| 23S rRNA (uracil1939-C5)-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.190 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
23S rRNA (uracil1939-C5)-methyltransferase ( EC 2.1.1.190, RumA, RNA uridine methyltransferase A, YgcA) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (uracil1939-C5)-methyltransferase. [1] [2] [3] [4] [5] [6] This enzyme catalyses the following chemical reaction
- S-adenosyl-L-methionine + uracil1939 in 23S rRNA S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA
The enzyme specifically methylates uracil1939 at C5 in 23S rRNA.
References
- ^ Agarwalla S, Kealey JT, Santi DV, Stroud RM (March 2002). "Characterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli". The Journal of Biological Chemistry. 277 (11): 8835–40. doi: 10.1074/jbc.M111825200. PMID 11779873.
- ^ Lee TT, Agarwalla S, Stroud RM (March 2004). "Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase". Structure. 12 (3): 397–407. doi: 10.1016/j.str.2004.02.009. PMID 15016356.
- ^ Madsen CT, Mengel-Jørgensen J, Kirpekar F, Douthwaite S (August 2003). "Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry". Nucleic Acids Research. 31 (16): 4738–46. doi: 10.1093/nar/gkg657. PMC 169892. PMID 12907714.
- ^ Persaud C, Lu Y, Vila-Sanjurjo A, Campbell JL, Finley J, O'Connor M (February 2010). "Mutagenesis of the modified bases, m(5)U1939 and psi2504, in Escherichia coli 23S rRNA". Biochemical and Biophysical Research Communications. 392 (2): 223–7. doi: 10.1016/j.bbrc.2010.01.021. PMID 20067766.
- ^ Agarwalla S, Stroud RM, Gaffney BJ (August 2004). "Redox reactions of the iron-sulfur cluster in a ribosomal RNA methyltransferase, RumA: optical and EPR studies". The Journal of Biological Chemistry. 279 (33): 34123–9. doi: 10.1074/jbc.M405702200. PMC 1237038. PMID 15181002.
- ^ Lee TT, Agarwalla S, Stroud RM (March 2005). "A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function". Cell. 120 (5): 599–611. doi: 10.1016/j.cell.2004.12.037. PMID 15766524.
External links
- 23S+rRNA+(uracil1939-C5)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
| 23S rRNA (uracil1939-C5)-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.190 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
23S rRNA (uracil1939-C5)-methyltransferase ( EC 2.1.1.190, RumA, RNA uridine methyltransferase A, YgcA) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (uracil1939-C5)-methyltransferase. [1] [2] [3] [4] [5] [6] This enzyme catalyses the following chemical reaction
- S-adenosyl-L-methionine + uracil1939 in 23S rRNA S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA
The enzyme specifically methylates uracil1939 at C5 in 23S rRNA.
References
- ^ Agarwalla S, Kealey JT, Santi DV, Stroud RM (March 2002). "Characterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli". The Journal of Biological Chemistry. 277 (11): 8835–40. doi: 10.1074/jbc.M111825200. PMID 11779873.
- ^ Lee TT, Agarwalla S, Stroud RM (March 2004). "Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase". Structure. 12 (3): 397–407. doi: 10.1016/j.str.2004.02.009. PMID 15016356.
- ^ Madsen CT, Mengel-Jørgensen J, Kirpekar F, Douthwaite S (August 2003). "Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry". Nucleic Acids Research. 31 (16): 4738–46. doi: 10.1093/nar/gkg657. PMC 169892. PMID 12907714.
- ^ Persaud C, Lu Y, Vila-Sanjurjo A, Campbell JL, Finley J, O'Connor M (February 2010). "Mutagenesis of the modified bases, m(5)U1939 and psi2504, in Escherichia coli 23S rRNA". Biochemical and Biophysical Research Communications. 392 (2): 223–7. doi: 10.1016/j.bbrc.2010.01.021. PMID 20067766.
- ^ Agarwalla S, Stroud RM, Gaffney BJ (August 2004). "Redox reactions of the iron-sulfur cluster in a ribosomal RNA methyltransferase, RumA: optical and EPR studies". The Journal of Biological Chemistry. 279 (33): 34123–9. doi: 10.1074/jbc.M405702200. PMC 1237038. PMID 15181002.
- ^ Lee TT, Agarwalla S, Stroud RM (March 2005). "A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function". Cell. 120 (5): 599–611. doi: 10.1016/j.cell.2004.12.037. PMID 15766524.
External links
- 23S+rRNA+(uracil1939-C5)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)