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This article has been selected for improvement to complete a class project for the Johns Hopkins University 410.602 Molecular Biology Course. Main topics for construction include the following:
These topics will be expanded upon based on reliable sources, and images will also be utilized to improve the quality of this article. Tisquestra ( talk) 17:24, 13 March 2013 (UTC)
Concerning the images in this article, some may be redundant or unnecessary. I have reduced the size and it looks better than before. Suggestions? Jberendt ( talk) 01:58, 5 April 2013 (UTC)
My only comment is that since there are so many acronyms and abbreviations, a consistent approach should be used. The Style Guidelines state that "[i]f the subject of the page has a common abbreviation or more than one name, the abbreviation (in parentheses) and each additional name should be in boldface on its first appearance." The example cited is: Sodium hydroxide (NaOH), also known as lye and caustic soda, is ...
Based on a review of the citations, I verified support for all the main assertions. Most important from a fact checking perspective are the dates in the history section. The technical description appears accurate. That being said, there is a discussion at the end assessing the advantages and disadvantages which, to the extent it is further developed, should be careful about neutrality and should otherwise cite to sources which support any claim.
Since the post is directed to labeling techniques, the authors have done a good job of keeping the description straight forward and citing to literature that provides a thorough but clear explanation. It is perhaps inevitable that most of it is on the technical side, however, this is balanced by the tone of the article.
The contents of each section all appear to belong in that section. There are many headers that have not been completed as of this review and perhaps instead of separate headers they could be incorporated into each other. For example, it may be better to clarify the distinction between "Methods and Various Types of Tags" and "Fluorescent Labeling Techniques" as separate headers. To the extent there is overlap, maybe this could all be one section.
If there is a way to explain plainly upfront the general process of how a fluorescent tag is attached, i.e., chemically, that may be helpful as a segue into the more technical discussion of the different types and techniques. That is only out of consideration of the fact that if you did not know anything about this subject, that may make it more accessible right off the bat before drilling down into the nuts and bolts of the individual techniques.
I did not observe anything in particular that looks ambiguous. There are some very good primers on the history and the development of the techniques. Generally, when referring to "biotech" it is unclear what that means. So I would suggest removing reference to that term, at least in abbreviated form. To the extent these techniques have been widely adopted by researches and developers of biochemical assays, you may want to be more specific or else cite to something that refers to biotechnology as a sector. That is not inaccuracy but may need clarification.
As mentioned, it may be helpful to clarify the distinction between "Methods and Various Types of Tags" and "Fluorescent Labeling Techniques" as separate headers. To the extent there is overlap, maybe this could all be one section. Overall my recommendation is that the headers should correspond to discrete concepts. Currently there are different headers for chemicals used, methods used to attach them, different types of sensing, but they all seem to be somewhat related. It may be worth rethinking the organization and removing some of the headers.
One thought is that since labeling is a technique, it easily turns into a more specialized and technical or niche topic. It is something scientists use rather than study. So to the extent that people have developed labeling to help identify, measure, or otherwise detect something at the molecular level maybe providing a few concrete examples of what the research applications are, e.g., why would you want to detect and/or determine a quantity of protein, would be helpful.
Yes! Great job so far. Keep up the good work.
Ppelletier ( talk) 21:31, 12 April 2013 (UTC)
Klortho ( talk) 02:56, 26 April 2013 (UTC)
This is a good article with a lot of relative information in it. I think it is structured properly but I do agree with some of the threads already on this page that the inconsistent switching between label and tag can be a little confusing. I would suggest changing everything to tag to match the article. The other issue that was somewhat confusing was the 'Methods' section followed by the 'Techniques' section. It seemed like there should be a marked difference between the sections, but that difference was a bit ambiguous. If you concentrated on the physical tags in the 'Types of tags' section and moved what methods material there was to the techniques section I think it might improve the flow. It's mostly there, the 'Fluorescent labels can be hybridized to mRNA...' is a pretty good example. Some other points:
There are good references, quality and number. And the images are great, they really add to the visual interest of the article. If you're looking for more information on recent discoveries (its in my head because it was part of this weeks discussion), here's a paper on GFP used in fate mapping in mice:
{{
cite journal}}
: Explicit use of et al. in: |author=
(
help); Unknown parameter |month=
ignored (
help)CS1 maint: multiple names: authors list (
link).Overall a great job! Dugalmaguire ( talk) 00:21, 2 May 2013 (UTC)
The lead section is a great overview of the article topic which includes frequently used techniques and common tags. Without going into too much detail, this section conveys the notability of fluorescent tagging so I don’t think much if anything needs to be added to it. The image is also a great addition!
