RprA RNA | |
---|---|
![]() Predicted
secondary structure and
sequence conservation of RprA | |
Identifiers | |
Symbol | RprA |
Rfam | RF00034 |
Other data | |
RNA type | Gene; sRNA |
Domain(s) | Bacteria |
SO | SO:0000387 |
PDB structures | PDBe |
The RprA RNA gene encodes a 106 nucleotide regulatory non-coding RNA. Translational regulation of the stationary phase sigma factor RpoS is mediated by the formation of a double-stranded RNA stem-loop structure in the upstream region of the rpoS messenger RNA, occluding the translation initiation site. [1] [2]
Clones carrying rprA (RpoS regulator RNA A) increased the translation of RpoS. As with DsrA, RprA is predicted to form three stem-loops. Thus, at least two small RNAs, DsrA and RprA, participate in the positive regulation of RpoS translation. RprA also appears to bind to the RpoS leader. [3] RprA is non-essential. [4] Wasserman et al. demonstrated that this RNA is bound by the Hfq protein. [5] Binding to Hfq alters the conformation of RprA. [6] In the presence of Hfq the stability of RprA is influenced by the osmolarity of the cell, this is dependent on the endoribonuclease RNase E. [7]
It has been shown the RprA regulates the protein coding genes, called csgD, this protein encodes a stationary phase-induced biofilm regulator and ydaM, which encodes a diguanylate cyclase involved in activating csgD transcription. These two target genes are repressed by RprA which results in regulation of biofilm formation. [8]
RprA RNA | |
---|---|
![]() Predicted
secondary structure and
sequence conservation of RprA | |
Identifiers | |
Symbol | RprA |
Rfam | RF00034 |
Other data | |
RNA type | Gene; sRNA |
Domain(s) | Bacteria |
SO | SO:0000387 |
PDB structures | PDBe |
The RprA RNA gene encodes a 106 nucleotide regulatory non-coding RNA. Translational regulation of the stationary phase sigma factor RpoS is mediated by the formation of a double-stranded RNA stem-loop structure in the upstream region of the rpoS messenger RNA, occluding the translation initiation site. [1] [2]
Clones carrying rprA (RpoS regulator RNA A) increased the translation of RpoS. As with DsrA, RprA is predicted to form three stem-loops. Thus, at least two small RNAs, DsrA and RprA, participate in the positive regulation of RpoS translation. RprA also appears to bind to the RpoS leader. [3] RprA is non-essential. [4] Wasserman et al. demonstrated that this RNA is bound by the Hfq protein. [5] Binding to Hfq alters the conformation of RprA. [6] In the presence of Hfq the stability of RprA is influenced by the osmolarity of the cell, this is dependent on the endoribonuclease RNase E. [7]
It has been shown the RprA regulates the protein coding genes, called csgD, this protein encodes a stationary phase-induced biofilm regulator and ydaM, which encodes a diguanylate cyclase involved in activating csgD transcription. These two target genes are repressed by RprA which results in regulation of biofilm formation. [8]