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Although this term is used much less frequently than far-western blotting its appearance in the literature probably warrants this article. That said, the original attribution of Eastern blotting being developed in 2009 is absurd. The method has been around in various forms since at least the late '70s. If anyone can find earlier references, please add them. -- Glycoform ( talk) 00:24, 10 June 2009 (UTC)
Do not try to modify Eastern Blotting. The Japanese papers mentioning Eastern blotting are Far Eastern Blotting. Do not insert lectin blotting or any other fancy names as it is confusing to the reader. —Preceding unsigned comment added by 70.241.118.175 ( talk) 03:41, 10 June 2009 (UTC)
The relevant passage is on Eastern Blotting and not Lectin blotting. This is not about lectin blotting, if you need your own page create it. Do not try to vandalise other pages. —Preceding unsigned comment added by 129.109.98.97 ( talk) 14:13, 10 June 2009 (UTC)
Please do not vandalise the section of Eastern Blotting. I suggest you create new pages if you are interested. —Preceding unsigned comment added by 129.109.98.97 ( talk) 14:23, 10 June 2009 (UTC)
I am unsure why there is a need to remove cited material without explanation. It may be related to the discussion above regarding lectin binding, but it isn't clear since no reason has been given. I recommend centralized discussion here. Any further reversions that removes sourced information will result in a posting to WP:ANI. Wisdom89 ( T / C) 23:48, 14 June 2009 (UTC)
The article is about Eastern Blotting and not lectin blotting. Never did the earlier papers mentioned in your article use the term Eastern Blotting or detection of post translational protein modifications. Eastern blotting is the detection of protein modifications (not just one modification as mentioned in your statement) of a known protein. The authors of the 70's paper never mentioned the term Eastern Blotting (if yes, correct me). Thus Eastern blotting is detection of protein modifications and not just one modification. The paper on Ehrlichia mentions antigenic protein modfications in two species- I believe this is the first paper comparing protein modifications in two species and using multiple substrates (not just lectins). Thus I justify that the paper by Thomas et al., should be given the credit of first mentioning the term Eastern Blotting. —Preceding
unsigned comment added by
70.241.90.85 (
talk)
04:32, 15 June 2009 (UTC)
Though Kornberg described the DNA polymerase, he did not find any practical applications for it. The name PCR (Polymerase Chain Reaction) (the practical technique of amplifying DNA) was described by Kary Mullis. Hence Kary Mullis is considered the inventor of PCR, not Kornberg who described the DNA polymerase the key ingredient of PCR. Similarly, lectins, Cholera Toxin B etc., were described by various scientists. None of them coined or used the term "Eastern Blotting" until S. Thomas described a practical way of detecting post translational protein modifications. —Preceding unsigned comment added by 129.109.98.97 ( talk) 14:39, 15 June 2009 (UTC)
From Wikipedia: Mullis was not the first to propose the ideas behind PCR. The main principles were described in 1971 by 1968 Nobel Prize laureate H. Gobind Khorana and Kjell Kleppe, a Norwegian scientist. Kleppe and Khorana released a 20-page research paper on PCR in the 1971 Journal of Molecular Biology. As early as June 18, 1969, Kleppe had presented his work at the Gordon Conference in New Hampshire. Using repair replication (the principle of PCR), he duplicated and then quadrupled a small synthetic molecule with the help of two primers and DNA-polymerase. However, "the full potential of PCR was not realized" until Mullis' work in 1983.
