Baby Hamster Kidney fibroblasts (BHK cells) are an adherent cell line used in molecular biology. The cells were derived in 1961 by I. A. Macpherson and M. G. P. Stoker. Nowadays, subclone 13 is occasionally used, which was originally derived by single-cell isolation from the kidneys of five unsexed, 1-day-old hamsters.
BHK and virus infection
BHK-21 cells are susceptible to human adenovirus D, reovirus 3, and vesicular stomatitis virus (Indiana strain). [1] BHK-21 cells are resistant to poliovirus 2 and Rabbit vesivirus (RaV). The cells are negative for reverse transcriptase, which means that they lack integral retrovirus genomes. [2]
Utilization
The BHK-21 cells are useful for transformations and for stable and temporary transfections. [2] BHK cells are also used to study viral infections. [1] [3]
Recommended growth medium
- High-glucose DMEM
- FBS fetal bovine serum: 5% (15% when freeze)
- GLU glutamine: 1%
- PSA regular antibiotics: 1%
- Splitting: 10 by trypsin [4]
References
- ^ a b Timm C, Gupta A, Yin J (August 2015). "Robust kinetics of an RNA virus: Transcription rates are set by genome levels". Biotechnology and Bioengineering. 112 (8): 1655–62. doi: 10.1002/bit.25578. PMC 5653219. PMID 25726926.
- ^ a b "BHK-21 Cells". Retrieved 24 April 2018.
- ^ Lim KI, Lang T, Lam V, Yin J (September 2006). "Model-based design of growth-attenuated viruses". PLOS Computational Biology. 2 (9): e116. Bibcode: 2006PLSCB...2..116L. doi: 10.1371/journal.pcbi.0020116. PMC 1557587. PMID 16948530.
- ^ "Microscopy Resource Center | Olympus Life Science". www.olympusmicro.com. Archived from the original on 2009-12-27. Retrieved 2009-10-31.
External links
Baby Hamster Kidney fibroblasts (BHK cells) are an adherent cell line used in molecular biology. The cells were derived in 1961 by I. A. Macpherson and M. G. P. Stoker. Nowadays, subclone 13 is occasionally used, which was originally derived by single-cell isolation from the kidneys of five unsexed, 1-day-old hamsters.
BHK and virus infection
BHK-21 cells are susceptible to human adenovirus D, reovirus 3, and vesicular stomatitis virus (Indiana strain). [1] BHK-21 cells are resistant to poliovirus 2 and Rabbit vesivirus (RaV). The cells are negative for reverse transcriptase, which means that they lack integral retrovirus genomes. [2]
Utilization
The BHK-21 cells are useful for transformations and for stable and temporary transfections. [2] BHK cells are also used to study viral infections. [1] [3]
Recommended growth medium
- High-glucose DMEM
- FBS fetal bovine serum: 5% (15% when freeze)
- GLU glutamine: 1%
- PSA regular antibiotics: 1%
- Splitting: 10 by trypsin [4]
References
- ^ a b Timm C, Gupta A, Yin J (August 2015). "Robust kinetics of an RNA virus: Transcription rates are set by genome levels". Biotechnology and Bioengineering. 112 (8): 1655–62. doi: 10.1002/bit.25578. PMC 5653219. PMID 25726926.
- ^ a b "BHK-21 Cells". Retrieved 24 April 2018.
- ^ Lim KI, Lang T, Lam V, Yin J (September 2006). "Model-based design of growth-attenuated viruses". PLOS Computational Biology. 2 (9): e116. Bibcode: 2006PLSCB...2..116L. doi: 10.1371/journal.pcbi.0020116. PMC 1557587. PMID 16948530.
- ^ "Microscopy Resource Center | Olympus Life Science". www.olympusmicro.com. Archived from the original on 2009-12-27. Retrieved 2009-10-31.