Host cell factor 1 (HCFC1, HCF1, or HCF-1), also known as VP16-accessory protein,[5] is a
protein that in humans is encoded by the HCFC1gene.[6][7]
Structure
HCF1 is a member of the highly conserved host cell factor family and encodes a protein with five
Kelch repeats, a
fibronectin-like motif, and six
HCF repeats, each of which contains a highly specific cleavage signal. This nuclear
transcription coregulator is
proteolytically cleaved at one or more of the six possible sites, resulting in the creation of an
N-terminal chain and the corresponding
C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains which interact through electrostatic forces.
Function
HCF1 is involved in control of the
cell cycle as well as having regulatory roles in a multitude of processes related to
transcription. Additionally, work in model organisms point to HCF1 as being a putative
longevity determinant.[8] Alternatively
spliced variants that encode different protein isoforms have been described; however, not all variants have been fully characterized.[7]
Mutations in this gene have been linked to disorders of the
cobalamine metabolism.[9]
Interactions
Host cell factor C1 has been shown to
interact with:
^Wilson AC, LaMarco K, Peterson MG, Herr W (Jul 1993). "The VP16 accessory protein HCF is a family of polypeptides processed from a large precursor protein". Cell. 74 (1): 115–25.
doi:
10.1016/0092-8674(93)90299-6.
PMID8392914.
S2CID43569318.
^Gunther M, Laithier M, Brison O (Jul 2000). "A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening". Molecular and Cellular Biochemistry. 210 (1–2): 131–42.
doi:
10.1023/A:1007177623283.
PMID10976766.
S2CID1339642.
Frattini A, Faranda S, Redolfi E, Zucchi I, Villa A, Patrosso MC, Strina D, Susani L, Vezzoni P (Sep 1994). "Genomic organization of the human VP16 accessory protein, a housekeeping gene (HCFC1) mapping to Xq28". Genomics. 23 (1): 30–5.
doi:
10.1006/geno.1994.1455.
PMID7829097.
Zoppè M, Frattini A, Faranda S, Vezzoni P (May 1996). "The complete sequence of the host cell factor 1 (HCFC1) gene and its promoter: a role for YY1 transcription factor in the regulation of its expression". Genomics. 34 (1): 85–91.
doi:
10.1006/geno.1996.0244.
PMID8661027.
Host cell factor 1 (HCFC1, HCF1, or HCF-1), also known as VP16-accessory protein,[5] is a
protein that in humans is encoded by the HCFC1gene.[6][7]
Structure
HCF1 is a member of the highly conserved host cell factor family and encodes a protein with five
Kelch repeats, a
fibronectin-like motif, and six
HCF repeats, each of which contains a highly specific cleavage signal. This nuclear
transcription coregulator is
proteolytically cleaved at one or more of the six possible sites, resulting in the creation of an
N-terminal chain and the corresponding
C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains which interact through electrostatic forces.
Function
HCF1 is involved in control of the
cell cycle as well as having regulatory roles in a multitude of processes related to
transcription. Additionally, work in model organisms point to HCF1 as being a putative
longevity determinant.[8] Alternatively
spliced variants that encode different protein isoforms have been described; however, not all variants have been fully characterized.[7]
Mutations in this gene have been linked to disorders of the
cobalamine metabolism.[9]
Interactions
Host cell factor C1 has been shown to
interact with:
^Wilson AC, LaMarco K, Peterson MG, Herr W (Jul 1993). "The VP16 accessory protein HCF is a family of polypeptides processed from a large precursor protein". Cell. 74 (1): 115–25.
doi:
10.1016/0092-8674(93)90299-6.
PMID8392914.
S2CID43569318.
^Gunther M, Laithier M, Brison O (Jul 2000). "A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening". Molecular and Cellular Biochemistry. 210 (1–2): 131–42.
doi:
10.1023/A:1007177623283.
PMID10976766.
S2CID1339642.
Frattini A, Faranda S, Redolfi E, Zucchi I, Villa A, Patrosso MC, Strina D, Susani L, Vezzoni P (Sep 1994). "Genomic organization of the human VP16 accessory protein, a housekeeping gene (HCFC1) mapping to Xq28". Genomics. 23 (1): 30–5.
doi:
10.1006/geno.1994.1455.
PMID7829097.
Zoppè M, Frattini A, Faranda S, Vezzoni P (May 1996). "The complete sequence of the host cell factor 1 (HCFC1) gene and its promoter: a role for YY1 transcription factor in the regulation of its expression". Genomics. 34 (1): 85–91.
doi:
10.1006/geno.1996.0244.
PMID8661027.