Eastern Blotting was not named until the paper of S. Thomas describing detection of post translational protein modifications. It is just not lectin blotting alone. There are 25 post-translational protein modifications and over the years various substrates have been used to detect different moities (including phosphorylation, lipoylation etc) with different substrates. None of them coined the term "Eastern Blotting". Techniques detecting post translational modifications is Eastern Blotting. The article is written for "Eastern Blotting", not any other blotting techniques. —Preceding unsigned comment added by 129.109.98.97 ( talk) 21:38, 16 June 2009 (UTC)
This article is about Eastern Blotting. Only references mentioning the technique of "Eastern Blotting" (detection of post translational protein modification) are to be included in this article. —Preceding unsigned comment added by 129.109.98.107 ( talk) 14:50, 17 June 2009 (UTC)
The lectin blotting reference mentioned by Glycoform: Tanner and Anstee (1976) is not a blot study. The paper never mentions blot. The assay used by Tanner and Anstee is "gel radiolabelled lectin assay". The authors never even showed the radiolabelled gel (which I believe could be shown on an X-ray film) on that paper, but marked the distance calculated as a "graph" (which no scientific paper now consider as legitimate). The first blotting technique Southern Blotting was described in 1975 and Western blotting in 1979!!! —Preceding unsigned comment added by 129.109.98.97 ( talk) 15:22, 17 June 2009 (UTC)
I have taken the liberty of restoring the version that gives ample and even credit to both the naming convention, as well as the pivotal back-work for the application.
Wisdom89 (
T /
C)
16:13, 17 June 2009 (UTC)
Southern Blotting was published in Nov. 1975 (the first blotting technique). Tanner and Anstee send their paper on lectin to Biochemical Journal in 1975 (before the Southern Blotting was published)!!!! Tanner and Anstee never used a blot!!!!! It makes no sense why their name and paper is included as a reference in Eastern Blotting. —Preceding unsigned comment added by 129.109.98.97 ( talk) 16:48, 17 June 2009 (UTC)
What is Far-Eastern blot and In gel lectin assay doing in Eastern Blotting Section? Not good Editors!!!!! —Preceding unsigned comment added by 70.241.98.153 ( talk) 04:03, 18 June 2009 (UTC)
Who coined the term Eastern Blotting (the detection of post translational protein modification)? —Preceding unsigned comment added by 70.241.111.107 ( talk) 20:15, 18 June 2009 (UTC)
I presume the Editors do not read papers carefully: (The term "Eastern blotting" is used only rarely in primary literature, and has appeared more in recent publications (Ishikawa D and Taki T,1998). Even Ishikawa and Taki will be laughing at this wrong quote. Ishikawa and Taki developed the Far-Eastern Blotting (not Eastern Blotting). By the way, if you people do not know what is Far-Eastern Blotting, kindly take some time to read it. —Preceding unsigned comment added by 129.109.98.97 ( talk) 23:48, 18 June 2009 (UTC)
“The technique of Far-Eastern blotting was developed by Ishikawa and Taki (1998) as a method for transferring lipids from an HPTLC plate to a PVDF membrane and later probed with antibodies. The technique was also used by Fukuda et al. (2006) to separate ginsenosides by TLC and blotted to a PVDF membrane treated with NaIO4 solution followed by bovine serum albumin (BSA) which resulted in a ginsenoside–BSA conjugate on the PVDF membrane. The blotted spots were finally stained by antiginsenoside monoclonal antibodies. Though the technique used is the same Far-Eastern technique described by Ishikawa and Taki (1998) the authors used the term Eastern blot for their studies!!!” (Text quoted from- Thomas et al., 2009)
A summary of more recent improvements of the protocol has been provided by H. Freeze (1993)- It is 16 years- still you call it RECENT. —Preceding unsigned comment added by 129.109.98.97 ( talk) 23:35, 29 June 2009 (UTC)
{{
editprotected}}
Administrators:
this version of the page appears to be the version generally accepted by established editors at this time. Recognizing that this may change as the discussion progresses, please revert this article to the preferred version. Editors: if I picked the wrong revision, please correct me.
Zab
Milenko
How am I driving?
