From Wikipedia, the free encyclopedia
Immune electron microscopy is a type of EM used to diagnose some viral infections.
[1]
It is difficult.
[2]
IEM was developed for identifying transmissible gastroenteritis viruses, e.g.
rotavirus in feces.
[3]
IEM is fast and sensitive. IEM relies on antibody-virus complexes.
[4]
Procedure
Electron micrograph of gold nanoparticles attached to
rotaviruses . The small dark circular objects are gold nanoparticles coated with a
monoclonal antibody specific for rotavirus protein VP6.
Was copy/pasted from this ref.
[5]
Application
This was a bit of a hodgepodge:
While IEM is good for recognizing
hepatitis A
antigen , it's challenging to scale up.
In 1973, Feinstone et al. used IEM to find hepatitis A virus in prisoners.
[6]
IEM can detect potato viruses.
[7]
IEM can detect plant viruses.
[8]
References
^
Orthopoxviruses Pathogenic for Humans . 2006-06-09.
ISBN
9780387253060 .
^ Bradley, David E. (1984).
"Characteristics and Function of Thick and Thin Conjugative Pili Determined by Transfer-derepressed Plasmids of Incompatibility Groups It, Ia, Is, B, K and Z" (PDF) . Microbiology . 130 (6): 1489–1502.
doi :
10.1099/00221287-130-6-1489 .
PMID
6148378 . Retrieved 1 October 2018 .
^ Saif, LJ; Bohl, EH; Kohler, EM; Hughes, JH (1977). "Immune electron microscopy of transmissible gastroenteritis virus and rotavirus (reovirus-like agent) of swine". American Journal of Veterinary Research . 38 (1): 13–20.
PMID
189646 .
^ Katz, D; Straussman, Y; Shahar, A; Kohn, A (1980). "Solid-phase immune electron microscopy (SPIEM) for rapid viral diagnosis". Journal of Immunological Methods . 38 (1–2): 171–4.
doi :
10.1016/0022-1759(80)90341-5 .
PMID
7452001 .
^
"Immuno-Electron Microscopy" . umassmed.edu . 2013-11-02. Retrieved 1 October 2018 .
^ Dienstag, Jules L.; Purcell, Robert H. (1977).
"Recent advances in the identification of hepatitis viruses*" (PDF) . Postgraduate Medical Journal . 53 (621): 364–373.
doi :
10.1136/pgmj.53.621.364 . Retrieved 1 October 2018 .
^ Gars, I. D.; Paul Khurana, S. M. (1991). Horticulture — New Technologies and Applications . Current Plant Science and Biotechnology in Agriculture. Vol. 12. pp. 329–336.
doi :
10.1007/978-94-011-3176-6_52 .
ISBN
978-94-010-5401-0 .
^ Shukla, D. D.; Gough, K. H. (1979).
"The Use of Protein A, from Staphylococcus aureus, in Immune Electron Microscopy for Detecting Plant Virus Particles" (PDF) . Journal of General Virology . 45 (2): 533–536.
doi :
10.1099/0022-1317-45-2-533 . Retrieved 1 October 2018 .