Monopolin is a
protein complex that in budding yeast is composed of the four proteins
CSM1,
HRR25,
LRS4, and
MAM1. Monopolin is required for the segregation of homologous
centromeres to opposite poles of a dividing cell during
anaphase I of meiosis.[1] This occurs by bridging
DSN1 kinetochore proteins to
sister kinetochores within the centromere to physically fuse them and allow for the microtubules to pull each homolog toward opposite
mitotic spindles.[2]
Molecular structure
Monopolin is composed of a 4 CSM1:2 LRS4 complex which forms a V-shaped structure with two globular heads at the ends, which are responsible for directly crosslinking sister kinetochores.[1] Bound to each CSM1 head is a MAM1 protein which recruits one copy of the HRR25 kinase.[3] The hydrophobic cavity on the CSM1 subunit allows the hydrophobic regions of Monopolin receptor and kinetochore protein,
DSN1, to bind to and fuse the sister kinetochores.[2]Microtubules can then attach to the kinetochores on the homologous centromeres and pull them toward opposite mitotic spindles to complete anaphase of meiosis I.
Monopolin is a
protein complex that in budding yeast is composed of the four proteins
CSM1,
HRR25,
LRS4, and
MAM1. Monopolin is required for the segregation of homologous
centromeres to opposite poles of a dividing cell during
anaphase I of meiosis.[1] This occurs by bridging
DSN1 kinetochore proteins to
sister kinetochores within the centromere to physically fuse them and allow for the microtubules to pull each homolog toward opposite
mitotic spindles.[2]
Molecular structure
Monopolin is composed of a 4 CSM1:2 LRS4 complex which forms a V-shaped structure with two globular heads at the ends, which are responsible for directly crosslinking sister kinetochores.[1] Bound to each CSM1 head is a MAM1 protein which recruits one copy of the HRR25 kinase.[3] The hydrophobic cavity on the CSM1 subunit allows the hydrophobic regions of Monopolin receptor and kinetochore protein,
DSN1, to bind to and fuse the sister kinetochores.[2]Microtubules can then attach to the kinetochores on the homologous centromeres and pull them toward opposite mitotic spindles to complete anaphase of meiosis I.