Src kinase-associated phosphoprotein 1 is an
adapter protein that in humans is encoded by the SKAP1gene.[5][6]
This gene encodes a
T cell adapter protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a
PH domain and C-terminal
SH3 domain. Along with the adhesion and degranulation-promoting adapter protein, the encoded protein plays a critical role in
inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of
integrins.[6]
The demonstration that SKAP1 regulates
LFA-1 adhesion was made by retroviral infection [7] and by the SKAP1 deficient mouse.[8] Additional work has implicated SKAP1 in binding to the
exchange factorRasGRP1 and in regulating
ERK activation in T-cells.[9][10]
^Wang H, Moon EY, Azouz A, Wu X, Smith A, Schneider H, Hogg N, Rudd CE (April 2003). "SKAP-55 regulates integrin adhesion and formation of T cell-APC conjugates". Nature Immunology. 4 (4): 366–74.
doi:
10.1038/ni913.
PMID12652296.
S2CID11810379.
^Wang H, Liu H, Lu Y, Lovatt M, Wei, B, Rudd CE Functional defects of SKAP-55 deficient T-cells identify a regulatory role for the adaptor in LFA-1 adhesion, Mol. Cell. Biol. 2007 27, 6863-75.
^* Kosco KA, Cerignoli F, Williams S, Abraham RT, Mustelin T (January 2008). "SKAP55 modulates T cell antigen receptor-induced activation of the Ras-Erk-AP1 pathway by binding RasGRP1". Molecular Immunology. 45 (2): 510–22.
doi:
10.1016/j.molimm.2007.05.024.
PMID17658605.
^Schneider H., Wang H., Raab M., Valk E., Smith X. , Lovatt M., Wu Z., Strebhardt K, Rudd, CE. Adaptor SKAP55 binds RasGRP1 and negatively regulates p21ras and the ERK pathway in T-cells. (2008) PLoS ONE. 3, 1718.
Schraven B, Marie-Cardine A, Koretzky G (June 1997). "Molecular analysis of the fyn-complex: cloning of SKAP55 and SLAP-130, two novel adaptor proteins which associate with fyn and may participate in the regulation of T cell receptor-mediated signaling". Immunology Letters. 57 (1–3): 165–9.
doi:
10.1016/S0165-2478(97)00053-9.
PMID9232446.
Clements JL, Boerth NJ, Lee JR, Koretzky GA (1999). "Integration of T cell receptor-dependent signaling pathways by adapter proteins". Annual Review of Immunology. 17: 89–108.
doi:
10.1146/annurev.immunol.17.1.89.
PMID10358754.
Kouroku Y, Soyama A, Fujita E, Urase K, Tsukahara T, Momoi T (November 1998). "RA70 is a src kinase-associated protein expressed ubiquitously". Biochemical and Biophysical Research Communications. 252 (3): 738–42.
doi:
10.1006/bbrc.1998.9637.
PMID9837776.
Src kinase-associated phosphoprotein 1 is an
adapter protein that in humans is encoded by the SKAP1gene.[5][6]
This gene encodes a
T cell adapter protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a
PH domain and C-terminal
SH3 domain. Along with the adhesion and degranulation-promoting adapter protein, the encoded protein plays a critical role in
inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of
integrins.[6]
The demonstration that SKAP1 regulates
LFA-1 adhesion was made by retroviral infection [7] and by the SKAP1 deficient mouse.[8] Additional work has implicated SKAP1 in binding to the
exchange factorRasGRP1 and in regulating
ERK activation in T-cells.[9][10]
^Wang H, Moon EY, Azouz A, Wu X, Smith A, Schneider H, Hogg N, Rudd CE (April 2003). "SKAP-55 regulates integrin adhesion and formation of T cell-APC conjugates". Nature Immunology. 4 (4): 366–74.
doi:
10.1038/ni913.
PMID12652296.
S2CID11810379.
^Wang H, Liu H, Lu Y, Lovatt M, Wei, B, Rudd CE Functional defects of SKAP-55 deficient T-cells identify a regulatory role for the adaptor in LFA-1 adhesion, Mol. Cell. Biol. 2007 27, 6863-75.
^* Kosco KA, Cerignoli F, Williams S, Abraham RT, Mustelin T (January 2008). "SKAP55 modulates T cell antigen receptor-induced activation of the Ras-Erk-AP1 pathway by binding RasGRP1". Molecular Immunology. 45 (2): 510–22.
doi:
10.1016/j.molimm.2007.05.024.
PMID17658605.
^Schneider H., Wang H., Raab M., Valk E., Smith X. , Lovatt M., Wu Z., Strebhardt K, Rudd, CE. Adaptor SKAP55 binds RasGRP1 and negatively regulates p21ras and the ERK pathway in T-cells. (2008) PLoS ONE. 3, 1718.
Schraven B, Marie-Cardine A, Koretzky G (June 1997). "Molecular analysis of the fyn-complex: cloning of SKAP55 and SLAP-130, two novel adaptor proteins which associate with fyn and may participate in the regulation of T cell receptor-mediated signaling". Immunology Letters. 57 (1–3): 165–9.
doi:
10.1016/S0165-2478(97)00053-9.
PMID9232446.
Clements JL, Boerth NJ, Lee JR, Koretzky GA (1999). "Integration of T cell receptor-dependent signaling pathways by adapter proteins". Annual Review of Immunology. 17: 89–108.
doi:
10.1146/annurev.immunol.17.1.89.
PMID10358754.
Kouroku Y, Soyama A, Fujita E, Urase K, Tsukahara T, Momoi T (November 1998). "RA70 is a src kinase-associated protein expressed ubiquitously". Biochemical and Biophysical Research Communications. 252 (3): 738–42.
doi:
10.1006/bbrc.1998.9637.
PMID9837776.