Cleavage and polyadenylation specificity factor subunit 4 is a
protein that in humans is encoded by the CPSF4gene.[5][6][7]
Inhibition of the nuclear export of poly(A)-containing
mRNAs caused by the
influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and
polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs.
In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents
CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs.
Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different
isoforms have been described for this gene.[7]
Overview of all the structural information available in the
PDB for
UniProt: O95639 (Cleavage and polyadenylation specificity factor subunit 4) at the
PDBe-KB.
Cleavage and polyadenylation specificity factor subunit 4 is a
protein that in humans is encoded by the CPSF4gene.[5][6][7]
Inhibition of the nuclear export of poly(A)-containing
mRNAs caused by the
influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and
polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs.
In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents
CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs.
Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different
isoforms have been described for this gene.[7]
Overview of all the structural information available in the
PDB for
UniProt: O95639 (Cleavage and polyadenylation specificity factor subunit 4) at the
PDBe-KB.