Names | |
---|---|
Preferred IUPAC name
2,2′-{[2-(2-{2-[Bis(carboxymethyl)amino]-5-(2,7-difluoro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy}ethoxy)-4-methylphenyl]azanediyl}diacetic acid | |
Identifiers | |
3D model (
JSmol)
|
|
ChEBI | |
ChemSpider | |
MeSH | Fluo-4 |
PubChem
CID
|
|
UNII | |
CompTox Dashboard (
EPA)
|
|
| |
| |
Properties | |
C36H30F2N2O13 | |
Molar mass | 736.634 g·mol−1 |
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
|
Fluo-4 is used to measure calcium (Ca2+) concentrations inside living cells, and is often used for high-throughput screening of receptor ligands and calcium permeable ion channels.
The green-fluorescent calcium indicator, Fluo-4, is an improved version of the calcium indicator, Fluo-3. It is commonly used as the non-fluorescent acetoxymethyl ester (Fluo-4 AM) which is cleaved inside the cell to give the free, fluorescent Fluo-4. It loads faster, is brighter at equivalent concentrations and is well-excited by the 488 nm line of the argon-ion laser which is often used in biological research laboratories. [1] Fluo-4 and its cell-permeable AM ester are available from a few commercial vendors.
Names | |
---|---|
Preferred IUPAC name
2,2′-{[2-(2-{2-[Bis(carboxymethyl)amino]-5-(2,7-difluoro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy}ethoxy)-4-methylphenyl]azanediyl}diacetic acid | |
Identifiers | |
3D model (
JSmol)
|
|
ChEBI | |
ChemSpider | |
MeSH | Fluo-4 |
PubChem
CID
|
|
UNII | |
CompTox Dashboard (
EPA)
|
|
| |
| |
Properties | |
C36H30F2N2O13 | |
Molar mass | 736.634 g·mol−1 |
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
|
Fluo-4 is used to measure calcium (Ca2+) concentrations inside living cells, and is often used for high-throughput screening of receptor ligands and calcium permeable ion channels.
The green-fluorescent calcium indicator, Fluo-4, is an improved version of the calcium indicator, Fluo-3. It is commonly used as the non-fluorescent acetoxymethyl ester (Fluo-4 AM) which is cleaved inside the cell to give the free, fluorescent Fluo-4. It loads faster, is brighter at equivalent concentrations and is well-excited by the 488 nm line of the argon-ion laser which is often used in biological research laboratories. [1] Fluo-4 and its cell-permeable AM ester are available from a few commercial vendors.