I think one of the main issues, as has been discussed, is distinguishing or remaining consistent with the terms “tagging” and “labeling”. If they are synonymous, then I think that it should be mentioned in the lead section (i.e. In molecular biology and biotechnology, a fluorescent tag or label….). If they aren’t synonymous then I think it would help the reader greatly if this distinction was made in this section. Either label(ing) or tag(ging) should then be used throughout the remainder of the article. For example, the third section is titled “Fluorescent tagging techniques” but is followed by a paragraph that starts with “Fluorescent labeling is known for…..”.
I agree with dugalmaguire that the difference between “Methods and various types of tags” and “Fluorescent tagging techniques” is a bit unclear. It seems as if the former section is about ways in which tagged biomolecules can be visualized, while the latter is about how these biomolecules are tagged. If I have a correct understanding about the nature of the two sections, then I think it would be helpful if the section titled “Methods and various types of tags” is changed to “Methods of visualizing biomolecules”, alleviating some confusion between the two sections. In addition, it would be helpful to include a short summary (1 or 2 sentences) in the “Methods and various types of tags” section to give a general overview/brief summary of the range of biomolecule tracking.
It seems as if the “Recent discoveries” section would be more appropriately named “Recent advancements in cell imaging” or something along those lines. A bit more content could be added to this section. It seems as if Halo-tag is an important advancement in in vivo imaging that is worth expounding upon.
The HaloTag: Improving Soluble Expression and Applications in Protein Functional Analysis (Curr Chem Genomics. 2012;6:8-17. doi: 10.2174/1875397301206010008. Epub 2012 Sep 20.), provides general information about this technology.
Making the message clear: visualizing mRNA localization (Trends Cell Biol. 2010 Jul;20(7):380-90. doi: 10.1016/j.tcb.2010.03.006. Epub 2010 May 3.), provides a great deal of information about live-cell imaging of mRNA localization which serves as a great addition to the last paragraph, “Advantages”.
Overall, the article is developing nicely with the main concern being consistency with terminology and expansion on information in the last two sections.
Nguych01 (
talk)
02:24, 2 May 2013 (UTC)
According to the descriptions here, they are the same, and the two terms can be used interchangeably. The description of the process on that page backs this up; it says, "Fluorescent labelling is the process of covalently attaching a fluorophore to another molecule ...". So, let's merge them. Any discussion? Klortho ( talk) 11:45, 13 May 2013 (UTC)
Overall I think that the improvements to the initial article are good. Most of the statements had good references. There were a few places where a general statement about the subject was made with no supporting information, but then that statement was referenced in a later paragraph. An example of this is the area where the beginnings and "firsts" of fluorescence were mentioned. There is a paragraph with references, but the last sentence of the paragraph before that was not cited in anyway.
The contributors did a good job of trying to not make the article too technical which can be difficult with a topic such as this. There were a few areas where technical words were used without any explanation, but again this may have been necessary due to the subject matter.
