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Cunninghamella elegans
Scientific classification Edit this classification
Domain: Eukaryota
Kingdom: Fungi
Division: Mucoromycota
Class: Mucoromycetes
Order: Mucorales
Family: Cunninghamellaceae
Genus: Cunninghamella
Species:
C. elegans
Binomial name
Cunninghamella elegans
Lendner (1907) [1]
Synonyms
  • Cunninghamella echinulata var. elegans (Lendner) Lunn & Shipton [2]
  • Cunninghamella elegans var. elegans Lendn. 1905

Cunninghamella elegans is a species of fungus in the genus Cunninghamella found in soil. [3]

It can be grown in Sabouraud dextrose broth, a liquid medium used for cultivation of yeasts and molds from liquid which are normally sterile.

As opposed to C. bertholletiae, it is not a human pathogen, [4] with the exception of two documented patients. [5]

Description

Cunninghamella elegans is a filamentous fungus that produces purely gray colonies. [6]

Electron microscopy studies show that the conidia are covered with spines. [7]

Use as a fungal organism capable of xenobiotics metabolism

Cunninghamella elegans is able to degrade xenobiotics. [8] It has a variety of enzymes of phases I (modification enzymes acting to introduce reactive and polar groups into their substrates) and II (conjugation enzymes) of the xenobiotic metabolism, as do mammals. Cytochrome P450 monooxygenase, aryl sulfotransferase, glutathione S-transferase, UDP-glucuronosyltransferase, UDP-glucosyltransferase activities have been detected in cytosolic or microsomal fractions. [9]

Cytochrome P-450 and cytochrome P-450 reductase in C. elegans are part of the phase I enzymes. They are induced by the corticosteroid cortexolone and by phenanthrene. [10] C. elegans also possesses a lanosterol 14-alpha demethylase, another enzyme in the cytochrome P450 family. [11]

Cunninghamella elegans also possesses a glutathione S-transferase. [12]

Use as a fungal model organism of mammalian drug metabolism

Cunninghamella elegans is a microbial model of mammalian drug metabolism. [13] [14] [15] [16] The use of this fungus could reduce the over-all need for laboratory animals. [17]

Cunninghamella elegans is able to transform the tricyclic antidepressants amitriptyline [18] and doxepin, [19] the tetracyclic antidepressant mirtazapine, [20] the muscle relaxant cyclobenzaprine, [21] the typical antipsychotic chlorpromazine as well as the antihistamine and anticholinergic methdilazine [22] and azatadine. It is also able to transform the antihistamines brompheniramine, chlorpheniramine and pheniramine. [23]

It forms a glucoside with the diuretic furosemide. [16]

The transformation of oral contraceptive mestranol by C. elegans yields two hydroxylated metabolites, 6beta-hydroxymestranol and 6beta,12beta-dihydroxymestranol. [24]

Metabolism of polycyclic aromatic hydrocarbons

The phase I cytochrome P450 enzyme systems of C. elegans has been implicated in the neutralization of numerous polycyclic aromatic hydrocarbons (PAH). [6]

It can degrade molecules such as anthracene, 7-methylbenz[a]anthracene and 7-hydroxymethylbenz[a]anthracene, [25] phenanthrene, [26] acenaphthene, [27] 1- and 2-methylnaphthalene, [28] naphthalene, [29] fluorene [30] or benzo(a)pyrene. [31]

In the case of phenanthrene, C. elegans produces a glucoside conjugate of 1-hydroxyphenanthrene ( phenanthrene 1-O-beta-glucose). [32]

Metabolism of pesticides

Cunninghamella elegans is also able to degrade the herbicides alachlor, [33] metolachlor [34] and isoproturon [35] as well as the fungicide mepanipyrim. [3]

Metabolism of phenolics

Cunninghamella elegans can be used to study the metabolism of phenols. This type of molecules already have reactive and polar groups comprised within their structure therefore phases I enzymes are less active than phase II (conjugation) enzymes.

