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OK, I'm new to doing anything in Wikipedia bar reading it, so please bear with the newbie!
I've got several points on this page, though lack the skill to do it, and would appreciate others views before I risk learning in depth how to do it!
1) This page talks almost exclusively about restriction endonucleases, and the 'restriction nuclease' link comes through to this page. I would suggest the page is renamed 'Restrction Endonuclease' and a seperate page created to cover the broader 'nucleases' family.
2) The citations for Linn and Arber's two papers on the methylation-restriction system in the late 1960s are:
Linn S., Arber, W. (1968). Host specificity of DNA produced by Escherichia coli, X. In vitro restriction of phage fd replicative form. Proc. Natl. Acad. Sci. USA. 59:1300-1306
Arber, W., Linn S. (1969) DNA modification and restriction. Annu. Rev. Biochem. 38:467-500
3) In the introduction, there is the sentence "One of these enzymes cleaved methylated DNA, while the other cleaved unmethylated DNA at a wide variety of locations along the length of the molecule". I believe this is incorrect- the first enzyme (methylase) adds a methyl group to the DNA, and is linked to an accurate page for 'methylase'.
4) In the 'Site-specific nuclease' section is the phrase 'The term "endonuclease" applies to sequence specific nucleases that break nucleic acid chains somewhere in the interior...'. Internal cutting of the nucleic acid is correct, but endonucleases are not necessarily sequence specific.
Thank you for listening. England Expects 20:10, 28 February 2007 (UTC)
Noticed a problem, but didn't have time to fix it. References 5 - 12 seems to be the same reference. Jajava ( talk) 22:29, 31 August 2018 (UTC)
A picture would be helpful here. Jobonki ( talk) 21:37, 4 December 2014 (UTC)
![]() | This article is rated Start-class on Wikipedia's
content assessment scale. It is of interest to the following WikiProjects: | ||||||||||||||||
|
OK, I'm new to doing anything in Wikipedia bar reading it, so please bear with the newbie!
I've got several points on this page, though lack the skill to do it, and would appreciate others views before I risk learning in depth how to do it!
1) This page talks almost exclusively about restriction endonucleases, and the 'restriction nuclease' link comes through to this page. I would suggest the page is renamed 'Restrction Endonuclease' and a seperate page created to cover the broader 'nucleases' family.
2) The citations for Linn and Arber's two papers on the methylation-restriction system in the late 1960s are:
Linn S., Arber, W. (1968). Host specificity of DNA produced by Escherichia coli, X. In vitro restriction of phage fd replicative form. Proc. Natl. Acad. Sci. USA. 59:1300-1306
Arber, W., Linn S. (1969) DNA modification and restriction. Annu. Rev. Biochem. 38:467-500
3) In the introduction, there is the sentence "One of these enzymes cleaved methylated DNA, while the other cleaved unmethylated DNA at a wide variety of locations along the length of the molecule". I believe this is incorrect- the first enzyme (methylase) adds a methyl group to the DNA, and is linked to an accurate page for 'methylase'.
4) In the 'Site-specific nuclease' section is the phrase 'The term "endonuclease" applies to sequence specific nucleases that break nucleic acid chains somewhere in the interior...'. Internal cutting of the nucleic acid is correct, but endonucleases are not necessarily sequence specific.
Thank you for listening. England Expects 20:10, 28 February 2007 (UTC)
Noticed a problem, but didn't have time to fix it. References 5 - 12 seems to be the same reference. Jajava ( talk) 22:29, 31 August 2018 (UTC)
A picture would be helpful here. Jobonki ( talk) 21:37, 4 December 2014 (UTC)