The organization of the article works well with separate sections and subsections for each of the topics. There was also decent amount of information for each of the subsections. A few of the pictures that went with the subsections may not have been necessary. Although they are interesting to look at, without more explanation as to their significance, they don't add to the understanding of the subject matter. Jbmcgregor ( talk) 21:12, 8 July 2013 (UTC)
This article is rated C-class on Wikipedia's
content assessment scale. It is of interest to the following WikiProjects: | ||||||||||||||
|
This article has been selected for improvement to complete a class project for the Johns Hopkins University 410.602 Molecular Biology Course. Main topics for construction include the following:
These topics will be expanded upon based on reliable sources, and images will also be utilized to improve the quality of this article. Tisquestra ( talk) 17:24, 13 March 2013 (UTC)
Concerning the images in this article, some may be redundant or unnecessary. I have reduced the size and it looks better than before. Suggestions? Jberendt ( talk) 01:58, 5 April 2013 (UTC)
My only comment is that since there are so many acronyms and abbreviations, a consistent approach should be used. The Style Guidelines state that "[i]f the subject of the page has a common abbreviation or more than one name, the abbreviation (in parentheses) and each additional name should be in boldface on its first appearance." The example cited is: Sodium hydroxide (NaOH), also known as lye and caustic soda, is ...
Based on a review of the citations, I verified support for all the main assertions. Most important from a fact checking perspective are the dates in the history section. The technical description appears accurate. That being said, there is a discussion at the end assessing the advantages and disadvantages which, to the extent it is further developed, should be careful about neutrality and should otherwise cite to sources which support any claim.
Since the post is directed to labeling techniques, the authors have done a good job of keeping the description straight forward and citing to literature that provides a thorough but clear explanation. It is perhaps inevitable that most of it is on the technical side, however, this is balanced by the tone of the article.
The contents of each section all appear to belong in that section. There are many headers that have not been completed as of this review and perhaps instead of separate headers they could be incorporated into each other. For example, it may be better to clarify the distinction between "Methods and Various Types of Tags" and "Fluorescent Labeling Techniques" as separate headers. To the extent there is overlap, maybe this could all be one section.
If there is a way to explain plainly upfront the general process of how a fluorescent tag is attached, i.e., chemically, that may be helpful as a segue into the more technical discussion of the different types and techniques. That is only out of consideration of the fact that if you did not know anything about this subject, that may make it more accessible right off the bat before drilling down into the nuts and bolts of the individual techniques.
I did not observe anything in particular that looks ambiguous. There are some very good primers on the history and the development of the techniques. Generally, when referring to "biotech" it is unclear what that means. So I would suggest removing reference to that term, at least in abbreviated form. To the extent these techniques have been widely adopted by researches and developers of biochemical assays, you may want to be more specific or else cite to something that refers to biotechnology as a sector. That is not inaccuracy but may need clarification.
As mentioned, it may be helpful to clarify the distinction between "Methods and Various Types of Tags" and "Fluorescent Labeling Techniques" as separate headers. To the extent there is overlap, maybe this could all be one section. Overall my recommendation is that the headers should correspond to discrete concepts. Currently there are different headers for chemicals used, methods used to attach them, different types of sensing, but they all seem to be somewhat related. It may be worth rethinking the organization and removing some of the headers.
One thought is that since labeling is a technique, it easily turns into a more specialized and technical or niche topic. It is something scientists use rather than study. So to the extent that people have developed labeling to help identify, measure, or otherwise detect something at the molecular level maybe providing a few concrete examples of what the research applications are, e.g., why would you want to detect and/or determine a quantity of protein, would be helpful.
Yes! Great job so far. Keep up the good work.
