This is a list of instruments used in microbiological sterilization and disinfection. [1] [2] [3]
Instrument | Uses |
---|---|
Instrument sterilizers | Used to sterilize instruments in absence of an autoclave |
Dressing drums | storage of gowns, cotton, linen, etc. |
The microscope | used for visualising minute structures including microbes |
Various stains | used to stain microscopic slides to get contrast |
Hot air oven | used in sterilizing instruments for various aseptic procedures, specially if that can not be autoclaved like powders |
Koch's or Arnold's steam sterilizer | used for steam sterilization |
A pressure cooker | used as a portable autoclave |
Biological and chemical indicators | Used to ascertain if a certain process has been completed, e.g. spores used in an autoclave are killed if autoclaving is properly done |
Filters: | |
• Candle filter: | used as household water filters and as filters for large particles in the laboratories |
•• Diatomaceous earth filters like the Berkefeld filter | -do- |
••Unglazed porcelain filters like the Chamberland filter | -do- |
• Disk filter or Seitz filter | previously used as bacteriological filters; presently obsolete |
• Sintered glass filter | used as a good particle filter in laboratories |
• Membrane filter and Syringe filter | used as primary bacterial/cell filters in procedures as toxin, immunoglobulin, etc. production, where the product gets denatured on heating |
• Air filter | like HEPA filter, used in various laboratories and clean rooms to produce lamellar air flow |
Radiation: | |
• Gamma ray source | used in sterilization of heat-labile products like plastic or rubber syringes, catheters and gloves |
• X-ray source | -do- |
• Infrared light source | -do- |
• Ultraviolet light source | -do- |
Inspissator | used to produce culture media for bacteriology that contain egg or serum, which coagulate on heating |
Tyndallizer | a process of sterilization from spore bearing bacteria; video link |
Water bath | to heat things uniformly from all sides at a set temperature up to the boiling point of water |
Needle Destroyer | Burns the needle electrically either cuts the syringe manually or burns it electrically |
Sharps container [4] | A imperforable container for sharp wastes like needles, blades, microscope slides, broken glass, etc. |
Cardboard biomedical waste containers | |
Reusable tubs [5] | |
Colour coded biomedical waste bags (India) [6] | - |
•Yellow plastic bags | for human anatomical, animal, microbiological and soiled waste |
•Red disinfected container or plastic bags | microbiological waste, solid waste(IV tubes, catheters, etc.) |
•Blue or White plastic bag or puncture proof containers | sharps, disposable tubing, etc. |
•Black Plastic bag or puncture proof containers | discarded medicines, incineration ash, chemical waste |
Disinfectants | for cleaning |
Incinerators | to burn biomedical wastes like removed body parts, blood, gauze, linen, etc. |
This is a list of instruments used in microbiological sterilization and disinfection. [1] [2] [3]
Instrument | Uses |
---|---|
Instrument sterilizers | Used to sterilize instruments in absence of an autoclave |
Dressing drums | storage of gowns, cotton, linen, etc. |
The microscope | used for visualising minute structures including microbes |
Various stains | used to stain microscopic slides to get contrast |
Hot air oven | used in sterilizing instruments for various aseptic procedures, specially if that can not be autoclaved like powders |
Koch's or Arnold's steam sterilizer | used for steam sterilization |
A pressure cooker | used as a portable autoclave |
Biological and chemical indicators | Used to ascertain if a certain process has been completed, e.g. spores used in an autoclave are killed if autoclaving is properly done |
Filters: | |
• Candle filter: | used as household water filters and as filters for large particles in the laboratories |
•• Diatomaceous earth filters like the Berkefeld filter | -do- |
••Unglazed porcelain filters like the Chamberland filter | -do- |
• Disk filter or Seitz filter | previously used as bacteriological filters; presently obsolete |
• Sintered glass filter | used as a good particle filter in laboratories |
• Membrane filter and Syringe filter | used as primary bacterial/cell filters in procedures as toxin, immunoglobulin, etc. production, where the product gets denatured on heating |
• Air filter | like HEPA filter, used in various laboratories and clean rooms to produce lamellar air flow |
Radiation: | |
• Gamma ray source | used in sterilization of heat-labile products like plastic or rubber syringes, catheters and gloves |
• X-ray source | -do- |
• Infrared light source | -do- |
• Ultraviolet light source | -do- |
Inspissator | used to produce culture media for bacteriology that contain egg or serum, which coagulate on heating |
Tyndallizer | a process of sterilization from spore bearing bacteria; video link |
Water bath | to heat things uniformly from all sides at a set temperature up to the boiling point of water |
Needle Destroyer | Burns the needle electrically either cuts the syringe manually or burns it electrically |
Sharps container [4] | A imperforable container for sharp wastes like needles, blades, microscope slides, broken glass, etc. |
Cardboard biomedical waste containers | |
Reusable tubs [5] | |
Colour coded biomedical waste bags (India) [6] | - |
•Yellow plastic bags | for human anatomical, animal, microbiological and soiled waste |
•Red disinfected container or plastic bags | microbiological waste, solid waste(IV tubes, catheters, etc.) |
•Blue or White plastic bag or puncture proof containers | sharps, disposable tubing, etc. |
•Black Plastic bag or puncture proof containers | discarded medicines, incineration ash, chemical waste |
Disinfectants | for cleaning |
Incinerators | to burn biomedical wastes like removed body parts, blood, gauze, linen, etc. |