RNA polymerase sigma-54 factor | |||||||
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Identifiers | |||||||
Organism | ? | ||||||
Symbol | RpoN | ||||||
UniProt | P24255 | ||||||
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The gene rpoN (RNA polymerase, nitrogen-limitation N) encodes the sigma factor sigma-54 (σ54, sigma N, or RpoN), a protein in Escherichia coli and other species of bacteria. RpoN antagonizes RpoS sigma factors. [1]
Originally identified as a regulator of genes involved in nitrogen metabolism and assimilation under nitrogen limiting conditions, [2] E. coli σ54 has since been shown to play important regulatory roles in a variety of other cellular processes. Similarly, σ54 homologues in other species regulate a wide range of processes, including flagellar synthesis and virulence. [3]
Sigma-54 factor, Activator interacting domain | |||||||||||
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Identifiers | |||||||||||
Symbol | Sigma54_AID | ||||||||||
Pfam | PF00309 | ||||||||||
InterPro | IPR000394 | ||||||||||
PROSITE | PS50044 | ||||||||||
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σ54 promoter elements consist of conserved nucleotides located at −12 and −24 with respect to the transcription start site. [4] This contrasts with members of the σ70 family, which recognize conserved promoter elements located at roughly −10 and −35 with respect to the transcription start site. [5] Unlike the members of the σ70 family, σ54 proteins have been shown to bind promoter DNA independent of core RNAPin vitro. [6] Another distinguishing characteristic of σ54 proteins is their absolute requirement for activator proteins, known as bacterial enhancer binding proteins (bEBPs), to initiate transcription. [4] [7] Thus, both active and inactive forms of RNAP:σ54 are bound at promoters. [8]
The RpoN-regulated promoter elements have the consensus sequence as follows: TTGGCACGGTTTTTGCT. [9]
RNA polymerase sigma-54 factor | |||||||
---|---|---|---|---|---|---|---|
Identifiers | |||||||
Organism | ? | ||||||
Symbol | RpoN | ||||||
UniProt | P24255 | ||||||
|
The gene rpoN (RNA polymerase, nitrogen-limitation N) encodes the sigma factor sigma-54 (σ54, sigma N, or RpoN), a protein in Escherichia coli and other species of bacteria. RpoN antagonizes RpoS sigma factors. [1]
Originally identified as a regulator of genes involved in nitrogen metabolism and assimilation under nitrogen limiting conditions, [2] E. coli σ54 has since been shown to play important regulatory roles in a variety of other cellular processes. Similarly, σ54 homologues in other species regulate a wide range of processes, including flagellar synthesis and virulence. [3]
Sigma-54 factor, Activator interacting domain | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Identifiers | |||||||||||
Symbol | Sigma54_AID | ||||||||||
Pfam | PF00309 | ||||||||||
InterPro | IPR000394 | ||||||||||
PROSITE | PS50044 | ||||||||||
|
σ54 promoter elements consist of conserved nucleotides located at −12 and −24 with respect to the transcription start site. [4] This contrasts with members of the σ70 family, which recognize conserved promoter elements located at roughly −10 and −35 with respect to the transcription start site. [5] Unlike the members of the σ70 family, σ54 proteins have been shown to bind promoter DNA independent of core RNAPin vitro. [6] Another distinguishing characteristic of σ54 proteins is their absolute requirement for activator proteins, known as bacterial enhancer binding proteins (bEBPs), to initiate transcription. [4] [7] Thus, both active and inactive forms of RNAP:σ54 are bound at promoters. [8]
The RpoN-regulated promoter elements have the consensus sequence as follows: TTGGCACGGTTTTTGCT. [9]