Ruberedoxin A (RubA) is a protein conserved across all studied oxygenic photoautotrophs. [1]
As of March 2015 there was no crystal structure of RubA although a structure of the homologous protein from a cryptomonad was determined using NMR. [2] Investigation of the gene however indicates that it differs from other known rubredoxins in being bound to the thylakoid membrane via a C-terminal transmembrane helix. [1] [3] [4]
An investigation of Guillardia theta noted that RubA had a similar distribution to Photosystem II (PSII) and immunological experiments indicated the presence of RubA in PSII complexes isolated from Spinacia oleracea. [3] In Synechocystis sp. PCC 6803 it has been demonstrated that the insertion of an antibiotic cassette into the rubA gene results in a marked decrease in the amount of PSII present, [1] while the same mutation within Chlamydomonas reinhardtii and in Arabidopsis thaliana results in a total absence of PSII. [1]
Conversely, another study performed in Synechococcus sp. PCC 7002 indicated that mutation of the rubA gene interfered with iron-sulphur cluster assembly in Photosystem I (PSI), rather than affecting PSII. [4] [5]
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: CS1 maint: multiple names: authors list (
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{{
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: CS1 maint: multiple names: authors list (
link)
Ruberedoxin A (RubA) is a protein conserved across all studied oxygenic photoautotrophs. [1]
As of March 2015 there was no crystal structure of RubA although a structure of the homologous protein from a cryptomonad was determined using NMR. [2] Investigation of the gene however indicates that it differs from other known rubredoxins in being bound to the thylakoid membrane via a C-terminal transmembrane helix. [1] [3] [4]
An investigation of Guillardia theta noted that RubA had a similar distribution to Photosystem II (PSII) and immunological experiments indicated the presence of RubA in PSII complexes isolated from Spinacia oleracea. [3] In Synechocystis sp. PCC 6803 it has been demonstrated that the insertion of an antibiotic cassette into the rubA gene results in a marked decrease in the amount of PSII present, [1] while the same mutation within Chlamydomonas reinhardtii and in Arabidopsis thaliana results in a total absence of PSII. [1]
Conversely, another study performed in Synechococcus sp. PCC 7002 indicated that mutation of the rubA gene interfered with iron-sulphur cluster assembly in Photosystem I (PSI), rather than affecting PSII. [4] [5]
{{
cite journal}}
: CS1 maint: multiple names: authors list (
link)
{{
cite journal}}
: CS1 maint: multiple names: authors list (
link)
{{
cite journal}}
: CS1 maint: multiple names: authors list (
link)
{{
cite journal}}
: CS1 maint: multiple names: authors list (
link)
{{
cite journal}}
: CS1 maint: multiple names: authors list (
link)