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The previous form of this page contained a great deal of misinformation, though the author can be forgiven, as there is a great deal of misinformation about this test floating around. I have personal experience in preparing the analytical solutions to perform this test, and was nearly driven crazy trying to divine why nothing I tried gave me a positive test using authentic samples of blood (I was cheap and decided to make my own test kit). It wasn't until I came across a research paper assessing the relative effectiveness of various forensic tests did I see that the phenolphthalein solution was boiled with zinc dust prior to use. I'm really at a loss for why this isn't popularly known, maybe it's some kind of half-assed trade secret on the part of the test kit manufacturers? -- Uberhobo 17:55, 25 August 2007 (UTC)
Thanks to User:Sekiyu for the revisions. I'm not familiar with the name 'phenolphthalin' for the reduced form of phenolphthalein and I'd be cautious in using it as it is important to make the distinction, and yet the names are so very similar. (In the same way that 'hypo-' and 'hyper-' prefixes are disparaged in many medical situations).
Ewen ( talk) 09:42, 29 April 2008 (UTC)
I agree that the similarity between the two names is confusing, but that is the accepted name. Whenever possible, I tried to make the distinction between the reduced -lin form and the oxidized -lein form, and no one wants to read "2-(Bis[4-hydroxyphenyl]methyl)benzoic acid" vs. "3,3-Bis(4-hydroxyphenyl)-1(3H)-isobenzofuranone." Even Sigma Aldrich's chemical entry [1] for it warns against the confusion between the two. Sekiyu ( talk) 15:28, 29 April 2008 (UTC)
What if a strong base was added to the blood sample (such as cleaning out a bathtub with very basic drain cleaner), wouldn't this cause the Phenolphthalein to change color also? 64.180.188.6 ( talk) 20:25, 26 October 2008 (UTC)
This article formerly contained errors, in the limitations section.
The cox study found that NONE of the tested substances caused false positives with KM
False positives were only found with the orthotolidine and tetramethylbenzidine tests in cox's study.... he even concludes that KM and LMG are two of the most specific tests!! â Preceding unsigned comment added by 82.42.246.140 ( talk) 15:59, 24 February 2013 (UTC)
Sorry, i deleted the false information, but here it is:
http://www.ncbi.nlm.nih.gov/pubmed/1955838 /
http://projects.nfstc.org/workshops/resources/articles/A%20Study%20of%20the%20Sensitivity%20and%20Specificity%20of%20Four.pdf
it used to be cited as a reference saying that all sorts of things caused false positives with KM.. which is the complete opposite of what the study found 82.42.246.140 ( talk) 20:31, 26 February 2013 (UTC)
This article is rated Start-class on Wikipedia's
content assessment scale. It is of interest to the following WikiProjects: | |||||||||||||||||||||||||||||||
|
The previous form of this page contained a great deal of misinformation, though the author can be forgiven, as there is a great deal of misinformation about this test floating around. I have personal experience in preparing the analytical solutions to perform this test, and was nearly driven crazy trying to divine why nothing I tried gave me a positive test using authentic samples of blood (I was cheap and decided to make my own test kit). It wasn't until I came across a research paper assessing the relative effectiveness of various forensic tests did I see that the phenolphthalein solution was boiled with zinc dust prior to use. I'm really at a loss for why this isn't popularly known, maybe it's some kind of half-assed trade secret on the part of the test kit manufacturers? -- Uberhobo 17:55, 25 August 2007 (UTC)
Thanks to User:Sekiyu for the revisions. I'm not familiar with the name 'phenolphthalin' for the reduced form of phenolphthalein and I'd be cautious in using it as it is important to make the distinction, and yet the names are so very similar. (In the same way that 'hypo-' and 'hyper-' prefixes are disparaged in many medical situations).
Ewen ( talk) 09:42, 29 April 2008 (UTC)
I agree that the similarity between the two names is confusing, but that is the accepted name. Whenever possible, I tried to make the distinction between the reduced -lin form and the oxidized -lein form, and no one wants to read "2-(Bis[4-hydroxyphenyl]methyl)benzoic acid" vs. "3,3-Bis(4-hydroxyphenyl)-1(3H)-isobenzofuranone." Even Sigma Aldrich's chemical entry [1] for it warns against the confusion between the two. Sekiyu ( talk) 15:28, 29 April 2008 (UTC)
What if a strong base was added to the blood sample (such as cleaning out a bathtub with very basic drain cleaner), wouldn't this cause the Phenolphthalein to change color also? 64.180.188.6 ( talk) 20:25, 26 October 2008 (UTC)
This article formerly contained errors, in the limitations section.
The cox study found that NONE of the tested substances caused false positives with KM
False positives were only found with the orthotolidine and tetramethylbenzidine tests in cox's study.... he even concludes that KM and LMG are two of the most specific tests!! â Preceding unsigned comment added by 82.42.246.140 ( talk) 15:59, 24 February 2013 (UTC)
Sorry, i deleted the false information, but here it is:
http://www.ncbi.nlm.nih.gov/pubmed/1955838 /
http://projects.nfstc.org/workshops/resources/articles/A%20Study%20of%20the%20Sensitivity%20and%20Specificity%20of%20Four.pdf
it used to be cited as a reference saying that all sorts of things caused false positives with KM.. which is the complete opposite of what the study found 82.42.246.140 ( talk) 20:31, 26 February 2013 (UTC)