Ppelletier ( talk) 21:31, 12 April 2013 (UTC)
Klortho ( talk) 02:56, 26 April 2013 (UTC)
This is a good article with a lot of relative information in it. I think it is structured properly but I do agree with some of the threads already on this page that the inconsistent switching between label and tag can be a little confusing. I would suggest changing everything to tag to match the article. The other issue that was somewhat confusing was the 'Methods' section followed by the 'Techniques' section. It seemed like there should be a marked difference between the sections, but that difference was a bit ambiguous. If you concentrated on the physical tags in the 'Types of tags' section and moved what methods material there was to the techniques section I think it might improve the flow. It's mostly there, the 'Fluorescent labels can be hybridized to mRNA...' is a pretty good example. Some other points:
There are good references, quality and number. And the images are great, they really add to the visual interest of the article. If you're looking for more information on recent discoveries (its in my head because it was part of this weeks discussion), here's a paper on GFP used in fate mapping in mice:
{{
cite journal}}
: Explicit use of et al. in: |author=
(
help); Unknown parameter |month=
ignored (
help)CS1 maint: multiple names: authors list (
link).Overall a great job! Dugalmaguire ( talk) 00:21, 2 May 2013 (UTC)
The lead section is a great overview of the article topic which includes frequently used techniques and common tags. Without going into too much detail, this section conveys the notability of fluorescent tagging so I don’t think much if anything needs to be added to it. The image is also a great addition!
I think one of the main issues, as has been discussed, is distinguishing or remaining consistent with the terms “tagging” and “labeling”. If they are synonymous, then I think that it should be mentioned in the lead section (i.e. In molecular biology and biotechnology, a fluorescent tag or label….). If they aren’t synonymous then I think it would help the reader greatly if this distinction was made in this section. Either label(ing) or tag(ging) should then be used throughout the remainder of the article. For example, the third section is titled “Fluorescent tagging techniques” but is followed by a paragraph that starts with “Fluorescent labeling is known for…..”.
I agree with dugalmaguire that the difference between “Methods and various types of tags” and “Fluorescent tagging techniques” is a bit unclear. It seems as if the former section is about ways in which tagged biomolecules can be visualized, while the latter is about how these biomolecules are tagged. If I have a correct understanding about the nature of the two sections, then I think it would be helpful if the section titled “Methods and various types of tags” is changed to “Methods of visualizing biomolecules”, alleviating some confusion between the two sections. In addition, it would be helpful to include a short summary (1 or 2 sentences) in the “Methods and various types of tags” section to give a general overview/brief summary of the range of biomolecule tracking.
It seems as if the “Recent discoveries” section would be more appropriately named “Recent advancements in cell imaging” or something along those lines. A bit more content could be added to this section. It seems as if Halo-tag is an important advancement in in vivo imaging that is worth expounding upon.
The HaloTag: Improving Soluble Expression and Applications in Protein Functional Analysis (Curr Chem Genomics. 2012;6:8-17. doi: 10.2174/1875397301206010008. Epub 2012 Sep 20.), provides general information about this technology.
Making the message clear: visualizing mRNA localization (Trends Cell Biol. 2010 Jul;20(7):380-90. doi: 10.1016/j.tcb.2010.03.006. Epub 2010 May 3.), provides a great deal of information about live-cell imaging of mRNA localization which serves as a great addition to the last paragraph, “Advantages”.
Overall, the article is developing nicely with the main concern being consistency with terminology and expansion on information in the last two sections.
Nguych01 (
talk)
02:24, 2 May 2013 (UTC)
According to the descriptions here, they are the same, and the two terms can be used interchangeably. The description of the process on that page backs this up; it says, "Fluorescent labelling is the process of covalently attaching a fluorophore to another molecule ...". So, let's merge them. Any discussion? Klortho ( talk) 11:45, 13 May 2013 (UTC)
Overall I think that the improvements to the initial article are good. Most of the statements had good references. There were a few places where a general statement about the subject was made with no supporting information, but then that statement was referenced in a later paragraph. An example of this is the area where the beginnings and "firsts" of fluorescence were mentioned. There is a paragraph with references, but the last sentence of the paragraph before that was not cited in anyway.
The contributors did a good job of trying to not make the article too technical which can be difficult with a topic such as this. There were a few areas where technical words were used without any explanation, but again this may have been necessary due to the subject matter.
The organization of the article works well with separate sections and subsections for each of the topics. There was also decent amount of information for each of the subsections. A few of the pictures that went with the subsections may not have been necessary. Although they are interesting to look at, without more explanation as to their significance, they don't add to the understanding of the subject matter. Jbmcgregor ( talk) 21:12, 8 July 2013 (UTC)