| Nitrospira moscoviensis | |
|---|---|
| Scientific classification | |
| Kingdom: | |
| Phylum: | |
| Class: | |
| Order: | |
| Family: | |
| Genus: | |
| Species: | N. moscoviensis
|
| Binomial name | |
| Nitrospira moscoviensis Garrity et al. 2001
[1]
| |
Nitrospira moscoviensis was the second bacterium classified under the most diverse nitrite-oxidizing bacteria phylum, Nitrospirae.
[2]
[3] It is a
gram-negative, non-motile,
facultative lithoauthotropic bacterium that was discovered in
Moscow, Russia in 1995.
[2] The genus name,
Nitrospira, originates from the prefix “nitro” derived from nitrite, the microbe’s
electron donor and “spira” meaning coil or
spiral derived from the microbe’s shape.
[4] The species name, moscoviensis, is derived from Moscow, where the species was first discovered.
[4] N. moscoviensis could potentially be used in the production of
bio-degradable polymers.
[2]
In 1995, Silke Ehrich discovered Nitrospira moscoviensis in a sample taken from an eroded iron pipe. [2] The pipe was a part of a heating system in Moscow, Russia. [2] The rust was transferred to a culture where cells could be isolated. [2] For optimum growth, Ehrich and his team cultivated the cells on a mineral salt medium at a temperature of 39° C and at a pH of 7.6-8.0. [2]
Nitrospira moscoviensis is classified as being gram-negative, non-motile, and having a curved rod shape. [2] The curved rods are approximately 0.9-2.2 µm long x 0.2-0.4 µm wide. [2] N. moscoviensis can exist in both aquatic and terrestrial habitats and reproduces using binary fission. [2] Defining features of N. moscoviensis is the absence of intra- cytoplasmic membranes and carboxysomes possession of a flatulent periplasmic space. [5]
Nitrospira moscoviensis is a facultative lithoautotroph commonly referred to as a chemolithoautotroph. [2] In aerobic environments, N. moscoviensis obtains energy by oxidizing nitrite to nitrate. [5] Without the element molybdenum, the nitrite-oxidizing system will not function. [5] When N. moscoviensis is in nitrite free environments it can use aerobic hydrogen oxidation. [3] When N. moscoviensis reduces nitrite using hydrogen as an electron donor growth is blocked. [3] A key difference in N. moscoviensis’ nitrite-oxidizing system is location; unlike most nitrate oxidizing systems, it is not located in the cytoplasmic membrane. [5] Kirstein and Bock (1993) implied that the location of the nitrite-oxidizing system corresponds directly to N. moscoviensis having an enlarged periplasmic space. [6] By oxidizing nitrate outside of the cytoplasmic membrane, a permease nitrite system is not needed for the proton gradient. [5] The exocytoplasmic oxidation of nitrite also prevents build-up of toxic nitrite within the cytoplasm. [5] Another important metabolism ability for N. moscoviensis is its ability to cleave urea to ammonia and CO2. [3] The ability to use urea comes directly from the presence of urease encoding genes which is interesting because most nitrite-oxidizing bacteria are unable to use ammonia as an energy source. [3] Urease encoding genes function by catalyzing urea hydrolysis to form ammonia and carbamate. [3]
Nitrospira moscoviensis grows in temperatures from 33 to 40°C and pH 7.6-8.0 with an optimal nitrite concentration of 0.35 nM. [2] Nitrospira moscoviensis plays a key role in the two-step Nitrogen Cycle process. [3] The first step of Nitrification requires an ammonia-oxidizing bacterium (AOB) or ammonia-oxidizing archaeon (AOA) followed by a nitrite-oxidizing bacterium (NOB). [3] The unique capability of N. moscoviensis to cleave urea into ammonia and carbon dioxide allows for a symbiotic relationship with ammonia-oxidizing microorganisms (AOM) that lack this urease-production ability also know as negative AOM. [3] A correlation in environment preferences between Nitrospira species with nxrB gene encoding the β-subunit of nitro-oxidoreductase and AOM species with amoA gene further confirmed this relationship. [7] N. moscoviensis provides ammonia via hydrolysis of urea to these ammonia-oxidizing microorganisms which in turn produce nitrite, the primary energy source of N. moscoviensis. [3] The relationship between ureolytic nitrite-oxidizing bacteria and negative AOM is called reciprocal feeding. [3] Thus far, Nitrospira species have been recognized in natural environments as the primary vehicle for nitrite oxidation including soils, activated-sludge, ocean and fresh water, hot springs, and water treatment plants. [8]
