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This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available
on the course page. Student editor(s):
Nathalieli. Peer reviewers:
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Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT ( talk) 19:45, 17 January 2022 (UTC)
This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available
on the course page. Student editor(s):
Axiao12,
Joyspark,
Ankita5195. Peer reviewers:
Joyspark,
Sbudhoo11,
Ankita5195.
Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT ( talk) 18:57, 16 January 2022 (UTC)
A proposal has been made to merge sticky end/blunt end here. My personal reaction is, wouldn't it be more logical to merge into restriction enzyme? // habj 13:27, 8 October 2006 (UTC)
Ntokozo Masina —Preceding unsigned comment added by 196.21.218.142 ( talk) 17:17, 14 May 2008 (UTC)
DNA ligase I: connects Okazaki fragments of the lagging strand in DNA replication, and can also seal some repair and recombination fragments (Wei et al., 1995).
so it can ligate single strand breaks, article is pretty unprecise
This article says that ATP is not required for a ligase reaction. This is wrong. ATP is required, no energy is derived from the DNA backbone because the 5' phosphate is a mono phosphate nor is it cleaved. I am changing the article. Adenosine talk 08:24, 17 October 2008 (UTC)
There are some confusions in the bactieral DNA ligase section of this article. I'll try and sort it out when I get the time. The key bacterial ligases require NAD+. Some also have ATP-dependent ligases, but it's bacteriopages that sometimes have smaller, ATP-dependent ligases. England Expects ( talk) 12:49, 31 March 2009 (UTC)
The author talks about the necessity of controlling temperature in molecular biology reactions in order to balance the optimal activity of T4 ligase with the Tm of the fragments being ligated. I'm expanding and updating this to include sources. Ksalerno ( talk) 17:31, 18 August 2009 (UTC) Kat Salerno
Does the second part of the first sentence contradict the first part of it? It seems to say that ligase repairs single-stranded breaks, then goes on to note that they are actually double-stranded breaks. — Preceding unsigned comment added by 155.52.37.140 ( talk) 15:01, 19 July 2011 (UTC)
The article says that Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template. In what sense is the complementary strand used as a template? Polymerase does use the complementary strand as a template when repairing some forms of DNA damage, and that is followed by ligation. Perhaps that is what is meant; as it stands, I believe the article is incorrect. AlphaHelical ( talk) 04:05, 30 May 2013 (UTC)
![]() | This ![]() It is of interest to the following WikiProjects: | |||||||||||||
|
This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available
on the course page. Student editor(s):
Nathalieli. Peer reviewers:
Nathalieli.
Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT ( talk) 19:45, 17 January 2022 (UTC)
This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available
on the course page. Student editor(s):
Axiao12,
Joyspark,
Ankita5195. Peer reviewers:
Joyspark,
Sbudhoo11,
Ankita5195.
Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT ( talk) 18:57, 16 January 2022 (UTC)
A proposal has been made to merge sticky end/blunt end here. My personal reaction is, wouldn't it be more logical to merge into restriction enzyme? // habj 13:27, 8 October 2006 (UTC)
Ntokozo Masina —Preceding unsigned comment added by 196.21.218.142 ( talk) 17:17, 14 May 2008 (UTC)
DNA ligase I: connects Okazaki fragments of the lagging strand in DNA replication, and can also seal some repair and recombination fragments (Wei et al., 1995).
so it can ligate single strand breaks, article is pretty unprecise
This article says that ATP is not required for a ligase reaction. This is wrong. ATP is required, no energy is derived from the DNA backbone because the 5' phosphate is a mono phosphate nor is it cleaved. I am changing the article. Adenosine talk 08:24, 17 October 2008 (UTC)
There are some confusions in the bactieral DNA ligase section of this article. I'll try and sort it out when I get the time. The key bacterial ligases require NAD+. Some also have ATP-dependent ligases, but it's bacteriopages that sometimes have smaller, ATP-dependent ligases. England Expects ( talk) 12:49, 31 March 2009 (UTC)
The author talks about the necessity of controlling temperature in molecular biology reactions in order to balance the optimal activity of T4 ligase with the Tm of the fragments being ligated. I'm expanding and updating this to include sources. Ksalerno ( talk) 17:31, 18 August 2009 (UTC) Kat Salerno
Does the second part of the first sentence contradict the first part of it? It seems to say that ligase repairs single-stranded breaks, then goes on to note that they are actually double-stranded breaks. — Preceding unsigned comment added by 155.52.37.140 ( talk) 15:01, 19 July 2011 (UTC)
The article says that Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template. In what sense is the complementary strand used as a template? Polymerase does use the complementary strand as a template when repairing some forms of DNA damage, and that is followed by ligation. Perhaps that is what is meant; as it stands, I believe the article is incorrect. AlphaHelical ( talk) 04:05, 30 May 2013 (UTC)