I'm editing Wikipedia as part of this assignment and here's a link to my sandbox
SR proteins are a conserved family of splicing proteins rich in alternating arginine and serine protein residues. SR proteins are important in constitutive and alternative pre-mRNA splicing and some post splicing activities of mRNA. [1] Proteins are classified as SR proteins if they have at least one RNA recognition motif (RRM) and one RS Domain. [1] Other qualification include recognition by the antibody mAb104 and purification using magnesium chloride. [1] In some cases a protein may lack the RRM domain, but is able to interact with RNA through other means. These proteins are called SR-related proteins. [1] SR proteins are localized to the nucleus of cells, specifically in nuclear speckles. [1]
SR proteins were discovered in the 1990's in Drosophila and around the same time in amphibian oocytes. [2] [3] Further investigation has found SR proteins in humans. [1] In general, metazoans, multicellular organisms, appear to have SR proteins and unicellular organisms lack SR proteins. [4]
SR proteins have RNA recognition sites at N- and C-terminus arginine and serine rich sites. [5]
In plants it is thought that SR proteins play a role in development of specific tissues and responses to stress. [5] SR proteins also have a role in regulating post-splicing mRNA metabolism and general mRNA metabolism. [6]
SR proteins also bind to histone 3 tail to participate in transcriptional elongation to further organize gene expression not only at the RNA level, but also at the DNA level. [7]
- ^
a
b
c
d
e
f Long, Jennifer C.; Caceres, Javier F. (2009 Jan 1). "The SR protein family of splicing factors: master regulators of gene expression". The Biochemical Journal. 417 (1): 15–27.
doi:
10.1042/BJ20081501.
PMID
19061484.
{{ cite journal}}: Check date values in:|date=( help)CS1 maint: date and year ( link) -
^ Shepard, Peter J.; Hertel, Klemens J. (2009).
"The SR protein family". Genome Biology. 10 (10): 242.
doi:
10.1186/gb-2009-10-10-242.
PMC
2784316.
PMID
19857271.
{{ cite journal}}: CS1 maint: unflagged free DOI ( link) -
^ Zhong, Xiang-Yang; Wang, Pingping; Han, Joonhee; Rosenfeld, Michael G.; Fu, Xiang-Dong (2009 Jul 10).
"SR proteins in vertical integration of gene expression from transcription to RNA processing to translation". Molecular Cell. 35 (1): 1–10.
doi:
10.1016/j.molcel.2009.06.016.
PMC
2744344.
PMID
19595711.
{{ cite journal}}: Check date values in:|date=( help) -
^ Blencowe, BJ (1999). "SR-related proteins and the processing of messenger RNA precursors". Biochemistry and Cell Biology = Biochimie et Biologie Cellulaire. 77 (4): 277–91.
doi:
10.1139/o99-048.
PMID
10546891.
{{ cite journal}}: Unknown parameter|coauthors=ignored (|author=suggested) ( help) - ^ a b Reddy, Anireddy S. N.; Shad Ali, Gul (1 November 2011). "Plant serine/arginine-rich proteins: roles in precursor messenger RNA splicing, plant development, and stress responses". Wiley Interdisciplinary Reviews: RNA. 2 (6): 875–889. doi: 10.1002/wrna.98. PMID 21766458.
-
^ Shepard, P. J.; Hertel, K. J. (2009).
"The SR protein family". Genome Biology. 10 (10): 242.
doi:
10.1186/gb-2009-10-10-242.
PMC
2784316.
PMID
19857271.
{{ cite journal}}: CS1 maint: unflagged free DOI ( link) - ^ Zhong, Xiang-Yang; Wang, Pingping; Han, Joonhee; Rosenfeld, Michael G.; Fu, Xiang-Dong (2009). "SR Proteins in Vertical Integration of Gene Expression from Transcription to RNA Processing to Translation". Molecular Cell. 35 (1): 1–10. doi: 10.1016/j.molcel.2009.06.016. PMC 2744344. PMID 19595711.
