From Wikipedia, the free encyclopedia
Class of enzymes
23S rRNA (adenine2503-C8)-methyltransferase (
EC
2.1.1.224, Cfr (gene)) is an
enzyme with
systematic name S-adenosyl-L-methionine:23S rRNA (adenine2503-C8)-methyltransferase.
[1]
[2]
[3]
[4]
[5]
[6] This enzyme
catalyses the following
chemical reaction
- 2
S-adenosyl-L-methionine +
adenine2503 in 23S rRNA
S-adenosyl-L-homocysteine + L-
methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
This enzyme is a member of the 'AdoMet radical' (radical SAM) family.
References
-
^ Giessing AM, Jensen SS, Rasmussen A, Hansen LH, Gondela A, Long K, Vester B, Kirpekar F (February 2009).
"Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria". RNA. 15 (2): 327–36.
doi:
10.1261/rna.1371409.
PMC
2648713.
PMID
19144912.
-
^ Kaminska KH, Purta E, Hansen LH, Bujnicki JM, Vester B, Long KS (March 2010).
"Insights into the structure, function and evolution of the radical-SAM 23S rRNA methyltransferase Cfr that confers antibiotic resistance in bacteria". Nucleic Acids Research. 38 (5): 1652–63.
doi:
10.1093/nar/gkp1142.
PMC
2836569.
PMID
20007606.
-
^ Yan F, LaMarre JM, Röhrich R, Wiesner J, Jomaa H, Mankin AS, Fujimori DG (March 2010).
"RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA". Journal of the American Chemical Society. 132 (11): 3953–64.
doi:
10.1021/ja910850y.
PMC
2859901.
PMID
20184321.
-
^ Yan F, Fujimori DG (March 2011).
"RNA methylation by radical SAM enzymes RlmN and Cfr proceeds via methylene transfer and hydride shift". Proceedings of the National Academy of Sciences of the United States of America. 108 (10): 3930–4.
Bibcode:
2011PNAS..108.3930Y.
doi:
10.1073/pnas.1017781108.
PMC
3054002.
PMID
21368151.
-
^ Grove TL, Benner JS, Radle MI, Ahlum JH, Landgraf BJ, Krebs C, Booker SJ (April 2011).
"A radically different mechanism for S-adenosylmethionine-dependent methyltransferases". Science. 332 (6029): 604–7.
Bibcode:
2011Sci...332..604G.
doi:
10.1126/science.1200877.
PMID
21415317.
S2CID
2214309.
-
^ Boal AK, Grove TL, McLaughlin MI, Yennawar NH, Booker SJ, Rosenzweig AC (May 2011).
"Structural basis for methyl transfer by a radical SAM enzyme". Science. 332 (6033): 1089–92.
Bibcode:
2011Sci...332.1089B.
doi:
10.1126/science.1205358.
PMC
3506250.
PMID
21527678.
External links
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