Following its isolation, N. moscoviensis’s genome was sequenced by Dr. Ehrich et al. [2] Its 4.59 Mb genome has a GC content of 56.9+/-0.4 mol% with a predicted 4,863 coding sequences. [2] [3] N. moscoviensis's 16S rRNA gene sequences were found to be 88.9% similar to N. marina’s. [2] Despite its relatively low similarity to N. marina, N. moscoviensis was classified within the Nitrospirae phylum primarily due to shared morphological features including the presence of an enlarged periplasmic space. [2]
N. moscoviensis’s fully sequenced genome has provided useful phylogenetic insights beyond the scope of 16S rRNA sequence studies. [7] The discovery of the gene encoding the β-subunit of nitrite- oxidoreductase, nxrB, from N. moscoviensis as a functional genetic marker of Nitrospira, not only confirmed previous 16S rRNA phylogenetic classifications within the phylum, but revealed a new understanding of Nitrospira’s richness in terrestrial environments. [7] The phylum has expanded from two bacteria, N. marina and N. moscoviensis, to a 6-branched genera composed of a characteristically diverse group of nitrite-oxidizing bacteria with N. moscoviensis positioned in lineage II. [8]
The cytoplasm of Nitrospira moscoviensis contains polyhydroxybutyrate (PHB) granules. [2] PHB granules are polyhydroxyalkanoate (PHA) polymers. [9] PHB granules are produced by N. moscoviensis when the presence of nitrate is limited. [9] When nutrient limitations are no longer present, N. moscoviensis degrades PHB granules using enzymes, and recycling the degraded materials for functional use as a carbon source. [9] Synthetic polymers are used to make most plastics, synthetic polymers are non-biodegradable and contribute negatively to the environment. [9] Unlike synthetic polymers polyhydroxybutyrate is a biopolymer, meaning it can be bio-degraded. [9] PHB can be utilized for packaging, medical purposes like reconstructive surgery, and personal hygiene products. [9]
- ^ Garrity, George; Castenholz, Richard W.; Boone, David R., eds. (2001). Bergey's Manual of Systematic Bacteriology (2nd ed.). New York, NY: New York, NY. pp. 451–453. ISBN 978-0-387-21609-6.
- ^ a b c d e f g h i j k l m n o p q Ehrich, S; Behrens, D; Ludwig, W; Bock, E (1995). "A new obligately chemolithoautotrophic, nitrite-oxidizing bacterium, nitrospira moscoviensis sp. nov. and its phylogenetic relationship". Arch Microbiol. 164 (1): 16–23. doi: 10.1007/BF02568729.
- ^ a b c d e f g h i j k l Koch, H.; Luecker, S.; Albertsen, M.; Kitzinger, K.; Herbold, K.; Spieck, E.; Daims, H. (2015). "Expanded metabolic versatility of ubiquitous nitrite-oxidizing bacteria from the genus nitrospira". Proceedings of the National Academy of Sciences, USA. 112 (36).
- ^ a b Watson, S.W.; Bock, E.; Valois, F.W.; Waterbury, J.B.; Schlosser, U (1986). "Nitrospira marina gen. nov. sp. nov.: a chemolitho- trophic nitrite-oxidizing bacterium". Arch Microbiol. 144 (1): 1–7. doi: 10.1007/BF00454947.
- ^ a b c d e f Spieck, E.; Ehrich, S; Aamand, J; Bock, E. (1998). "Isolation and immunocytochemical location of the nitrite-oxidizing system in nitrospira moscoviensis". Arch Microbiol. 169 (3): 225–230. doi: 10.1007/s002030050565.
- ^ Kirstein, K; Bock, E (1993). "Close genetic relationship between Ni- trobacter hamburgensis nitrite oxidoreductase and Escherichia coli nitrate reductases". Arch Microbiol. 160 (6): 447–453. doi: 10.1007/BF00245305.
- ^ a b c Pester, Michael; Maixner, Frank; Berry, David; Rattei, Thomas; Koch, Hanna; Lücker, Sebastian; Nowka, Boris; Richter, Andreas; Spieck, Eva (2014-10-01). "NxrB encoding the beta subunit of nitrite oxidoreductase as functional and phylogenetic marker for nitrite-oxidizing Nitrospira". Environmental Microbiology. 16 (10): 3055–3071. doi: 10.1111/1462-2920.12300. ISSN 1462-2920.
- ^ a b Nowka, Boris; Off, Sandra; Daims, Holger; Spieck, Eva (2015-03-01). "Improved isolation strategies allowed the phenotypic differentiation of two Nitrospira strains from widespread phylogenetic lineages". FEMS Microbiology Ecology. 91 (3): fiu031. doi: 10.1093/femsec/fiu031. ISSN 1574-6941. PMID 25764560.
