CDT1 (Chromatin licensing and DNA replication factor 1) is a
protein that in humans is encoded by the CDT1gene.[5][6][7][8] It is a
licensing factor that functions to limit DNA from replicating more than once per cell cycle.
Role in pre-replication complexes
The protein encoded by this gene is a key
licensing factor in the assembly of
pre-replication complexes (pre-RC), which occurs during the G1 phase of the cell cycle. In the assembly of pre-RCs, origin recognition complexes (ORC1-6) recognize and bind to DNA replication origins. CDT1, along with the protein
CDC6, are then recruited to the forming pre-RC, followed by minichromosome maintenance complexes (MCM2-7).[9]
The activity of CDT1 during the
cell cycle is tightly regulated during the S phase by the protein
geminin, which inhibits it, and by SCFSKP2, which ubiquinates the protein to tag it for proteasomal degradation.[10] This regulation is important in preventing relicensing, thus ensuring that DNA is only replicated once per cell cycle.
Orthologs
CDT1 belongs to a family of replication proteins conserved from yeast to humans. Examples of
orthologs in other species include:
S. pombe – CDT1 (CDC10-dependent transcript 1)[11]
DNA replication factor CDT1 has been shown to
interact with
SKP2.[14] Cdt1 is recruited by the
origin recognition complex in origin licensing. Null-mutations for CDT1 are lethal in yeast; the spores undergo mitosis without DNA replication. The overexpression of CDT1 causes rereplication in H. sapiens, which activates the
Chk1 pathway, preventing entry into mitosis.[15]
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^Rialland M, Sola F, Santocanale C (March 2002). "Essential role of human CDT1 in DNA replication and chromatin licensing". J Cell Sci. 115 (Pt 7): 1435–40.
doi:
10.1242/jcs.115.7.1435.
PMID11896191.
CDT1 (Chromatin licensing and DNA replication factor 1) is a
protein that in humans is encoded by the CDT1gene.[5][6][7][8] It is a
licensing factor that functions to limit DNA from replicating more than once per cell cycle.
Role in pre-replication complexes
The protein encoded by this gene is a key
licensing factor in the assembly of
pre-replication complexes (pre-RC), which occurs during the G1 phase of the cell cycle. In the assembly of pre-RCs, origin recognition complexes (ORC1-6) recognize and bind to DNA replication origins. CDT1, along with the protein
CDC6, are then recruited to the forming pre-RC, followed by minichromosome maintenance complexes (MCM2-7).[9]
The activity of CDT1 during the
cell cycle is tightly regulated during the S phase by the protein
geminin, which inhibits it, and by SCFSKP2, which ubiquinates the protein to tag it for proteasomal degradation.[10] This regulation is important in preventing relicensing, thus ensuring that DNA is only replicated once per cell cycle.
Orthologs
CDT1 belongs to a family of replication proteins conserved from yeast to humans. Examples of
orthologs in other species include:
S. pombe – CDT1 (CDC10-dependent transcript 1)[11]
DNA replication factor CDT1 has been shown to
interact with
SKP2.[14] Cdt1 is recruited by the
origin recognition complex in origin licensing. Null-mutations for CDT1 are lethal in yeast; the spores undergo mitosis without DNA replication. The overexpression of CDT1 causes rereplication in H. sapiens, which activates the
Chk1 pathway, preventing entry into mitosis.[15]
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^Rialland M, Sola F, Santocanale C (March 2002). "Essential role of human CDT1 in DNA replication and chromatin licensing". J Cell Sci. 115 (Pt 7): 1435–40.
doi:
10.1242/jcs.115.7.1435.
PMID11896191.