15:11, 15 June 2009 (UTC)
As Rootology has announced a wikibreak, and per the discussion above, I have reduced the protection to semi. To the unregistered user: please discuss your changes on this page first and then add {{ editsemiprotected}} if a consensus develops. Regards, — Martin ( MSGJ · talk) 22:15, 15 June 2009 (UTC)
Based on the controversy here, I did some digging in the literature. I was able to find no fewer than five papers that claim to have named the Eastern blot. Many of these are quite similar, and not just new applications of a technique that they use the term for. I tried to include only papers that had specific text stating that this was a new technique (some even put it in the title of the paper.) I think the article needs to acknowledge all of these, and should probably reflect the lack of consensus in the primary literature. It may also be worth re-assessing which articles to highlight as applications. To be fair, all versions of the term should have some examples, but this makes for a large addition of text.Any other examples welcome, especially any that predate those included already.-- Glycoform ( talk) 22:35, 27 June 2009 (UTC)
I have to take issue with this edit; the paragraph in question is heavily referenced, and contains several indisputable facts. For example, the interview and comment by Ed Southern (and paper which was commented on), as well as the appearance of the term in textbooks. Both are entirely relevant to this article. If you have issue with the limited context provided (i.e. pointing out that the referenced article is inconsistent with a second referenced article), I could understand editing it. (Although these two authors are in clear conflict with each other - this is not opinion). As I see it, this edit deletes relevant, and referenced, information from the article. I don't agree that its "slanted" in any way, but if you care to revise to unslant it, please do so without losing other relevant content.-- Glycoform ( talk) 00:32, 16 July 2010 (UTC)
An Eastern Blot is when you run a Western with the polarity backward so all your protein runs off the gel immediately. At least 5 people I know use the term this way, although most of us only know of this Eastern as legend and not from personal experience ;). -- Chinasaur ( talk) 01:15, 3 March 2011 (UTC)
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Although this term is used much less frequently than far-western blotting its appearance in the literature probably warrants this article. That said, the original attribution of Eastern blotting being developed in 2009 is absurd. The method has been around in various forms since at least the late '70s. If anyone can find earlier references, please add them. -- Glycoform ( talk) 00:24, 10 June 2009 (UTC)
Do not try to modify Eastern Blotting. The Japanese papers mentioning Eastern blotting are Far Eastern Blotting. Do not insert lectin blotting or any other fancy names as it is confusing to the reader. —Preceding unsigned comment added by 70.241.118.175 ( talk) 03:41, 10 June 2009 (UTC)
The relevant passage is on Eastern Blotting and not Lectin blotting. This is not about lectin blotting, if you need your own page create it. Do not try to vandalise other pages. —Preceding unsigned comment added by 129.109.98.97 ( talk) 14:13, 10 June 2009 (UTC)
Please do not vandalise the section of Eastern Blotting. I suggest you create new pages if you are interested. —Preceding unsigned comment added by 129.109.98.97 ( talk) 14:23, 10 June 2009 (UTC)
I am unsure why there is a need to remove cited material without explanation. It may be related to the discussion above regarding lectin binding, but it isn't clear since no reason has been given. I recommend centralized discussion here. Any further reversions that removes sourced information will result in a posting to WP:ANI. Wisdom89 ( T / C) 23:48, 14 June 2009 (UTC)
The article is about Eastern Blotting and not lectin blotting. Never did the earlier papers mentioned in your article use the term Eastern Blotting or detection of post translational protein modifications. Eastern blotting is the detection of protein modifications (not just one modification as mentioned in your statement) of a known protein. The authors of the 70's paper never mentioned the term Eastern Blotting (if yes, correct me). Thus Eastern blotting is detection of protein modifications and not just one modification. The paper on Ehrlichia mentions antigenic protein modfications in two species- I believe this is the first paper comparing protein modifications in two species and using multiple substrates (not just lectins). Thus I justify that the paper by Thomas et al., should be given the credit of first mentioning the term Eastern Blotting. —Preceding
unsigned comment added by
70.241.90.85 (
talk)
04:32, 15 June 2009 (UTC)
Though Kornberg described the DNA polymerase, he did not find any practical applications for it. The name PCR (Polymerase Chain Reaction) (the practical technique of amplifying DNA) was described by Kary Mullis. Hence Kary Mullis is considered the inventor of PCR, not Kornberg who described the DNA polymerase the key ingredient of PCR. Similarly, lectins, Cholera Toxin B etc., were described by various scientists. None of them coined or used the term "Eastern Blotting" until S. Thomas described a practical way of detecting post translational protein modifications. —Preceding unsigned comment added by 129.109.98.97 ( talk) 14:39, 15 June 2009 (UTC)
From Wikipedia: Mullis was not the first to propose the ideas behind PCR. The main principles were described in 1971 by 1968 Nobel Prize laureate H. Gobind Khorana and Kjell Kleppe, a Norwegian scientist. Kleppe and Khorana released a 20-page research paper on PCR in the 1971 Journal of Molecular Biology. As early as June 18, 1969, Kleppe had presented his work at the Gordon Conference in New Hampshire. Using repair replication (the principle of PCR), he duplicated and then quadrupled a small synthetic molecule with the help of two primers and DNA-polymerase. However, "the full potential of PCR was not realized" until Mullis' work in 1983.
