.
doi:
10.1038/ni1288.
PMID16311600.
S2CID5857455.
^Aureli C, Gaucci E, Arcangeli V, Grillo C, Eufemi M, Chichiarelli S (Jul 2013). "ERp57/PDIA3 binds specific DNA fragments in a melanoma cell line". Gene. 524 (2): 390–5.
doi:
10.1016/j.gene.2013.04.004.
hdl:
11573/516861.
PMID23587917.