Metabolism of flavonoids

Flavonols

In flavonols, an hydroxyl group is available in the 3- position allowing the glycosylation at that position. The biotransformation of quercetin yields three metabolites, including quercetin 3-O-β-D-glucopyranoside, kaempferol 3-O-β-D-glucopyranoside and isorhamnetin 3-O-β-D-glucopyranoside. Glucosylation and O-methylation are involved in the process. [36]

Flavones

In flavones, there is no hydroxyl group available at the 3- position. Conjugation, in the form of sulfation occurs at the 7- or 4'- positions. Apigenin and chrysin are also transformed by C. elegans and produce apigenin 7-sulfate, apigenin 7,4′-disulfate, chrysin 7-sulfate. [37]
Sulfation also occurs on naringenin and produces naringenin-7-sulfate. [38]

Glucosylation may nevertheless occur but in 3'- position, as happens during the microbial transformation of psiadiarabin and its 6-desmethoxy analogue, 5,3′ dihydroxy-7,2′,4′,5′-tetramethoxyflavone, by Cunninghamella elegans NRRL 1392 that gives the 3′-glucoside conjugates of the two flavones. [39]

flavanones

As in flavones, there is no hydroxyl groups available at the 3- position for glycosylation in flavanones. Therefore, sulfation occurs at the 7- position. In compounds like 7-methoxylated flavanones like 7-O-methylnaringenin ( sakuranetin), demethylation followed by sulfation occur. [40]

Metabolism of synthetic phenolics

It is also able to degrade synthetic phenolic compounds like bisphenol A. [41]

Metabolism of heterocyclic organic compounds

Cunninghamella elegans can transform the nitrogen containing compound phthalazine [42] It is also able to oxidize the organosulfur compound dibenzothiophene. [43]

Uses in biotechnology

Methods for efficient C. elegans genomic DNA isolation and transformation have been developed. [44]

The cytochrome P450 of C. elegans has been cloned in Escherichia coli [45] as well as an enolase. [46]

Use in bioconversion

Techniques employed

Cunninghamella elegans can be grown in stirred tank batch bioreactor. [47] Protoplasts cultures have been used. [48]

Examples of uses

Cunninghamella elegans can be used for phenanthrene bioconversion [47] or for steroid transformation. [48] It has been used to produce isoapocodeine from 10,11-dimethoxyaporphine, [49] triptoquinone from the synthetic abietane diterpene triptophenolide [50] or for the rational and economical bioconversion of antimalarial drug artemisinin to 7beta-hydroxyartemisinin. [51]

Environmental biotechnology

Cunninghamella elegans has been used in environmental biotechnology for the treatment of textile wastewaters, [52] for instance those discoloured by azo dyes [53] or malachite green. [54]

Chitin [55] and chitosan isolated from C. elegans can be used for heavy metal biosorption. [56] Production can be made on yam bean ( Pachyrhizus erosus L. Urban) medium. [57]

Strains

Cunninghamella elegans ATCC 9245 [36]
Cunninghamella elegans ATCC 36112 [6]
Cunninghamella elegans ATCC 26269 [6]
Cunninghamella elegans NRRL 1393 [6]
Cunninghamella elegans IFM 46109 [56]
Cunninghamella elegans UCP 542 [53]