Eastern Blotting was not named until the paper of S. Thomas describing detection of post translational protein modifications. It is just not lectin blotting alone. There are 25 post-translational protein modifications and over the years various substrates have been used to detect different moities (including phosphorylation, lipoylation etc) with different substrates. None of them coined the term "Eastern Blotting". Techniques detecting post translational modifications is Eastern Blotting. The article is written for "Eastern Blotting", not any other blotting techniques. —Preceding unsigned comment added by 129.109.98.97 ( talk) 21:38, 16 June 2009 (UTC)
This article is about Eastern Blotting. Only references mentioning the technique of "Eastern Blotting" (detection of post translational protein modification) are to be included in this article. —Preceding unsigned comment added by 129.109.98.107 ( talk) 14:50, 17 June 2009 (UTC)
The lectin blotting reference mentioned by Glycoform: Tanner and Anstee (1976) is not a blot study. The paper never mentions blot. The assay used by Tanner and Anstee is "gel radiolabelled lectin assay". The authors never even showed the radiolabelled gel (which I believe could be shown on an X-ray film) on that paper, but marked the distance calculated as a "graph" (which no scientific paper now consider as legitimate). The first blotting technique Southern Blotting was described in 1975 and Western blotting in 1979!!! —Preceding unsigned comment added by 129.109.98.97 ( talk) 15:22, 17 June 2009 (UTC)
I have taken the liberty of restoring the version that gives ample and even credit to both the naming convention, as well as the pivotal back-work for the application.
Wisdom89 (
T /
C)
16:13, 17 June 2009 (UTC)
Southern Blotting was published in Nov. 1975 (the first blotting technique). Tanner and Anstee send their paper on lectin to Biochemical Journal in 1975 (before the Southern Blotting was published)!!!! Tanner and Anstee never used a blot!!!!! It makes no sense why their name and paper is included as a reference in Eastern Blotting. —Preceding unsigned comment added by 129.109.98.97 ( talk) 16:48, 17 June 2009 (UTC)
What is Far-Eastern blot and In gel lectin assay doing in Eastern Blotting Section? Not good Editors!!!!! —Preceding unsigned comment added by 70.241.98.153 ( talk) 04:03, 18 June 2009 (UTC)
Who coined the term Eastern Blotting (the detection of post translational protein modification)? —Preceding unsigned comment added by 70.241.111.107 ( talk) 20:15, 18 June 2009 (UTC)
I presume the Editors do not read papers carefully: (The term "Eastern blotting" is used only rarely in primary literature, and has appeared more in recent publications (Ishikawa D and Taki T,1998). Even Ishikawa and Taki will be laughing at this wrong quote. Ishikawa and Taki developed the Far-Eastern Blotting (not Eastern Blotting). By the way, if you people do not know what is Far-Eastern Blotting, kindly take some time to read it. —Preceding unsigned comment added by 129.109.98.97 ( talk) 23:48, 18 June 2009 (UTC)
“The technique of Far-Eastern blotting was developed by Ishikawa and Taki (1998) as a method for transferring lipids from an HPTLC plate to a PVDF membrane and later probed with antibodies. The technique was also used by Fukuda et al. (2006) to separate ginsenosides by TLC and blotted to a PVDF membrane treated with NaIO4 solution followed by bovine serum albumin (BSA) which resulted in a ginsenoside–BSA conjugate on the PVDF membrane. The blotted spots were finally stained by antiginsenoside monoclonal antibodies. Though the technique used is the same Far-Eastern technique described by Ishikawa and Taki (1998) the authors used the term Eastern blot for their studies!!!” (Text quoted from- Thomas et al., 2009)
