Myoblast city | |||||||
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Identifiers | |||||||
Organism | |||||||
Symbol | mbc | ||||||
UniProt | Q9VCH4 | ||||||
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Myoblast city (Mbc [1]) is the Drosophila melanogaster ortholog of the mammalian protein Dock180. [2] Mutant mbc embryos exhibit defects in dorsal closure, cytoskeletal organization, myogenesis, and neural development. [2] [3]
The Myoblast city locus was identified by deletion mapping, using this technique researchers were able to isolate the location of the gene on the right arm of the third chromosome. [4] During the process four recessive alleles of Mbc were found; mbcc1, mbcc2, mbcc3, mbcs4, all of which are lethal and the D. melanogaster embryos fail to hatch. [4]
During the first 9-10 hours of development, embryos with the mutant Mbc alleles show the same myosin expression as wild-type embryos. [4] However, at about the 11th hour, most myoblasts fail to fuse. As development progresses, some myoblasts show signs of fusion, such as elongation and having multiple nuclei; but some remain round. [4] After 13-14 hours of development, most cells in mutant embryos that failed to fuse lose myosin expression. [4]
Myoblast city | |||||||
---|---|---|---|---|---|---|---|
Identifiers | |||||||
Organism | |||||||
Symbol | mbc | ||||||
UniProt | Q9VCH4 | ||||||
|
Myoblast city (Mbc [1]) is the Drosophila melanogaster ortholog of the mammalian protein Dock180. [2] Mutant mbc embryos exhibit defects in dorsal closure, cytoskeletal organization, myogenesis, and neural development. [2] [3]
The Myoblast city locus was identified by deletion mapping, using this technique researchers were able to isolate the location of the gene on the right arm of the third chromosome. [4] During the process four recessive alleles of Mbc were found; mbcc1, mbcc2, mbcc3, mbcs4, all of which are lethal and the D. melanogaster embryos fail to hatch. [4]
During the first 9-10 hours of development, embryos with the mutant Mbc alleles show the same myosin expression as wild-type embryos. [4] However, at about the 11th hour, most myoblasts fail to fuse. As development progresses, some myoblasts show signs of fusion, such as elongation and having multiple nuclei; but some remain round. [4] After 13-14 hours of development, most cells in mutant embryos that failed to fuse lose myosin expression. [4]