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The first paragraph in the 'Basic Steps' is a complete mess and makes no sense! The rest is correct.
74.60.93.26 ( talk) 18:03, 1 October 2009 (UTC)
The claim that gateway cloning has replaced endonucleases and ligases is dubious. Most academic labs still use restriction endonucleases and ligases. This seems to be more of a marketing claim than a statement of fact regarding the frequency of gateway cloning usage in the molecular/cellular biology community. Other techniques like
Gibson Assembly or
Golden Gate Assembly are closer to replacing endonuclease and ligase cloning than is Gateway cloning.
Atlantisiron (
talk)
22:09, 20 July 2011 (UTC)
The article mentions "Invitrogen terminology". But Invitrogen no longer exists. Should this be reworded or should all mention of specific terminology removed? I specifically refer to sections such as " (special Invitrogen nomenclature)." Also, the article could need some visual makeup ... it's not easy to read right now. 84.112.136.52 ( talk) 18:25, 14 October 2013 (UTC)
Briancady413 ( talk) 20:43, 19 January 2016 (UTC)
Hi, my name is Buse. I'm a Molecular Biology master student and editing the page was given to me as an assignment for Recent Developments in Biotechnology (MLC501) in Uskudar University.
outreachdashboard.wmflabs.org/courses/Uskudar_University,_Istanbul,_Turkiye/MLC501_Recent_Developments_in_Biotechnology_2022-2023_Fall_(2022-2023)/home Buse Baran ( talk) 10:05, 26 January 2023 (UTC)
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| This article is rated Start-class on Wikipedia's
content assessment scale. It is of interest to the following WikiProjects: | |||||||||||||
| ||||||||||||||
The first paragraph in the 'Basic Steps' is a complete mess and makes no sense! The rest is correct.
74.60.93.26 ( talk) 18:03, 1 October 2009 (UTC)
The claim that gateway cloning has replaced endonucleases and ligases is dubious. Most academic labs still use restriction endonucleases and ligases. This seems to be more of a marketing claim than a statement of fact regarding the frequency of gateway cloning usage in the molecular/cellular biology community. Other techniques like
Gibson Assembly or
Golden Gate Assembly are closer to replacing endonuclease and ligase cloning than is Gateway cloning.
Atlantisiron (
talk)
22:09, 20 July 2011 (UTC)
The article mentions "Invitrogen terminology". But Invitrogen no longer exists. Should this be reworded or should all mention of specific terminology removed? I specifically refer to sections such as " (special Invitrogen nomenclature)." Also, the article could need some visual makeup ... it's not easy to read right now. 84.112.136.52 ( talk) 18:25, 14 October 2013 (UTC)
Briancady413 ( talk) 20:43, 19 January 2016 (UTC)
Hi, my name is Buse. I'm a Molecular Biology master student and editing the page was given to me as an assignment for Recent Developments in Biotechnology (MLC501) in Uskudar University.
outreachdashboard.wmflabs.org/courses/Uskudar_University,_Istanbul,_Turkiye/MLC501_Recent_Developments_in_Biotechnology_2022-2023_Fall_(2022-2023)/home Buse Baran ( talk) 10:05, 26 January 2023 (UTC)