Hypoxia up-regulated protein 1 is a
protein that in humans is encoded by the HYOU1gene.[5][6][7]
The protein encoded by this gene belongs to the
heat shock protein 70 family. This gene has three
mRNAs from the use of alternative transcription sites. A cis-acting segment is found at the 5' end of exon 1A which is involved in the stress-dependent induction. The transcript that begins with exon 1B is preferentially induced by
hypoxia, resulting in the accumulation of this protein in the
endoplasmic reticulum (ER).
The protein encoded by this gene is thought to play an important role in
protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated
apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in
breast tumors, and thus it is associated with tumor invasiveness.
There is also an alternative translation site of this gene which lacks the
signal peptide. This signal peptide-lacking protein, is only 3 amino acids shorter than the
mature protein in the ER, and it is thought to have a housekeeping function in the
cytosol.[7]
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4.
doi:
10.1016/0378-1119(94)90802-8.
PMID8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56.
doi:
10.1016/S0378-1119(97)00411-3.
PMID9373149.
Tsukamoto Y, Kuwabara K, Hirota S, et al. (1998). "Expression of the 150-kd oxygen-regulated protein in human breast cancer". Lab. Invest. 78 (6): 699–706.
PMID9645760.
Kaneda S, Yura T, Yanagi H (2000). "Production of three distinct mRNAs of 150 kDa oxygen-regulated protein (ORP150) by alternative promoters: preferential induction of one species under stress conditions". J. Biochem. 128 (3): 529–38.
doi:
10.1093/oxfordjournals.jbchem.a022783.
PMID10965054.
Tamatani M, Matsuyama T, Yamaguchi A, et al. (2001). "ORP150 protects against hypoxia/ischemia-induced neuronal death". Nat. Med. 7 (3): 317–23.
doi:
10.1038/85463.
PMID11231630.
S2CID10152200.
Zhang H, Li XJ, Martin DB, Aebersold R (2003). "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry". Nat. Biotechnol. 21 (6): 660–6.
doi:
10.1038/nbt827.
PMID12754519.
S2CID581283.
Ahmed M, Forsberg J, Bergsten P (2005). "Protein profiling of human pancreatic islets by two-dimensional gel electrophoresis and mass spectrometry". J. Proteome Res. 4 (3): 931–40.
doi:
10.1021/pr050024a.
PMID15952740.
Takeuchi S (2007). "Molecular cloning, sequence, function and structural basis of human heart 150 kDa oxygen-regulated protein, an ER chaperone". Protein J. 25 (7–8): 517–28.
doi:
10.1007/s10930-006-9038-z.
PMID17131193.
S2CID41452314.
Kitao Y, Matsuyama T, Takano K, et al. (2007). "Does ORP150/HSP12A protect dopaminergic neurons against MPTP/MPP(+)-induced neurotoxicity?". Antioxid. Redox Signal. 9 (5): 589–95.
doi:
10.1089/ars.2006.1518.
PMID17330988.
Hypoxia up-regulated protein 1 is a
protein that in humans is encoded by the HYOU1gene.[5][6][7]
The protein encoded by this gene belongs to the
heat shock protein 70 family. This gene has three
mRNAs from the use of alternative transcription sites. A cis-acting segment is found at the 5' end of exon 1A which is involved in the stress-dependent induction. The transcript that begins with exon 1B is preferentially induced by
hypoxia, resulting in the accumulation of this protein in the
endoplasmic reticulum (ER).
The protein encoded by this gene is thought to play an important role in
protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated
apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in
breast tumors, and thus it is associated with tumor invasiveness.
There is also an alternative translation site of this gene which lacks the
signal peptide. This signal peptide-lacking protein, is only 3 amino acids shorter than the
mature protein in the ER, and it is thought to have a housekeeping function in the
cytosol.[7]
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4.
doi:
10.1016/0378-1119(94)90802-8.
PMID8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56.
doi:
10.1016/S0378-1119(97)00411-3.
PMID9373149.
Tsukamoto Y, Kuwabara K, Hirota S, et al. (1998). "Expression of the 150-kd oxygen-regulated protein in human breast cancer". Lab. Invest. 78 (6): 699–706.
PMID9645760.
Kaneda S, Yura T, Yanagi H (2000). "Production of three distinct mRNAs of 150 kDa oxygen-regulated protein (ORP150) by alternative promoters: preferential induction of one species under stress conditions". J. Biochem. 128 (3): 529–38.
doi:
10.1093/oxfordjournals.jbchem.a022783.
PMID10965054.
Tamatani M, Matsuyama T, Yamaguchi A, et al. (2001). "ORP150 protects against hypoxia/ischemia-induced neuronal death". Nat. Med. 7 (3): 317–23.
doi:
10.1038/85463.
PMID11231630.
S2CID10152200.
Zhang H, Li XJ, Martin DB, Aebersold R (2003). "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry". Nat. Biotechnol. 21 (6): 660–6.
doi:
10.1038/nbt827.
PMID12754519.
S2CID581283.
Ahmed M, Forsberg J, Bergsten P (2005). "Protein profiling of human pancreatic islets by two-dimensional gel electrophoresis and mass spectrometry". J. Proteome Res. 4 (3): 931–40.
doi:
10.1021/pr050024a.
PMID15952740.
Takeuchi S (2007). "Molecular cloning, sequence, function and structural basis of human heart 150 kDa oxygen-regulated protein, an ER chaperone". Protein J. 25 (7–8): 517–28.
doi:
10.1007/s10930-006-9038-z.
PMID17131193.
S2CID41452314.
Kitao Y, Matsuyama T, Takano K, et al. (2007). "Does ORP150/HSP12A protect dopaminergic neurons against MPTP/MPP(+)-induced neurotoxicity?". Antioxid. Redox Signal. 9 (5): 589–95.
doi:
10.1089/ars.2006.1518.
PMID17330988.