| Tournament details | |
|---|---|
| Country | 
England |
| Teams | 22 |
| Defending champions | Doncaster Rovers |
| Final positions | |
| Champions |
Chester City (1st title) |
| Runner-up | Kingstonian |
| Tournament statistics | |
| Matches played | 23 |
| Goals scored | 68 (2.96 per match) |
The 2000–01 Conference League Cup, known as the Nationwide Variety Club Trophy for sponsorship reasons, was a football tournament for clubs competing in that season's Football Conference. It was the 22nd edition of the tournament, and was thought to be the last after being axed, but it would briefly be reformed in 2004–05. It began withe the first round in September 2000, and concluded with the final on 7 May 2001.
Doncaster Rovers were the defending champions, but they were eliminated by Southport in the second round on 17 October 2000.
Calendar and prizes
| Round | Main date | New entries this round | Winners from previous round | Number of fixtures | Prize money |
| First Round | 18 September 2000 | 12 | none | 6 | N/A |
| Second Round | 9 October 2000 | 10 | 6 | 8 | N/A |
| Quarter Finals | 4 December 2000 | none | 8 | 4 | N/A |
| Semi-Finals | 2001 | none | 4 | 2 | N/A |
| Final | 7 May 2001 | none | 2 | 1 | N/A |
First Round
Northern Section
| 17 October 2000 | Northwich Victoria | 3–3 | Leigh RMI | Northwich |
| Report |
Ridings  33'Kielty 69', 86' |
Stadium:
Drill Field Attendance: 309 |
| 23 January 2001 Replay | Leigh RMI | 1–0 | Northwich Victoria | Leigh |
| Kielty | Report | Stadium:
Hilton Park Attendance: 161 |
| Tournament details | |
|---|---|
| Country |
England |
| Teams | 22 |
| Defending champions | Doncaster Rovers |
| Final positions | |
| Champions |
Chester City (1st title) |
| Runner-up | Kingstonian |
| Tournament statistics | |
| Matches played | 23 |
| Goals scored | 68 (2.96 per match) |
The 2000–01 Conference League Cup, known as the Nationwide Variety Club Trophy for sponsorship reasons, was a football tournament for clubs competing in that season's Football Conference. It was the 22nd edition of the tournament, and was thought to be the last after being axed, but it would briefly be reformed in 2004–05. It began withe the first round in September 2000, and concluded with the final on 7 May 2001.
Doncaster Rovers were the defending champions, but they were eliminated by Southport in the second round on 17 October 2000.
Calendar and prizes
| Round | Main date | New entries this round | Winners from previous round | Number of fixtures | Prize money |
| First Round | 18 September 2000 | 12 | none | 6 | N/A |
| Second Round | 9 October 2000 | 10 | 6 | 8 | N/A |
| Quarter Finals | 4 December 2000 | none | 8 | 4 | N/A |
| Semi-Finals | 2001 | none | 4 | 2 | N/A |
| Final | 7 May 2001 | none | 2 | 1 | N/A |
First Round
Northern Section
| 17 October 2000 | Northwich Victoria | 3–3 | Leigh RMI | Northwich |
| Report |
Ridings 33' Kielty 69', 86' |
Stadium:
Drill Field Attendance: 309 |
| 23 January 2001 Replay | Leigh RMI | 1–0 | Northwich Victoria | Leigh |
| Kielty | Report | Stadium:
Hilton Park Attendance: 161 |