References

  1. ^ Lendner A. (1907). "Sur quelques Mucorinées". Bulletin de l'Herbier Boissier (in French). 7 (3): 249–51.
  2. ^ Weitzmann I. (1984). "The case for Cunninghamella elegans, C. bertholletiae and C. echinulata as separate species". Transactions of the British Mycological Society. 83 (3): 527–529. doi: 10.1016/S0007-1536(84)80056-X.
  3. ^ a b Zhu, Y. Z.; Keum, Y. S.; Yang, L.; Lee, H.; Park, H.; Kim, J. H. (2010). "Metabolism of a Fungicide Mepanipyrim by Soil FungusCunninghamella elegansATCC36112". Journal of Agricultural and Food Chemistry. 58 (23): 12379–12384. doi: 10.1021/jf102980y. PMID  21047134.
  4. ^ Weitzman, I.; Crist, M. Y. (1979). "Studies with clinical isolates of Cunninghamella. I. Mating behavior". Mycologia. 71 (5): 1024–1033. doi: 10.2307/3759290. JSTOR  3759290. PMID  545137.
  5. ^ Kwon-Chung, K. J.; Young, R. C.; Orlando, M. (1975). "Pulmonary mucormycosis caused by Cunninghamella elegans in a patient with chronic myelogenous leukemia". American Journal of Clinical Pathology. 64 (4): 544–548. doi: 10.1093/ajcp/64.4.544. PMID  1060379.
  6. ^ a b c d e Asha S, Vidyavathi M (2009). "Cunninghamella - a microbial model for drug metabolism studies - a review". Biotechnol. Adv. 27 (1): 16–29. doi: 10.1016/j.biotechadv.2008.07.005. PMID  18775773.
  7. ^ Hawker, L. E.; Thomas, B.; Beckett, A. (1970). "An Electron Microscope Study of Structure and Germination of Conidia of Cunninghamella elegans Lendner". Microbiology. 60 (2): 181–189. doi: 10.1099/00221287-60-2-181.
  8. ^ Wackett, L. P.; Gibson, D. T. (1982). "Metabolism of xenobiotic compounds by enzymes in cell extracts of the fungus Cunninghamella elegans". The Biochemical Journal. 205 (1): 117–122. doi: 10.1042/bj2050117. PMC  1158453. PMID  6812568.
  9. ^ Zhang, D.; Yang, Y.; Leakey, J. E. A.; Cerniglia, C. E. (1996). "Phase I and phase II enzymes produced byCunninghamella elegansfor the metabolism of xenobiotics". FEMS Microbiology Letters. 138 (2–3): 221–226. doi: 10.1111/j.1574-6968.1996.tb08161.x. PMID  9026450.
  10. ^ Lisowska, K.; Szemraj, J.; Rózalska, S.; Długoński, J. (2006). "The expression of cytochrome P-450 and cytochrome P-450 reductase genes in the simultaneous transformation of corticosteroids and phenanthrene byCunninghamella elegans". FEMS Microbiology Letters. 261 (2): 175–180. doi: 10.1111/j.1574-6968.2006.00339.x. PMID  16907717.
  11. ^ Lanosterol 14-alpha demethylase from Cunninghamella elegans on www.uniprot.org
  12. ^ Cha, C. J.; Kim, S. J.; Kim, Y. H.; Stingley, R.; Cerniglia, C. E. (2002). "Molecular cloning, expression and characterization of a novel class glutathione S-transferase from the fungus Cunninghamella elegans". Biochemical Journal. 368 (2): 589–95. doi: 10.1042/BJ20020400. PMC  1223007. PMID  12196209.
  13. ^ Kristian Björnstad; Anders Helander; Peter Hultén; Olof Beck (2009). "Bioanalytical investigation of asarone in connection with Acorus calamus oil intoxications". Journal of Analytical Toxicology. 33 (9): 604–609. doi: 10.1093/jat/33.9.604. PMID  20040135.
  14. ^ Joanna D. Moody; Donglu Zhang; Thomas M. Heinze; Carl E. Cerniglia (2000). "Transformation of amoxapine by Cunninghamella elegans". Applied and Environmental Microbiology. 66 (8): 3646–3649. Bibcode: 2000ApEnM..66.3646M. doi: 10.1128/AEM.66.8.3646-3649.2000. PMC  92200. PMID  10919836.
