| Chlorophyllide a reductase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.3.7.15 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Chlorophyllide a reductase ( EC 1.3.7.15), also known as COR, is an enzyme with systematic name bacteriochlorophyllide-a:ferredoxin 7,8-oxidoreductase. [1] [2] It catalyses the following chemical reaction
- chlorophyllide a + 2 reduced ferredoxin + ATP + H2O + 2 H+ 3-deacetyl 3-vinylbacteriochlorophyllide a + 2 oxidized ferredoxin + ADP + phosphate
- Structure of the substrate and product of the enzyme
-
Chlorophyllide a -
Bacteriochlorophyllide a (R=H). The product of COR retains the vinyl group of the substrate in place of the acetyl group shown
This reduction (with trans stereochemistry) of the pyrrole ring B, gives the characteristic 18-electron aromatic system that distinguishes bacteriochlorophylls from chlorophylls, which retain the chlorin system of Chlorophyllide a. This enzyme is present in purple bacteria such as Rhodobacter capsulatus and Rhodobacter sphaeroides, and Pseudomonadota. It is a component of the biosynthetic pathway to bacteriochlorophylls. [3] [4] [5]
See also
References
- ^ Harada, Jiro; Mizoguchi, Tadashi; Tsukatani, Yusuke; Yokono, Makio; Tanaka, Ayumi; Tamiaki, Hitoshi (2014). "Chlorophyllide a Oxidoreductase Works as One of the Divinyl Reductases Specifically Involved in Bacteriochlorophyll a Biosynthesis". Journal of Biological Chemistry. 289 (18): 12716–12726. doi: 10.1074/jbc.M113.546739. PMC 4007461. PMID 24637023.
- ^ Tsukatani, Yusuke; Harada, Jiro; Nomata, Jiro; Yamamoto, Haruki; Fujita, Yuichi; Mizoguchi, Tadashi; Tamiaki, Hitoshi (2015). "Rhodobacter sphaeroides mutants overexpressing chlorophyllide a oxidoreductase of Blastochloris viridis elucidate functions of enzymes in late bacteriochlorophyll biosynthetic pathways". Scientific Reports. 5: 9741. Bibcode: 2015NatSR...5E9741T. doi: 10.1038/srep09741. PMC 4432870. PMID 25978726.
- ^ R. Caspi (2015-12-08). "Pathway: bacteriochlorophyll a biosynthesis". MetaCyc Metabolic Pathway Database. Retrieved 2020-06-04.
- ^ Willows RD (June 2003). "Biosynthesis of chlorophylls from protoporphyrin IX". Natural Product Reports. 20 (3): 327–41. doi: 10.1039/B110549N. PMID 12828371.
- ^ Bollivar DW (November 2006). "Recent advances in chlorophyll biosynthesis". Photosynthesis Research. 90 (2): 173–94. doi: 10.1007/s11120-006-9076-6. PMID 17370354. S2CID 23808539.
| Chlorophyllide a reductase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.3.7.15 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Chlorophyllide a reductase ( EC 1.3.7.15), also known as COR, is an enzyme with systematic name bacteriochlorophyllide-a:ferredoxin 7,8-oxidoreductase. [1] [2] It catalyses the following chemical reaction
- chlorophyllide a + 2 reduced ferredoxin + ATP + H2O + 2 H+ 3-deacetyl 3-vinylbacteriochlorophyllide a + 2 oxidized ferredoxin + ADP + phosphate
- Structure of the substrate and product of the enzyme
-
Chlorophyllide a
-
Bacteriochlorophyllide a (R=H). The product of COR retains the vinyl group of the substrate in place of the acetyl group shown
This reduction (with trans stereochemistry) of the pyrrole ring B, gives the characteristic 18-electron aromatic system that distinguishes bacteriochlorophylls from chlorophylls, which retain the chlorin system of Chlorophyllide a. This enzyme is present in purple bacteria such as Rhodobacter capsulatus and Rhodobacter sphaeroides, and Pseudomonadota. It is a component of the biosynthetic pathway to bacteriochlorophylls. [3] [4] [5]
See also
References
- ^ Harada, Jiro; Mizoguchi, Tadashi; Tsukatani, Yusuke; Yokono, Makio; Tanaka, Ayumi; Tamiaki, Hitoshi (2014). "Chlorophyllide a Oxidoreductase Works as One of the Divinyl Reductases Specifically Involved in Bacteriochlorophyll a Biosynthesis". Journal of Biological Chemistry. 289 (18): 12716–12726. doi: 10.1074/jbc.M113.546739. PMC 4007461. PMID 24637023.
- ^ Tsukatani, Yusuke; Harada, Jiro; Nomata, Jiro; Yamamoto, Haruki; Fujita, Yuichi; Mizoguchi, Tadashi; Tamiaki, Hitoshi (2015). "Rhodobacter sphaeroides mutants overexpressing chlorophyllide a oxidoreductase of Blastochloris viridis elucidate functions of enzymes in late bacteriochlorophyll biosynthetic pathways". Scientific Reports. 5: 9741. Bibcode: 2015NatSR...5E9741T. doi: 10.1038/srep09741. PMC 4432870. PMID 25978726.
- ^ R. Caspi (2015-12-08). "Pathway: bacteriochlorophyll a biosynthesis". MetaCyc Metabolic Pathway Database. Retrieved 2020-06-04.
- ^ Willows RD (June 2003). "Biosynthesis of chlorophylls from protoporphyrin IX". Natural Product Reports. 20 (3): 327–41. doi: 10.1039/B110549N. PMID 12828371.
- ^ Bollivar DW (November 2006). "Recent advances in chlorophyll biosynthesis". Photosynthesis Research. 90 (2): 173–94. doi: 10.1007/s11120-006-9076-6. PMID 17370354. S2CID 23808539.