| CRISPR-associated protein 4 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
Pyrobaculum calidifontis Cas4,
PDB:
4R5Q | |||||||||
| Identifiers | |||||||||
| Symbol | cas4 | ||||||||
| Pfam | PF01930 | ||||||||
| InterPro | IPR022765 | ||||||||
| |||||||||
Cas4 is an endonuclease found in some CRISPR systems that is used as part of the spacer acquisition stage.
Cas4 is required for efficient prespacer processing by forming a Cas4- Cas1- Cas2 complex. [1] In this complex, Cas4 cuts double-stranded substrates with long 3'-overhangs through site-specific endonucleolytic cleavage. [2] Cas4 closely interacts with the integrase active sites of Cas1, recognizes PAM sequences within the substrate and cleaves precisely upstream of them, ensuring the acquisition of functional spacers. [2] [1]
References
- ^ a b Lee H, Dhingra Y, Sashital DG (April 2019). "The Cas4-Cas1-Cas2 complex mediates precise prespacer processing during CRISPR adaptation". eLife. 8. doi: 10.7554/eLife.44248. PMC 6519985. PMID 31021314.
- ^ a b Lee H, Zhou Y, Taylor DW, Sashital DG (April 2018). "Cas4-Dependent Prespacer Processing Ensures High-Fidelity Programming of CRISPR Arrays". Molecular Cell. 70 (1): 48–59.e5. doi: 10.1016/j.molcel.2018.03.003. PMC 5889325. PMID 29602742.
 | This enzyme-related article is a stub. You can help Wikipedia by expanding it. |
| CRISPR-associated protein 4 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
|
Pyrobaculum calidifontis Cas4,
PDB:
4R5Q | |||||||||
| Identifiers | |||||||||
| Symbol | cas4 | ||||||||
| Pfam | PF01930 | ||||||||
| InterPro | IPR022765 | ||||||||
| |||||||||
Cas4 is an endonuclease found in some CRISPR systems that is used as part of the spacer acquisition stage.
Cas4 is required for efficient prespacer processing by forming a Cas4- Cas1- Cas2 complex. [1] In this complex, Cas4 cuts double-stranded substrates with long 3'-overhangs through site-specific endonucleolytic cleavage. [2] Cas4 closely interacts with the integrase active sites of Cas1, recognizes PAM sequences within the substrate and cleaves precisely upstream of them, ensuring the acquisition of functional spacers. [2] [1]
References
- ^ a b Lee H, Dhingra Y, Sashital DG (April 2019). "The Cas4-Cas1-Cas2 complex mediates precise prespacer processing during CRISPR adaptation". eLife. 8. doi: 10.7554/eLife.44248. PMC 6519985. PMID 31021314.
- ^ a b Lee H, Zhou Y, Taylor DW, Sashital DG (April 2018). "Cas4-Dependent Prespacer Processing Ensures High-Fidelity Programming of CRISPR Arrays". Molecular Cell. 70 (1): 48–59.e5. doi: 10.1016/j.molcel.2018.03.003. PMC 5889325. PMID 29602742.
|
| This enzyme-related article is a stub. You can help Wikipedia by expanding it. |