  15. ^ A. Jaworski; L. Sedlaczek; J. Dlugoński; Ewa Zajaczkowska (1985). "Inducible nature of the steroid 11-hydroxylases in spores of Cunninghamella elegans (Lendner)". Journal of Basic Microbiology. 25 (7): 423–427. doi: 10.1002/jobm.3620250703. S2CID  85744435.
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  17. ^ Sharma, KK; Mehta, T; Joshi, V; Mehta, N; Rathor, AK; Mediratta, KD; Sharma, PK (2011). "Substitute of animals in drug research: An approach towards fulfillment of 4R′s". Indian Journal of Pharmaceutical Sciences. 73 (1): 1–6. doi: 10.4103/0250-474X.89750. PMC  3224398. PMID  22131615.
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  19. ^ Moody, J. D.; Freeman, J. P.; Cerniglia, C. E. (1999). "Biotransformation of doxepin by Cunninghamella elegans". Drug Metabolism and Disposition. 27 (10): 1157–1164. PMID  10497142.
  20. ^ Moody, J. D.; Freeman, J. P.; Fu, P. P.; Cerniglia, C. E. (2002). "Biotransformation of Mirtazapine by Cunninghamella Elegans". Drug Metabolism and Disposition. 30 (11): 1274–1279. doi: 10.1124/dmd.30.11.1274. PMID  12386135. S2CID  8257428.
  21. ^ Zhang, D.; Evans, F. E.; Freeman, J. P.; Yang, Y.; Deck, J.; Cerniglia, C. E. (1996). "Formation of mammalian metabolites of cyclobenzaprine by the fungus, Cunninghamella elegans". Chemico-Biological Interactions. 102 (2): 79–92. doi: 10.1016/S0009-2797(96)03736-2. PMID  8950223.
  22. ^ Zhang, D.; Freeman, J. P.; Sutherland, J. B.; Walker, A. E.; Yang, Y.; Cerniglia, C. E. (1996). "Biotransformation of chlorpromazine and methdilazine by Cunninghamella elegans". Applied and Environmental Microbiology. 62 (3): 798–803. Bibcode: 1996ApEnM..62..798Z. doi: 10.1128/AEM.62.3.798-803.1996. PMC  167846. PMID  8975609.
  23. ^ Hansen, E. B.; Cho, B. P.; Korfmacher, W. A.; Cerniglia, C. E. (1995). "Fungal transformations of antihistamines: Metabolism of brompheniramine, chlorpheniramine, and pheniramine to N-oxide and N-demethylated metabolites by the fungus Cunninghamella elegans". Xenobiotica. 25 (10): 1081–1092. doi: 10.3109/00498259509061908. PMID  8578764.
  24. ^ Choudhary, M. I.; Musharraf, S. G.; Siddiqui, Z. A.; Khan, N. T.; Ali, R. A.; Ur-Rahman, A. (2005). "Microbial transformation of mestranol by Cunninghamella elegans". Chemical & Pharmaceutical Bulletin. 53 (8): 1011–1013. doi: 10.1248/cpb.53.1011. PMID  16079537.
  25. ^ Cerniglia, C. E.; Fu, P. P.; Yang, S. K. (1982). "Metabolism of 7-methylbenzaanthracene and 7-hydroxymethylbenzaanthracene by Cunninghamella elegans". Applied and Environmental Microbiology. 44 (3): 682–689. doi: 10.1128/AEM.44.3.682-689.1982. PMC  242076. PMID  7138006.
  26. ^ Cerniglia, C. E.; Yang, S. K. (1984). "Stereoselective metabolism of anthracene and phenanthrene by the fungus Cunninghamella elegans". Applied and Environmental Microbiology. 47 (1): 119–124. Bibcode: 1984ApEnM..47..119C. doi: 10.1128/AEM.47.1.119-124.1984. PMC  239622. PMID  6696409.
  27. ^ Pothuluri, J. V.; Freeman, J. P.; Evans, F. E.; Cerniglia, C. E. (1992). "Fungal metabolism of acenaphthene by Cunninghamella elegans". Applied and Environmental Microbiology. 58 (11): 3654–3659. Bibcode: 1992ApEnM..58.3654P. doi: 10.1128/AEM.58.11.3654-3659.1992. PMC  183157. PMID  1482186.
