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![]() | This article was the
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![]() | This article may be too technical for most readers to understand.(September 2010) |
I'm sorry if I'm only complicating this more, but I would add the the rna polymerase moves from 3' prime to 5' prime. Then again, maybe not. :b I just skimmed through the page so I might have skipped it if it was included.
I disagree with the idea that this article is too technical. It really isn't any more or less dense than the related articles on gene expression or molecular biology. I'm leaving the technical notice here on the talk page for now, but am removing the cleanup notice from the article. The edits since October 2005 seem to justify removing the cleanup notice. - DrNixon 23:30, 26 March 2006 (UTC)
Is the section on purification of RNAP necessary? The list is poorly referenced, incomplete, and mainly of interest to the very small number of specialists who might purify RNAP in their research.
I think the very first sentence of this article is somewhat misleading. Firstly, RNA polymerase is not found in all cells! It may also be better to say that RNA polymerases are a family (is there a better word than family for what I'm trying to convey?) of enzymes which synthesise RNA, because the term 'RNA polymerase' on its own is used to mean the prokaryotic RNA polymerase enzyme, and this could cause confusion.
I can't think of the right phrasing to use to make this article both correct and clear at the same time, which is why I'm not going to edit yet. Textbook ref: Mattews, Van Holde and Ahern, "Biochemistry (3rd Ed.)"
Curiousdaughter 14:35, 10 February 2007 (UTC)
Good point. The notion of family is quite correct. As an overview article about polymerases it should make that clear. In addition, the viral RNA dependent RNA polymerases encompass an own family and this should be made clear. CharonZ 15:09, 16 April 2007 (UTC)
There was some info on the C-terminal domain of RNA pol.II in a separate article. I've merged it here before I realized that there actually is a separate article for RNA polymerase II. Should it be moved of there? - tameeria 02:57, 7 May 2007 (UTC)
This article is very thin on apparently important details. Though I realize the whole field is still very new and much probably still is not known.
1. What is the energy source that drives transcription?
2. Apparently loose nucleotide letters must be fairly evenly dispersed in the vicinity of RNAP for transcription to be possible. The process of transcription apparently halts, if the appropriate nucleotide letter needed isn't available to form the RNA chain.
3. Speaking in a purely theoretical sense, if a DNA strand could be an endless loop, would the transcription occur forever? This would be a loop with a single activation zone, so that RNAP can attach and then would loop repeatedly.
DMahalko ( talk) 13:14, 2 November 2011 (UTC)
The page states, "In contrast to DNA polymerase, RNAP includes helicase activity, therefore no separate enzyme is needed to unwind DNA." Yet on the Wikipedia page for Transcription it says the first step is "1.Helicase unwinds/"unzips" the DNA by breaking the hydrogen bonds between complementary nucleotides".
Which is it--does helicase unwind the molecule or RNA polymerase? Or is there some confusion of the terms that would resolve this issue? — Preceding unsigned comment added by 74.51.196.146 ( talk • contribs) 10:05, November 28, 2011
I am looking for contributors to this page to review my revision and expansion to the Lead, as well as all my edits outside of the Lead. I welcome editing, but please no reversions without discussing here first.
Also looking for someone to import a ribbon structure for RNAP; and to expand on the new Structure section I initiated. Regards. IiKkEe ( talk) 15:21, 4 September 2017 (UTC)
I suggest that the misleading term "RNA genes" is removed from this article. In fact I'd go as far as to say it was plain wrong. John2o2o2o ( talk) 10:34, 3 October 2020 (UTC)
![]() | This ![]() It is of interest to the following WikiProjects: | |||||||||||||||||||||||||||||
|
|
![]() | This article was the
MCB Collaboration of the Month for the month of April 2007. For more details, see the
MCB Collaboration of the Month history. |
![]() | This article may be too technical for most readers to understand.(September 2010) |
I'm sorry if I'm only complicating this more, but I would add the the rna polymerase moves from 3' prime to 5' prime. Then again, maybe not. :b I just skimmed through the page so I might have skipped it if it was included.
I disagree with the idea that this article is too technical. It really isn't any more or less dense than the related articles on gene expression or molecular biology. I'm leaving the technical notice here on the talk page for now, but am removing the cleanup notice from the article. The edits since October 2005 seem to justify removing the cleanup notice. - DrNixon 23:30, 26 March 2006 (UTC)
Is the section on purification of RNAP necessary? The list is poorly referenced, incomplete, and mainly of interest to the very small number of specialists who might purify RNAP in their research.
I think the very first sentence of this article is somewhat misleading. Firstly, RNA polymerase is not found in all cells! It may also be better to say that RNA polymerases are a family (is there a better word than family for what I'm trying to convey?) of enzymes which synthesise RNA, because the term 'RNA polymerase' on its own is used to mean the prokaryotic RNA polymerase enzyme, and this could cause confusion.
I can't think of the right phrasing to use to make this article both correct and clear at the same time, which is why I'm not going to edit yet. Textbook ref: Mattews, Van Holde and Ahern, "Biochemistry (3rd Ed.)"
Curiousdaughter 14:35, 10 February 2007 (UTC)
Good point. The notion of family is quite correct. As an overview article about polymerases it should make that clear. In addition, the viral RNA dependent RNA polymerases encompass an own family and this should be made clear. CharonZ 15:09, 16 April 2007 (UTC)
There was some info on the C-terminal domain of RNA pol.II in a separate article. I've merged it here before I realized that there actually is a separate article for RNA polymerase II. Should it be moved of there? - tameeria 02:57, 7 May 2007 (UTC)
This article is very thin on apparently important details. Though I realize the whole field is still very new and much probably still is not known.
1. What is the energy source that drives transcription?
2. Apparently loose nucleotide letters must be fairly evenly dispersed in the vicinity of RNAP for transcription to be possible. The process of transcription apparently halts, if the appropriate nucleotide letter needed isn't available to form the RNA chain.
3. Speaking in a purely theoretical sense, if a DNA strand could be an endless loop, would the transcription occur forever? This would be a loop with a single activation zone, so that RNAP can attach and then would loop repeatedly.
DMahalko ( talk) 13:14, 2 November 2011 (UTC)
The page states, "In contrast to DNA polymerase, RNAP includes helicase activity, therefore no separate enzyme is needed to unwind DNA." Yet on the Wikipedia page for Transcription it says the first step is "1.Helicase unwinds/"unzips" the DNA by breaking the hydrogen bonds between complementary nucleotides".
Which is it--does helicase unwind the molecule or RNA polymerase? Or is there some confusion of the terms that would resolve this issue? — Preceding unsigned comment added by 74.51.196.146 ( talk • contribs) 10:05, November 28, 2011
I am looking for contributors to this page to review my revision and expansion to the Lead, as well as all my edits outside of the Lead. I welcome editing, but please no reversions without discussing here first.
Also looking for someone to import a ribbon structure for RNAP; and to expand on the new Structure section I initiated. Regards. IiKkEe ( talk) 15:21, 4 September 2017 (UTC)
I suggest that the misleading term "RNA genes" is removed from this article. In fact I'd go as far as to say it was plain wrong. John2o2o2o ( talk) 10:34, 3 October 2020 (UTC)