- ^ a b c d e f Ojumu, T.V.; Solomon, B.O (2004). "Production of Polyhydroxyalkanoates, a bacterial biodegradable polymer" (PDF). African Journal of Biotechnology. 3 (1): 18–24.
| Nitrospira moscoviensis | |
|---|---|
| Scientific classification | |
| Kingdom: | |
| Phylum: | |
| Class: | |
| Order: | |
| Family: | |
| Genus: | |
| Species: | N. moscoviensis
|
| Binomial name | |
| Nitrospira moscoviensis Garrity et al. 2001
[1]
| |
Nitrospira moscoviensis was the second bacterium classified under the most diverse nitrite-oxidizing bacteria phylum, Nitrospirae.
[2]
[3] It is a
gram-negative, non-motile,
facultative lithoauthotropic bacterium that was discovered in
Moscow, Russia in 1995.
[2] The genus name,
Nitrospira, originates from the prefix “nitro” derived from nitrite, the microbe’s
electron donor and “spira” meaning coil or
spiral derived from the microbe’s shape.
[4] The species name, moscoviensis, is derived from Moscow, where the species was first discovered.
[4] N. moscoviensis could potentially be used in the production of
bio-degradable polymers.
[2]
In 1995, Silke Ehrich discovered Nitrospira moscoviensis in a sample taken from an eroded iron pipe. [2] The pipe was a part of a heating system in Moscow, Russia. [2] The rust was transferred to a culture where cells could be isolated. [2] For optimum growth, Ehrich and his team cultivated the cells on a mineral salt medium at a temperature of 39° C and at a pH of 7.6-8.0. [2]
Nitrospira moscoviensis is classified as being gram-negative, non-motile, and having a curved rod shape. [2] The curved rods are approximately 0.9-2.2 µm long x 0.2-0.4 µm wide. [2] N. moscoviensis can exist in both aquatic and terrestrial habitats and reproduces using binary fission. [2] Defining features of N. moscoviensis is the absence of intra- cytoplasmic membranes and carboxysomes possession of a flatulent periplasmic space. [5]
Nitrospira moscoviensis is a facultative lithoautotroph commonly referred to as a chemolithoautotroph. [2] In aerobic environments, N. moscoviensis obtains energy by oxidizing nitrite to nitrate. [5] Without the element molybdenum, the nitrite-oxidizing system will not function. [5] When N. moscoviensis is in nitrite free environments it can use aerobic hydrogen oxidation. [3] When N. moscoviensis reduces nitrite using hydrogen as an electron donor growth is blocked. [3] A key difference in N. moscoviensis’ nitrite-oxidizing system is location; unlike most nitrate oxidizing systems, it is not located in the cytoplasmic membrane. [5] Kirstein and Bock (1993) implied that the location of the nitrite-oxidizing system corresponds directly to N. moscoviensis having an enlarged periplasmic space. [6] By oxidizing nitrate outside of the cytoplasmic membrane, a permease nitrite system is not needed for the proton gradient. [5] The exocytoplasmic oxidation of nitrite also prevents build-up of toxic nitrite within the cytoplasm. [5] Another important metabolism ability for N. moscoviensis is its ability to cleave urea to ammonia and CO2. [3] The ability to use urea comes directly from the presence of urease encoding genes which is interesting because most nitrite-oxidizing bacteria are unable to use ammonia as an energy source. [3] Urease encoding genes function by catalyzing urea hydrolysis to form ammonia and carbamate. [3]
Nitrospira moscoviensis grows in temperatures from 33 to 40°C and pH 7.6-8.0 with an optimal nitrite concentration of 0.35 nM. [2] Nitrospira moscoviensis plays a key role in the two-step Nitrogen Cycle process. [3] The first step of Nitrification requires an ammonia-oxidizing bacterium (AOB) or ammonia-oxidizing archaeon (AOA) followed by a nitrite-oxidizing bacterium (NOB). [3] The unique capability of N. moscoviensis to cleave urea into ammonia and carbon dioxide allows for a symbiotic relationship with ammonia-oxidizing microorganisms (AOM) that lack this urease-production ability also know as negative AOM. [3] A correlation in environment preferences between Nitrospira species with nxrB gene encoding the β-subunit of nitro-oxidoreductase and AOM species with amoA gene further confirmed this relationship. [7] N. moscoviensis provides ammonia via hydrolysis of urea to these ammonia-oxidizing microorganisms which in turn produce nitrite, the primary energy source of N. moscoviensis. [3] The relationship between ureolytic nitrite-oxidizing bacteria and negative AOM is called reciprocal feeding. [3] Thus far, Nitrospira species have been recognized in natural environments as the primary vehicle for nitrite oxidation including soils, activated-sludge, ocean and fresh water, hot springs, and water treatment plants. [8]