  28. ^ Cerniglia, C. E.; Lambert, K. J.; Miller, D. W.; Freeman, J. P. (1984). "Transformation of 1- and 2-methylnaphthalene by Cunninghamella elegans". Applied and Environmental Microbiology. 47 (1): 111–118. Bibcode: 1984ApEnM..47..111C. doi: 10.1128/AEM.47.1.111-118.1984. PMC  239621. PMID  6696408.
  29. ^ Cerniglia, C. E.; Gibson, D. T. (1977). "Metabolism of naphthalene by Cunninghamella elegans". Applied and Environmental Microbiology. 34 (4): 363–370. Bibcode: 1977ApEnM..34..363C. doi: 10.1128/AEM.34.4.363-370.1977. PMC  242664. PMID  921262.
  30. ^ Pothuluri, J. V.; Freeman, J. P.; Evans, F. E.; Cerniglia, C. E. (1993). "Biotransformation of fluorene by the fungus Cunninghamella elegans". Applied and Environmental Microbiology. 59 (6): 1977–1980. Bibcode: 1993ApEnM..59.1977P. doi: 10.1128/AEM.59.6.1977-1980.1993. PMC  182201. PMID  8328814.
  31. ^ Cerniglia, C. E.; Mahaffey, W.; Gibson, D. T. (1980). "Fungal oxidation of benzo\a]pyrene: Formation of (−)-trans-7,8-dihydroxy-7,8-dihydrobenzo\a]pyrene by Cunninghamella elegans". Biochemical and Biophysical Research Communications. 94 (1): 226–232. doi: 10.1016/S0006-291X(80)80210-5. PMID  7190014.
  32. ^ Cerniglia, C. E.; Campbell, W. L.; Freeman, J. P.; Evans, F. E. (1989). "Identification of a novel metabolite in phenanthrene metabolism by the fungus Cunninghamella elegans". Applied and Environmental Microbiology. 55 (9): 2275–2279. Bibcode: 1989ApEnM..55.2275C. doi: 10.1128/AEM.55.9.2275-2279.1989. PMC  203068. PMID  2802607.
  33. ^ Pothuluri, J. V.; Freeman, J. P.; Evans, F. E.; Moorman, T. B.; Cerniglia, C. E. (1993). "Metabolism of alachlor by the fungus Cunninghamella elegans". Journal of Agricultural and Food Chemistry. 41 (3): 483–488. doi: 10.1021/jf00027a026.
  34. ^ Jairaj V. Pothuluri, Frederick E. Evans; Doerge, D.R.; Churchwell, M.I. & Carl E. Cerniglia (1997). "Metabolism of metolachlor by the fungus Cunninghamella elegans". Arch. Environ. Contam. Toxicol. 32 (2): 117–125. doi: 10.1007/s002449900163. PMID  9069185. S2CID  20614148.
  35. ^ Hangler, M.; Jensen, B.; Rønhede, S.; Sørensen, S. R. (2007). "Inducible hydroxylation and demethylation of the herbicide isoproturon by Cunninghamella elegans". FEMS Microbiology Letters. 268 (2): 254–260. doi: 10.1111/j.1574-6968.2006.00599.x. PMID  17328751.
  36. ^ a b Zi, J.; Valiente, J.; Zeng, J.; Zhan, J. (2011). "Metabolism of quercetin by Cunninghamella elegans ATCC 9245". Journal of Bioscience and Bioengineering. 112 (4): 360–362. doi: 10.1016/j.jbiosc.2011.06.006. PMID  21742550.
  37. ^ Ibrahim, A. R. S. (2005). "Biotransformation of Chrysin and Apigenin by Cunninghamella elegans". Chemical & Pharmaceutical Bulletin. 53 (6): 671–672. doi: 10.1248/cpb.53.671. PMID  15930780.
  38. ^ Abdel-Rahim S. Ibrahim (2000). "Sulfation of naringenin by Cunninghamella elegans". Phytochemistry. 53 (2): 209–212. Bibcode: 2000PChem..53..209I. doi: 10.1016/S0031-9422(99)00487-2. PMID  10680173.
  39. ^ Ibrahim, A. R.; Galal, A. M.; Mossa, J. S.; El-Feraly, F. S. (1997). "Glucose-conjugation of the flavones of Psiadia arabica by Cunninghamella elegans". Phytochemistry. 46 (7): 1193–1195. doi: 10.1016/s0031-9422(97)00421-4. PMID  9423290.
  40. ^ Ibrahim, A. R.; Galal, A. M.; Ahmed, M. S.; Mossa, G. S. (2003). "O-demethylation and sulfation of 7-methoxylated flavanones by Cunninghamella elegans". Chemical & Pharmaceutical Bulletin. 51 (2): 203–206. doi: 10.1248/cpb.51.203. PMID  12576658. INIST  14569933.