A summary of more recent improvements of the protocol has been provided by H. Freeze (1993)- It is 16 years- still you call it RECENT. —Preceding unsigned comment added by 129.109.98.97 ( talk) 23:35, 29 June 2009 (UTC)
{{
editprotected}}
Administrators:
this version of the page appears to be the version generally accepted by established editors at this time. Recognizing that this may change as the discussion progresses, please revert this article to the preferred version. Editors: if I picked the wrong revision, please correct me.
Zab
Milenko
How am I driving?
15:11, 15 June 2009 (UTC)
As Rootology has announced a wikibreak, and per the discussion above, I have reduced the protection to semi. To the unregistered user: please discuss your changes on this page first and then add {{ editsemiprotected}} if a consensus develops. Regards, — Martin ( MSGJ · talk) 22:15, 15 June 2009 (UTC)
Based on the controversy here, I did some digging in the literature. I was able to find no fewer than five papers that claim to have named the Eastern blot. Many of these are quite similar, and not just new applications of a technique that they use the term for. I tried to include only papers that had specific text stating that this was a new technique (some even put it in the title of the paper.) I think the article needs to acknowledge all of these, and should probably reflect the lack of consensus in the primary literature. It may also be worth re-assessing which articles to highlight as applications. To be fair, all versions of the term should have some examples, but this makes for a large addition of text.Any other examples welcome, especially any that predate those included already.-- Glycoform ( talk) 22:35, 27 June 2009 (UTC)
I have to take issue with this edit; the paragraph in question is heavily referenced, and contains several indisputable facts. For example, the interview and comment by Ed Southern (and paper which was commented on), as well as the appearance of the term in textbooks. Both are entirely relevant to this article. If you have issue with the limited context provided (i.e. pointing out that the referenced article is inconsistent with a second referenced article), I could understand editing it. (Although these two authors are in clear conflict with each other - this is not opinion). As I see it, this edit deletes relevant, and referenced, information from the article. I don't agree that its "slanted" in any way, but if you care to revise to unslant it, please do so without losing other relevant content.-- Glycoform ( talk) 00:32, 16 July 2010 (UTC)
An Eastern Blot is when you run a Western with the polarity backward so all your protein runs off the gel immediately. At least 5 people I know use the term this way, although most of us only know of this Eastern as legend and not from personal experience ;). -- Chinasaur ( talk) 01:15, 3 March 2011 (UTC)
Hello fellow Wikipedians,
I have just added archive links to one external link on
Eastern blot. Please take a moment to review
my edit. If necessary, add {{
cbignore}}
after the link to keep me from modifying it. Alternatively, you can add {{
nobots|deny=InternetArchiveBot}}
to keep me off the page altogether. I made the following changes:
When you have finished reviewing my changes, please set the checked parameter below to true to let others know.
This message was posted before February 2018.
After February 2018, "External links modified" talk page sections are no longer generated or monitored by InternetArchiveBot. No special action is required regarding these talk page notices, other than
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RfC before doing mass systematic removals. This message is updated dynamically through the template {{
source check}}
(last update: 5 June 2024).
Cheers.— cyberbot II Talk to my owner:Online 14:33, 24 February 2016 (UTC)