From Wikipedia, the free encyclopedia
Peptide sequence in short-lived proteins
A PEST sequence is a
peptide sequence that is rich in
proline (P),
glutamic acid (E),
serine (S) and
threonine (T). It is associated with
proteins that have a short intracellular
half-life, so might act as a
signal peptide for
protein degradation.
[1] This may be mediated via the
proteasome
[2]
[3] or
calpain.
[4]
-
^ Rogers S, Wells R, Rechsteiner M (1986). "Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis".
Science. 234 (4774): 364–8.
Bibcode:
1986Sci...234..364R.
doi:
10.1126/science.2876518.
PMID
2876518.
-
^ Reverte CG, Ahearn MD, Hake LE (2001).
"CPEB degradation during Xenopus oocyte maturation requires a PEST domain and the 26S proteasome". Dev. Biol. 231 (2): 447–58.
doi:
10.1006/dbio.2001.0153.
PMID
11237472.
-
^ Spencer ML, Theodosiou M, Noonan DJ (2004).
"NPDC-1, a novel regulator of neuronal proliferation, is degraded by the ubiquitin/proteasome system through a PEST degradation motif". J. Biol. Chem. 279 (35): 37069–78.
doi:
10.1074/jbc.M402507200.
PMID
15229225.
-
^ Shumway SD, Maki M, Miyamoto S (1999).
"The PEST Domain of IκBα is necessary and sufficient for in vitro degradation by mu-calpain". J. Biol. Chem. 274 (43): 30874–81.
doi:
10.1074/jbc.274.43.30874.
PMID
10521480.