  41. ^ Keum, Y. S.; Lee, H. R.; Park, H. W.; Kim, J. H. (2010). "Biodegradation of bisphenol a and its halogenated analogues by Cunninghamella elegans ATCC36112". Biodegradation. 21 (6): 989–997. doi: 10.1007/s10532-010-9358-8. PMID  20455075. S2CID  2259930.
  42. ^ Sutherland, John B.; Freeman, James P.; Williams, Anna J.; Deck, Joanna (1999). "Biotransformation of Phthalazine by Fusarium moniliforme and Cunninghamela elegans". Mycologia. 91 (1): 114–116. doi: 10.2307/3761198. JSTOR  3761198.
  43. ^ Crawford, D. L.; Gupta, R. K. (1990). "Oxidation of dibenzothiophene byCunninghamella elegans". Current Microbiology. 21 (4): 229–231. doi: 10.1007/BF02092161. S2CID  6892038.
  44. ^ Zhang, D.; Yang, Y.; Castlebury, L. A.; Cerniglia, C. E. (1996). "A method for the large scale isolation of high transformation efficiency fungal genomic DNA". FEMS Microbiology Letters. 145 (2): 261–265. doi: 10.1111/j.1574-6968.1996.tb08587.x. PMID  8961565.
  45. ^ Wang, R. F.; Cao, W. W.; Khan, A. A.; Cerniglia, C. E. (2000). "Cloning, sequencing, and expression inEscherichia coliof a cytochrome P450 gene from Cunninghamella elegans". FEMS Microbiology Letters. 188 (1): 55–61. doi: 10.1111/j.1574-6968.2000.tb09168.x. PMID  10867234.
  46. ^ Wang, R. F.; Khan, A. A.; Cao, W. W.; Cerniglia, C. E. (2000). "Cloning, sequencing, and expression of the gene encoding enolase from Cunninghamella elegans". Mycological Research. 104 (2): 175–179. doi: 10.1017/S0953756299001112.
  47. ^ a b Lisowska, K.; Bizukojc, M.; Długoński, J. (2006). "An unstructured model for studies on phenanthrene bioconversion by filamentous fungus Cunninghamella elegans". Enzyme and Microbial Technology. 39 (7): 1464–1470. doi: 10.1016/j.enzmictec.2006.03.039.
  48. ^ a b Długoński, J.; Sedlaczek, L.; Jaworski, A. (1984). "Protoplast release from fungi capable of steroid transformation". Canadian Journal of Microbiology. 30 (1): 57–62. doi: 10.1139/m84-010. PMID  6713303. (French)
  49. ^ Smith, R. V.; Davis, P. J. (1978). "Regiospecific synthesis of isoapocodeine from 10,11-dimethoxyaporphine by using Cunninghamella elegans". Applied and Environmental Microbiology. 35 (4): 738–742. Bibcode: 1978ApEnM..35..738S. doi: 10.1128/AEM.35.4.738-742.1978. PMC  242915. PMID  25623.
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  51. ^ Parshikov, I. A.; Muraleedharan, K. M.; Avery, M. A.; Williamson, J. S. (2004). "Transformation of artemisinin by Cunninghamella elegans". Applied Microbiology and Biotechnology. 64 (6): 782–786. doi: 10.1007/s00253-003-1524-z. PMID  14735322. S2CID  6542162.
  52. ^ Tigini, V.; Prigione, V.; Donelli, I.; Anastasi, A.; Freddi, G.; Giansanti, P.; Mangiavillano, A.; Varese, G. C. (2010). "Cunninghamella elegans biomass optimisation for textile wastewater biosorption treatment: An analytical and ecotoxicological approach". Applied Microbiology and Biotechnology. 90 (1): 343–352. doi: 10.1007/s00253-010-3010-8. hdl: 2318/82517. PMID  21127858. S2CID  23402412.
  53. ^ a b Ambrósio, S.; Campos-Takaki, G. M. (2004). "Decolorization of reactive azo dyes by Cunninghamella elegans UCP 542 under co-metabolic conditions". Bioresource Technology. 91 (1): 69–75. doi: 10.1016/S0960-8524(03)00153-6. PMID  14585623.
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External links

