 | This is a user sandbox of
AdamCF87. You can use it for testing or practicing edits. This is not the sandbox where you should draft your assigned article for a dashboard.wikiedu.org course. To find the right sandbox for your assignment, visit your Dashboard course page and follow the Sandbox Draft link for your assigned article in the My Articles section. |
Background
Biology3595 is an advanced genetics course at Western University. Aside from coverage of current genetics topics by active researchers in the Biology department at Western University, there is also a Wikipedia component in which students learn how to create and edit Wikipedia articles with the intent of improving genetics related Wikipedia articles. This page is meant to be a training ground for Biology3595 students to improve their understanding of the course and to practice their Wikipedia skills.
This page attracts a growing list of Educational Outcomes for the course.
i) interpret the sorts of data presented in Figures and Tables in current genetics related journal articles
ii) apply the fundamental information and analyses studied in each paper to novel situations.
iii) learn how to create, edit and evaluate a genetics article in Wikipedia
Trying out sandbox and visual editing
Working with Escherichia coli
--Tutorial3--
:notes::
Microarrays
How does it work?
- Purifies mrna-> amplify -> create cdna ->hybridize to chip tat is complementary to this
the higher the expression the higher the signal
use for analysis of differential expression analysis
limitations
specificity; lack thereof
if no knowledge of what to probe of, cannot test for. ie need gene characterization
dna methylation
extract gdna->shear/denature->IP against 5methylC->sodium bisulfite sequencing
--sodium bisulfite sequencing
template w/o sod bi treatment
sodbiseq: convert cyto to uracil
compare to neg control
MeDip - Methylated DNA Immunoprecipitation
Allows provision of score to diff exp analysis using an antibody specific for methylated nucleotides followed by statistical analysis
The higher the score the higher the level of methylation of a seq in a tissue
Determines extent of methylation of gdna in a particular tissue
ChIp CHIP
IP w/ Ab for meth histone (depending on what mark you're looking for)
Gene identification
Sequence candidates -> BLAST/database searching
Droplet digital PCR
PCR type that allows enrichment of low amts of template
gives quantification of presence
| This is a user sandbox of
AdamCF87. You can use it for testing or practicing edits. This is not the sandbox where you should draft your assigned article for a dashboard.wikiedu.org course. To find the right sandbox for your assignment, visit your Dashboard course page and follow the Sandbox Draft link for your assigned article in the My Articles section. |
Background
Biology3595 is an advanced genetics course at Western University. Aside from coverage of current genetics topics by active researchers in the Biology department at Western University, there is also a Wikipedia component in which students learn how to create and edit Wikipedia articles with the intent of improving genetics related Wikipedia articles. This page is meant to be a training ground for Biology3595 students to improve their understanding of the course and to practice their Wikipedia skills.
This page attracts a growing list of Educational Outcomes for the course.
i) interpret the sorts of data presented in Figures and Tables in current genetics related journal articles
ii) apply the fundamental information and analyses studied in each paper to novel situations.
iii) learn how to create, edit and evaluate a genetics article in Wikipedia
Trying out sandbox and visual editing
Working with Escherichia coli
--Tutorial3--
:notes::
Microarrays
How does it work?
- Purifies mrna-> amplify -> create cdna ->hybridize to chip tat is complementary to this
the higher the expression the higher the signal
use for analysis of differential expression analysis
limitations
specificity; lack thereof
if no knowledge of what to probe of, cannot test for. ie need gene characterization
dna methylation
extract gdna->shear/denature->IP against 5methylC->sodium bisulfite sequencing
--sodium bisulfite sequencing
template w/o sod bi treatment
sodbiseq: convert cyto to uracil
compare to neg control
MeDip - Methylated DNA Immunoprecipitation
Allows provision of score to diff exp analysis using an antibody specific for methylated nucleotides followed by statistical analysis
The higher the score the higher the level of methylation of a seq in a tissue
Determines extent of methylation of gdna in a particular tissue
ChIp CHIP
IP w/ Ab for meth histone (depending on what mark you're looking for)
Gene identification
Sequence candidates -> BLAST/database searching
Droplet digital PCR
PCR type that allows enrichment of low amts of template
gives quantification of presence