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According to this article on lentiviral packaging, safe lentiviral pseudoviruses can be produced by excluding gag/pol etc. from the viral genome proper and incorporating them instead with plasmids. These genes will not be packaged into the pseudoviruses because they lack "the HIV-1 elements that must be present in lentiviral vectors" which "include the RNA packaging signal (Ψ), the major splice donor, and the Rev-response element (RRE)." I get what the Ψ packaging signal is, as well as the RRE, but what is the major splice donor? I am surprised we do not have an article for it. Yanping Nora Soong ( talk) 09:13, 25 May 2020 (UTC)
Science desk | ||
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< May 24 | << Apr | May | Jun >> | May 26 > |
Welcome to the Wikipedia Science Reference Desk Archives |
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The page you are currently viewing is a transcluded archive page. While you can leave answers for any questions shown below, please ask new questions on one of the current reference desk pages. |
According to this article on lentiviral packaging, safe lentiviral pseudoviruses can be produced by excluding gag/pol etc. from the viral genome proper and incorporating them instead with plasmids. These genes will not be packaged into the pseudoviruses because they lack "the HIV-1 elements that must be present in lentiviral vectors" which "include the RNA packaging signal (Ψ), the major splice donor, and the Rev-response element (RRE)." I get what the Ψ packaging signal is, as well as the RRE, but what is the major splice donor? I am surprised we do not have an article for it. Yanping Nora Soong ( talk) 09:13, 25 May 2020 (UTC)