Retrieved from " https://en.wikipedia.org/?title=Cunninghamella_elegans&oldid=1194824730"
From Wikipedia, the free encyclopedia

Cunninghamella elegans
Scientific classification Edit this classification
Domain: Eukaryota
Kingdom: Fungi
Division: Mucoromycota
Class: Mucoromycetes
Order: Mucorales
Family: Cunninghamellaceae
Genus: Cunninghamella
Species:
C. elegans
Binomial name
Cunninghamella elegans
Lendner (1907) [1]
Synonyms
  • Cunninghamella echinulata var. elegans (Lendner) Lunn & Shipton [2]
  • Cunninghamella elegans var. elegans Lendn. 1905

Cunninghamella elegans is a species of fungus in the genus Cunninghamella found in soil. [3]

It can be grown in Sabouraud dextrose broth, a liquid medium used for cultivation of yeasts and molds from liquid which are normally sterile.

As opposed to C. bertholletiae, it is not a human pathogen, [4] with the exception of two documented patients. [5]

Description

Cunninghamella elegans is a filamentous fungus that produces purely gray colonies. [6]

Electron microscopy studies show that the conidia are covered with spines. [7]

Use as a fungal organism capable of xenobiotics metabolism

Cunninghamella elegans is able to degrade xenobiotics. [8] It has a variety of enzymes of phases I (modification enzymes acting to introduce reactive and polar groups into their substrates) and II (conjugation enzymes) of the xenobiotic metabolism, as do mammals. Cytochrome P450 monooxygenase, aryl sulfotransferase, glutathione S-transferase, UDP-glucuronosyltransferase, UDP-glucosyltransferase activities have been detected in cytosolic or microsomal fractions. [9]

Cytochrome P-450 and cytochrome P-450 reductase in C. elegans are part of the phase I enzymes. They are induced by the corticosteroid cortexolone and by phenanthrene. [10] C. elegans also possesses a lanosterol 14-alpha demethylase, another enzyme in the cytochrome P450 family. [11]

Cunninghamella elegans also possesses a glutathione S-transferase. [12]

Use as a fungal model organism of mammalian drug metabolism

Cunninghamella elegans is a microbial model of mammalian drug metabolism. [13] [14] [15] [16] The use of this fungus could reduce the over-all need for laboratory animals. [17]

Cunninghamella elegans is able to transform the tricyclic antidepressants amitriptyline [18] and doxepin, [19] the tetracyclic antidepressant mirtazapine, [20] the muscle relaxant cyclobenzaprine, [21] the typical antipsychotic chlorpromazine as well as the antihistamine and anticholinergic methdilazine [22] and azatadine. It is also able to transform the antihistamines brompheniramine, chlorpheniramine and pheniramine. [23]

It forms a glucoside with the diuretic furosemide. [16]

The transformation of oral contraceptive mestranol by C. elegans yields two hydroxylated metabolites, 6beta-hydroxymestranol and 6beta,12beta-dihydroxymestranol. [24]

Metabolism of polycyclic aromatic hydrocarbons

The phase I cytochrome P450 enzyme systems of C. elegans has been implicated in the neutralization of numerous polycyclic aromatic hydrocarbons (PAH). [6]