I'm editing Wikipedia as part of this assignment and here's a link to my sandbox
SR proteins are a conserved family of splicing proteins rich in alternating arginine and serine protein residues. SR proteins are important in constitutive and alternative pre-mRNA splicing and some post splicing activities of mRNA. [1] Proteins are classified as SR proteins if they have at least one RNA recognition motif (RRM) and one RS Domain. [1] Other qualification include recognition by the antibody mAb104 and purification using magnesium chloride. [1] In some cases a protein may lack the RRM domain, but is able to interact with RNA through other means. These proteins are called SR-related proteins. [1] SR proteins are localized to the nucleus of cells, specifically in nuclear speckles. [1]
SR proteins were discovered in the 1990's in Drosophila and around the same time in amphibian oocytes. [2] [3] Further investigation has found SR proteins in humans. [1] In general, metazoans, multicellular organisms, appear to have SR proteins and unicellular organisms lack SR proteins. [4]
SR proteins have RNA recognition sites at N- and C-terminus arginine and serine rich sites. [5]
In plants it is thought that SR proteins play a role in development of specific tissues and responses to stress. [5] SR proteins also have a role in regulating post-splicing mRNA metabolism and general mRNA metabolism. [6]
SR proteins also bind to histone 3 tail to participate in transcriptional elongation to further organize gene expression not only at the RNA level, but also at the DNA level. [7]
- ^
a
b
c
d
e
f Long, Jennifer C.; Caceres, Javier F. (2009 Jan 1). "The SR protein family of splicing factors: master regulators of gene expression". The Biochemical Journal. 417 (1): 15–27.
doi:
10.1042/BJ20081501.
PMID
19061484.
{{ cite journal}}: Check date values in:|date=( help)CS1 maint: date and year ( link) -
^ Shepard, Peter J.; Hertel, Klemens J. (2009).
"The SR protein family". Genome Biology. 10 (10): 242.
doi:
10.1186/gb-2009-10-10-242.
PMC
2784316.
PMID
19857271.
{{ cite journal}}: CS1 maint: unflagged free DOI ( link) -
^ Zhong, Xiang-Yang; Wang, Pingping; Han, Joonhee; Rosenfeld, Michael G.; Fu, Xiang-Dong (2009 Jul 10).
"SR proteins in vertical integration of gene expression from transcription to RNA processing to translation". Molecular Cell. 35 (1): 1–10.
doi:
10.1016/j.molcel.2009.06.016.
PMC
2744344.
PMID
19595711.
{{ cite journal}}: Check date values in:|date=( help) -
^ Blencowe, BJ (1999). "SR-related proteins and the processing of messenger RNA precursors". Biochemistry and Cell Biology = Biochimie et Biologie Cellulaire. 77 (4): 277–91.
doi:
10.1139/o99-048.
PMID
10546891.
{{ cite journal}}: Unknown parameter|coauthors=ignored (|author=suggested) ( help) - ^ a b Reddy, Anireddy S. N.; Shad Ali, Gul (1 November 2011). "Plant serine/arginine-rich proteins: roles in precursor messenger RNA splicing, plant development, and stress responses". Wiley Interdisciplinary Reviews: RNA. 2 (6): 875–889. doi: 10.1002/wrna.98. PMID 21766458.
-
^ Shepard, P. J.; Hertel, K. J. (2009).
"The SR protein family". Genome Biology. 10 (10): 242.
doi:
10.1186/gb-2009-10-10-242.
PMC
2784316.
PMID
19857271.
{{ cite journal}}: CS1 maint: unflagged free DOI ( link) - ^ Zhong, Xiang-Yang; Wang, Pingping; Han, Joonhee; Rosenfeld, Michael G.; Fu, Xiang-Dong (2009). "SR Proteins in Vertical Integration of Gene Expression from Transcription to RNA Processing to Translation". Molecular Cell. 35 (1): 1–10. doi: 10.1016/j.molcel.2009.06.016. PMC 2744344. PMID 19595711.