DEP domain-containing mTOR-interacting protein (DEPTOR) also known as DEP domain-containing protein 6 (DEPDC6) is a
protein that in humans is encoded by the DEPTORgene.[5]
Structure
The gene DEPTOR can be found only in vertebrates. In human, DEPTOR gene locates at chromosome 8, 8q24.12 with protein size 409 a.a.[6] Human DEPTOR contains two
N-terminalDEP domains and a
C-terminalPDZ domain.[7]
Function
DEPTOR is involved in
mTOR signaling pathway as an endogenous regulator. A direct interaction between DEPTOR and mTOR has been shown. [7] Overexpression of DEPTOR downregulates the activity of
mTORC1 and
mTORC2in vitro. mTORC1 and mTORC2 can both inhibit DEPTOR through
phosphorylation.[7]
Metabolism
DEPTOR cell-autonomously regulates
adipogenesis.[8] In the muscle, Baf60c promotes a switch from oxidative to glycolytic myofiber type through DEPTOR-mediated
Akt/PKB activation.[9] Within the brain, DEPTOR is highly expressed in the
hippocampus, the medio-basal
hypothalamus and the
circumventricular organs.[10] Overexpression of DEPTOR in the medio-basal hypothalamus protects mice against high-fat diet-induced
obesity by modulating
Akt/PKB signaling.[11]
Clinical cancer
Although in most cancer, mTOR pathway is constitutively activated and the expression of DEPTOR is low, one study has found that DEPTOR is overexpressed in
multiple myeloma cells and is necessary for their survival.[7]
Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4.
doi:
10.1016/0378-1119(94)90802-8.
PMID8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56.
doi:
10.1016/S0378-1119(97)00411-3.
PMID9373149.
DEP domain-containing mTOR-interacting protein (DEPTOR) also known as DEP domain-containing protein 6 (DEPDC6) is a
protein that in humans is encoded by the DEPTORgene.[5]
Structure
The gene DEPTOR can be found only in vertebrates. In human, DEPTOR gene locates at chromosome 8, 8q24.12 with protein size 409 a.a.[6] Human DEPTOR contains two
N-terminalDEP domains and a
C-terminalPDZ domain.[7]
Function
DEPTOR is involved in
mTOR signaling pathway as an endogenous regulator. A direct interaction between DEPTOR and mTOR has been shown. [7] Overexpression of DEPTOR downregulates the activity of
mTORC1 and
mTORC2in vitro. mTORC1 and mTORC2 can both inhibit DEPTOR through
phosphorylation.[7]
Metabolism
DEPTOR cell-autonomously regulates
adipogenesis.[8] In the muscle, Baf60c promotes a switch from oxidative to glycolytic myofiber type through DEPTOR-mediated
Akt/PKB activation.[9] Within the brain, DEPTOR is highly expressed in the
hippocampus, the medio-basal
hypothalamus and the
circumventricular organs.[10] Overexpression of DEPTOR in the medio-basal hypothalamus protects mice against high-fat diet-induced
obesity by modulating
Akt/PKB signaling.[11]
Clinical cancer
Although in most cancer, mTOR pathway is constitutively activated and the expression of DEPTOR is low, one study has found that DEPTOR is overexpressed in
multiple myeloma cells and is necessary for their survival.[7]
Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4.
doi:
10.1016/0378-1119(94)90802-8.
PMID8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56.
doi:
10.1016/S0378-1119(97)00411-3.
PMID9373149.