It can degrade molecules such as anthracene, 7-methylbenz[a]anthracene and 7-hydroxymethylbenz[a]anthracene, [25] phenanthrene, [26] acenaphthene, [27] 1- and 2-methylnaphthalene, [28] naphthalene, [29] fluorene [30] or benzo(a)pyrene. [31]

In the case of phenanthrene, C. elegans produces a glucoside conjugate of 1-hydroxyphenanthrene ( phenanthrene 1-O-beta-glucose). [32]

Metabolism of pesticides

Cunninghamella elegans is also able to degrade the herbicides alachlor, [33] metolachlor [34] and isoproturon [35] as well as the fungicide mepanipyrim. [3]

Metabolism of phenolics

Cunninghamella elegans can be used to study the metabolism of phenols. This type of molecules already have reactive and polar groups comprised within their structure therefore phases I enzymes are less active than phase II (conjugation) enzymes.

Metabolism of flavonoids

Flavonols

In flavonols, an hydroxyl group is available in the 3- position allowing the glycosylation at that position. The biotransformation of quercetin yields three metabolites, including quercetin 3-O-β-D-glucopyranoside, kaempferol 3-O-β-D-glucopyranoside and isorhamnetin 3-O-β-D-glucopyranoside. Glucosylation and O-methylation are involved in the process. [36]

Flavones

In flavones, there is no hydroxyl group available at the 3- position. Conjugation, in the form of sulfation occurs at the 7- or 4'- positions. Apigenin and chrysin are also transformed by C. elegans and produce apigenin 7-sulfate, apigenin 7,4′-disulfate, chrysin 7-sulfate. [37]
Sulfation also occurs on naringenin and produces naringenin-7-sulfate. [38]

Glucosylation may nevertheless occur but in 3'- position, as happens during the microbial transformation of psiadiarabin and its 6-desmethoxy analogue, 5,3′ dihydroxy-7,2′,4′,5′-tetramethoxyflavone, by Cunninghamella elegans NRRL 1392 that gives the 3′-glucoside conjugates of the two flavones. [39]

flavanones

As in flavones, there is no hydroxyl groups available at the 3- position for glycosylation in flavanones. Therefore, sulfation occurs at the 7- position. In compounds like 7-methoxylated flavanones like 7-O-methylnaringenin ( sakuranetin), demethylation followed by sulfation occur. [40]

Metabolism of synthetic phenolics

It is also able to degrade synthetic phenolic compounds like bisphenol A. [41]

Metabolism of heterocyclic organic compounds

Cunninghamella elegans can transform the nitrogen containing compound phthalazine [42] It is also able to oxidize the organosulfur compound dibenzothiophene. [43]

Uses in biotechnology

Methods for efficient C. elegans genomic DNA isolation and transformation have been developed. [44]

The cytochrome P450 of C. elegans has been cloned in Escherichia coli [45] as well as an enolase. [46]

Use in bioconversion

Techniques employed

Cunninghamella elegans can be grown in stirred tank batch bioreactor. [47] Protoplasts cultures have been used. [48]

Examples of uses

Cunninghamella elegans can be used for phenanthrene bioconversion [47] or for steroid transformation. [48] It has been used to produce isoapocodeine from 10,11-dimethoxyaporphine, [49] triptoquinone from the synthetic abietane diterpene triptophenolide [50] or for the rational and economical bioconversion of antimalarial drug artemisinin to 7beta-hydroxyartemisinin. [51]

Environmental biotechnology

Cunninghamella elegans has been used in environmental biotechnology for the treatment of textile wastewaters, [52] for instance those discoloured by azo dyes [53] or malachite green. [54]

Chitin [55] and chitosan isolated from C. elegans can be used for heavy metal biosorption. [56] Production can be made on yam bean ( Pachyrhizus erosus L. Urban) medium. [57]

Strains

Cunninghamella elegans ATCC 9245 [36]
Cunninghamella elegans ATCC 36112 [6]
Cunninghamella elegans ATCC 26269 [6]
Cunninghamella elegans NRRL 1393 [6]
Cunninghamella elegans IFM 46109 [56]
Cunninghamella elegans UCP 542 [53]

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