| SabinâFeldman dye test | |
|---|---|
| Purpose | diagnose for toxoplasmosis. |
A SabinâFeldman dye test is a serologic test to diagnose for toxoplasmosis. Patient serum is treated with Toxoplasma trophozoites and complement, and then incubated. After incubation, methylene blue is added. If anti-Toxo antibodies are present in the serum, the antibody-antigen complex activates complement to lyse the parasite membrane, Toxoplasma trophozoites are not stained (positive result); if there are no antibodies, trophozoites with intact membrane are stained and appear blue under microscope (negative result). The dilution of the test serum at which 50% of the tachyzoites are thin, distorted and colorless is reported as antibody titer of the test serum. The test is highly sensitive and specific with no false positives reported so far.[ clarification needed]
Drawbacks of this test: 1. Difficulty in maintaining the live tachyzoites. 2. It detects immunoglobulin G(IgG) antibodies, hence cannot differentiate between recent or past infection. 3.False positive for Sarcocystis, Trypanosoma lewisi, Trichomonas vaginalis[ clarification needed]
References
- Mosby's Medical, Nursing, & Allied Health Dictionary. Edition 5, 1998 p7B4A.
 | This medical diagnostic article is a stub. You can help Wikipedia by expanding it. |
| SabinâFeldman dye test | |
|---|---|
| Purpose | diagnose for toxoplasmosis. |
A SabinâFeldman dye test is a serologic test to diagnose for toxoplasmosis. Patient serum is treated with Toxoplasma trophozoites and complement, and then incubated. After incubation, methylene blue is added. If anti-Toxo antibodies are present in the serum, the antibody-antigen complex activates complement to lyse the parasite membrane, Toxoplasma trophozoites are not stained (positive result); if there are no antibodies, trophozoites with intact membrane are stained and appear blue under microscope (negative result). The dilution of the test serum at which 50% of the tachyzoites are thin, distorted and colorless is reported as antibody titer of the test serum. The test is highly sensitive and specific with no false positives reported so far.[ clarification needed]
Drawbacks of this test: 1. Difficulty in maintaining the live tachyzoites. 2. It detects immunoglobulin G(IgG) antibodies, hence cannot differentiate between recent or past infection. 3.False positive for Sarcocystis, Trypanosoma lewisi, Trichomonas vaginalis[ clarification needed]
References
- Mosby's Medical, Nursing, & Allied Health Dictionary. Edition 5, 1998 p7B4A.
|
| This medical diagnostic article is a stub. You can help Wikipedia by expanding it. |