Following its isolation, N. moscoviensis’s genome was sequenced by Dr. Ehrich et al. [2] Its 4.59 Mb genome has a GC content of 56.9+/-0.4 mol% with a predicted 4,863 coding sequences. [2] [3] N. moscoviensis's 16S rRNA gene sequences were found to be 88.9% similar to N. marina’s. [2] Despite its relatively low similarity to N. marina, N. moscoviensis was classified within the Nitrospirae phylum primarily due to shared morphological features including the presence of an enlarged periplasmic space. [2]
N. moscoviensis’s fully sequenced genome has provided useful phylogenetic insights beyond the scope of 16S rRNA sequence studies. [7] The discovery of the gene encoding the β-subunit of nitrite- oxidoreductase, nxrB, from N. moscoviensis as a functional genetic marker of Nitrospira, not only confirmed previous 16S rRNA phylogenetic classifications within the phylum, but revealed a new understanding of Nitrospira’s richness in terrestrial environments. [7] The phylum has expanded from two bacteria, N. marina and N. moscoviensis, to a 6-branched genera composed of a characteristically diverse group of nitrite-oxidizing bacteria with N. moscoviensis positioned in lineage II. [8]
The cytoplasm of Nitrospira moscoviensis contains polyhydroxybutyrate (PHB) granules. [2] PHB granules are polyhydroxyalkanoate (PHA) polymers. [9] PHB granules are produced by N. moscoviensis when the presence of nitrate is limited. [9] When nutrient limitations are no longer present, N. moscoviensis degrades PHB granules using enzymes, and recycling the degraded materials for functional use as a carbon source. [9] Synthetic polymers are used to make most plastics, synthetic polymers are non-biodegradable and contribute negatively to the environment. [9] Unlike synthetic polymers polyhydroxybutyrate is a biopolymer, meaning it can be bio-degraded. [9] PHB can be utilized for packaging, medical purposes like reconstructive surgery, and personal hygiene products. [9]
- ^ Garrity, George; Castenholz, Richard W.; Boone, David R., eds. (2001). Bergey's Manual of Systematic Bacteriology (2nd ed.). New York, NY: New York, NY. pp. 451–453. ISBN 978-0-387-21609-6.
- ^ a b c d e f g h i j k l m n o p q Ehrich, S; Behrens, D; Ludwig, W; Bock, E (1995). "A new obligately chemolithoautotrophic, nitrite-oxidizing bacterium, nitrospira moscoviensis sp. nov. and its phylogenetic relationship". Arch Microbiol. 164 (1): 16–23. doi: 10.1007/BF02568729.
- ^ a b c d e f g h i j k l Koch, H.; Luecker, S.; Albertsen, M.; Kitzinger, K.; Herbold, K.; Spieck, E.; Daims, H. (2015). "Expanded metabolic versatility of ubiquitous nitrite-oxidizing bacteria from the genus nitrospira". Proceedings of the National Academy of Sciences, USA. 112 (36).
- ^ a b Watson, S.W.; Bock, E.; Valois, F.W.; Waterbury, J.B.; Schlosser, U (1986). "Nitrospira marina gen. nov. sp. nov.: a chemolitho- trophic nitrite-oxidizing bacterium". Arch Microbiol. 144 (1): 1–7. doi: 10.1007/BF00454947.
- ^ a b c d e f Spieck, E.; Ehrich, S; Aamand, J; Bock, E. (1998). "Isolation and immunocytochemical location of the nitrite-oxidizing system in nitrospira moscoviensis". Arch Microbiol. 169 (3): 225–230. doi: 10.1007/s002030050565.
- ^ Kirstein, K; Bock, E (1993). "Close genetic relationship between Ni- trobacter hamburgensis nitrite oxidoreductase and Escherichia coli nitrate reductases". Arch Microbiol. 160 (6): 447–453. doi: 10.1007/BF00245305.
- ^ a b c Pester, Michael; Maixner, Frank; Berry, David; Rattei, Thomas; Koch, Hanna; Lücker, Sebastian; Nowka, Boris; Richter, Andreas; Spieck, Eva (2014-10-01). "NxrB encoding the beta subunit of nitrite oxidoreductase as functional and phylogenetic marker for nitrite-oxidizing Nitrospira". Environmental Microbiology. 16 (10): 3055–3071. doi: 10.1111/1462-2920.12300. ISSN 1462-2920.
- ^ a b Nowka, Boris; Off, Sandra; Daims, Holger; Spieck, Eva (2015-03-01). "Improved isolation strategies allowed the phenotypic differentiation of two Nitrospira strains from widespread phylogenetic lineages". FEMS Microbiology Ecology. 91 (3): fiu031. doi: 10.1093/femsec/fiu031. ISSN 1574-6941. PMID 25764560.
- ^ a b c d e f Ojumu, T.V.; Solomon, B.O (2004). "Production of Polyhydroxyalkanoates, a bacterial biodegradable polymer" (PDF). African Journal of Biotechnology. 3 (1): 18–24.