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Why is there a link to a particular company in the sources of the article? There are other companies that offer the same product, so it seems quite biased. — Preceding unsigned comment added by Enbaros ( talk • contribs) 13:59, 21 August 2013 (UTC)
Deleted following passage: "PBS can be used as a diluent in methods to dry biomolecules, as water molecules within it will be structured around the substance (protein, for example) to be 'dried' and immobilized to a solid surface[citation needed]. The thin film of water that binds to the substance prevents denaturation or other conformational changes. Carbonate buffers may be used for the same purpose but with less effectiveness[citation needed]. PBS can be used to take a reference spectrum when measuring the protein adsorption in ellipsometry[citation needed]." This passage is misleading at best. It also very narrowly refers to certain applications taken out of context, which does not make much sense. -- Xenon129 ( talk) 23:35, 28 October 2011 (UTC)
Why is PBS used instead of ordinary saline?
When do you use PBS with calcium and magniesium, and when do you without. When we wash the cells before adding trypsin, we use PBS without magnesium and calcium, but later on we use PBS with calcium and magnesium. why is it so?
Dulbecco's Media and Salts Selection Guide
I used the think PBS was just one thing, but actually there are lots of recipes, this should be reflected in the article (will add it if I have a chance).
is pbs isotonic with human cells because article says its not isotonic and then in application it says its isotonic so plz edit — Preceding unsigned comment added by 27.106.80.242 ( talk) 19:28, 2 November 2013 (UTC)
There appears to be a dispute as to the proper formulation of PBS. The following appears commented out in the article:
THIS RECIPE FOR PBS IS INCORRECT, I HAVE NEVER SEEN A K AND Na ions together in a recipe. Unfortunately, I do not have the time for any editing and correcting of the problem. This person doesn't know what they are talking about. The recipe above refers to Dulbecco’s Phosphate buffered saline without Mg2+, Ca2+. Their suggestion is probably for some derivative of Sorenson or Millonig PBS. It is a completely valid protocol for PBS and IHC applications.
Need more details on the application and introduction sections in phosphate-buffer saline SSASHWIN ( talk) 21:51, 26 January 2017 (UTC) In addition to that, add more information regarding the chemical reactions involving phosphate-buffer saline which will be helpful in understanding more about the topic SSASHWIN ( talk) 21:51, 26 January 2017 (UTC)
I checked the citations, all the links are working perfectly. SSASHWIN ( talk) 21:55, 26 January 2017 (UTC)
"The pH of the 10x stock solution of PBS is ~6.8, but when diluted with water to 1x PBS it should change to 7.4."
This line is incorrect, surely? Buffer pH is not dependent on ion concentration, it should stay the same at 10x dilution.
The 10x stocks you can buy are all pH 7.4. https://www.thermofisher.com/order/catalog/product/AM9625 — Preceding unsigned comment added by 129.11.70.31 ( talk) 15:34, 9 March 2018 (UTC)
" it is good practice to always measure the pH directly using a pH meter. If necessary, pH can be adjusted using hydrochloric acid or sodium hydroxide."
Actually it is far more accurate and reproducible to trust the pH based on weight of the chemicals - consider a 1% error in the weight of one of the phosphates will result in an error of 0.004 pH units; even a 10% error in weighing (inexcusable!) would only make a difference of 0.04 pH units- many glass-electrode pH meters drift more than that during the reading. However it is important to know the purity of the chemicals- dibasic phosphates are deliquescent. CO2 in the water should have an insignificant effect- it affects the pH of unbuffered water but not in the presence of >100 mM phosphate buffer!
Eaberry (
talk) 16:58, 11 January 2024 (UTC)
This article is rated Stub-class on Wikipedia's
content assessment scale. It is of interest to the following WikiProjects: | ||||||||||||||
|
Why is there a link to a particular company in the sources of the article? There are other companies that offer the same product, so it seems quite biased. — Preceding unsigned comment added by Enbaros ( talk • contribs) 13:59, 21 August 2013 (UTC)
Deleted following passage: "PBS can be used as a diluent in methods to dry biomolecules, as water molecules within it will be structured around the substance (protein, for example) to be 'dried' and immobilized to a solid surface[citation needed]. The thin film of water that binds to the substance prevents denaturation or other conformational changes. Carbonate buffers may be used for the same purpose but with less effectiveness[citation needed]. PBS can be used to take a reference spectrum when measuring the protein adsorption in ellipsometry[citation needed]." This passage is misleading at best. It also very narrowly refers to certain applications taken out of context, which does not make much sense. -- Xenon129 ( talk) 23:35, 28 October 2011 (UTC)
Why is PBS used instead of ordinary saline?
When do you use PBS with calcium and magniesium, and when do you without. When we wash the cells before adding trypsin, we use PBS without magnesium and calcium, but later on we use PBS with calcium and magnesium. why is it so?
Dulbecco's Media and Salts Selection Guide
I used the think PBS was just one thing, but actually there are lots of recipes, this should be reflected in the article (will add it if I have a chance).
is pbs isotonic with human cells because article says its not isotonic and then in application it says its isotonic so plz edit — Preceding unsigned comment added by 27.106.80.242 ( talk) 19:28, 2 November 2013 (UTC)
There appears to be a dispute as to the proper formulation of PBS. The following appears commented out in the article:
THIS RECIPE FOR PBS IS INCORRECT, I HAVE NEVER SEEN A K AND Na ions together in a recipe. Unfortunately, I do not have the time for any editing and correcting of the problem. This person doesn't know what they are talking about. The recipe above refers to Dulbecco’s Phosphate buffered saline without Mg2+, Ca2+. Their suggestion is probably for some derivative of Sorenson or Millonig PBS. It is a completely valid protocol for PBS and IHC applications.
Need more details on the application and introduction sections in phosphate-buffer saline SSASHWIN ( talk) 21:51, 26 January 2017 (UTC) In addition to that, add more information regarding the chemical reactions involving phosphate-buffer saline which will be helpful in understanding more about the topic SSASHWIN ( talk) 21:51, 26 January 2017 (UTC)
I checked the citations, all the links are working perfectly. SSASHWIN ( talk) 21:55, 26 January 2017 (UTC)
"The pH of the 10x stock solution of PBS is ~6.8, but when diluted with water to 1x PBS it should change to 7.4."
This line is incorrect, surely? Buffer pH is not dependent on ion concentration, it should stay the same at 10x dilution.
The 10x stocks you can buy are all pH 7.4. https://www.thermofisher.com/order/catalog/product/AM9625 — Preceding unsigned comment added by 129.11.70.31 ( talk) 15:34, 9 March 2018 (UTC)
" it is good practice to always measure the pH directly using a pH meter. If necessary, pH can be adjusted using hydrochloric acid or sodium hydroxide."
Actually it is far more accurate and reproducible to trust the pH based on weight of the chemicals - consider a 1% error in the weight of one of the phosphates will result in an error of 0.004 pH units; even a 10% error in weighing (inexcusable!) would only make a difference of 0.04 pH units- many glass-electrode pH meters drift more than that during the reading. However it is important to know the purity of the chemicals- dibasic phosphates are deliquescent. CO2 in the water should have an insignificant effect- it affects the pH of unbuffered water but not in the presence of >100 mM phosphate buffer!
Eaberry (
talk) 16:58, 11 January 2024 (UTC)