This article needs additional citations for verification. Please help improve this article by adding citations to reliable sources. Unsourced material may be challenged and removed. Find sources:  "All-time Orlando City B roster" –  news · newspapers · books · scholar · JSTOR (December 2018) ( Learn how and when to remove this message)

This list comprises all players who have participated in at least one league match for Orlando City B since the team's first USL season in 2016.

A "†" denotes players who only appeared in a single match.

Accurate as of match played April 24, 2022.

• Neicer Acosta (2022–present)
• Raul Aguilera (2020)
• Gonzalo Agustoni-Chagas (2022–present)
• Kevin Alston (2017)
• Mikey Ambrose (2016)
• Austin Amer (2019–2020)
• Austin Aviza (2020)

• Will Bagrou (2019)
• Hadji Barry (2016–2017)
• Jordan Bender (2019–2020)
• David Boccuzzo (2022–present)
• Luke Boden (2016)
• Mauro Bravo (2022–present)

• Kyle Callan-McFadden (2016)
• Franklin Carabalí (2020)
• Zach Carroll (2017)
• Paul Clowes (2017)
• Michael Cox (2016–2017)
• Isaque Couto (2016)

• Pierre da Silva (2016–2017)
• Danny Deakin (2017)
• Joey DeZart (2020–present)
• Albert Dikwa (2017)
• Ates Diouf (2019)
• Conor Donovan (2016–2017)

• Earl Edwards Jr. (2016–2017)
• Zachary Ellis-Hayden (2016–2017)
• William Eyang (2016)

• Fablo (2020)
• Jake Fenlason (2016–2017)
• Jahlane Forbes (2019–2020)
• Andrew Forth (2022–present)
• Theo Franca (2022–present)
• Alex Freeman (2022–present)

• Joe Gallardo (2017)
• Alejandro García (2016)
• Devron García (2016)
• Aleksandar Gluvačević (2020)
• Alejandro Granados (2022–present)
• Luc Granitur (2019)
• Quembol Guadalupe (2022–present)
• Erick Gunera-Calix (2022–present)
• Liam Guske (2022–present)
• Owen Guske (2020)

• Brandon Hackenberg (2022–present)
• Emmanuel Hagan (2019)
• Landen Haig (2016)
• Michael Halliday (2020–present)
• Harrison Heath (2016)
• Steven Hernandez (2019)
• Christian Herrera (2019)
• Cristian Higuita (2016)
• Jordan Hill (2019)
• Seb Hines (2017)
• Tanner Hummel (2019)

• Sebastian Joffre (2019)
• Brandon John (2019)
• Juliano (2019)

• Julian Kennedy (2020)
• Nabilai Kibunguchy (2023)
• Ryley Kraft (2017)

• Mason Lamb (2020)
• Richie Laryea (2016–2017)
• David Loera (2016–2017)
• Brian Lopez (2022–present)
• Jack Lynn (2022–present)

• Luca Mancuso † (2019)
• Austin Martz (2017)
• Antonio Matarazzo (2016)
• Tresor Mbuyu (2019)
• Jonathan Mendoza (2016)
• Randy Mendoza (2019)
• Juan Pablo Monticelli (2020)

• Theodore Ndje (2020)
• Lewis Neal (2016–2017)
• Craig Nitti (2016)

• Marius Obekop (2016)
• Nick O'Callaghan (2020)
• Lucas Ontivero (2019)
• Koby Osei-Wusu (2019)
• Javier Otero (2020–present)
• Adam Ozeri (2020)

• Diego Pareja (2022–present)
• PC (2017)
• Léo Pereira (2017)
• Ben Polk (2017)

• José Quintero (2019–2020)

• Rafael Ramos (2016–2017)
• Tommy Redding (2016–2017)
• Andrew Ribeiro (2016)
• Pedro Ribeiro (2016)
• Mark Ridgers (2016)
• Wilfredo Rivera (2020–present)
• Tony Rocha (2016–2017)
• Bryan Róchez (2016)
• Mateo Rodas (2020)
• Jonathan Rosales (2020)
• Daniel Rosario (2020)

• Moussa Sane (2017)
• Rafael Santos (2019)
• Josh Saunders (2017)
• Jordan Schweitzer (2017)
• AJ Seals (2017)
• Sérginho (2019)
• Ian Silva (2022–present)
• Matheus Silva (2019)
• Léo Simas (2019)
• Nathan Simeon (2019)
• Keegan Smith (2016)
• Thiago de Souza (2019)
• Mason Stajduhar (2020)

• Moises Tablante (2019–present)
• Kenji Tanaka (2020)
• Nick Taylor (2022–present)
• Scott Thomsen (2017)
• Fernando Timbó (2017)
• Tyler Turner (2016)

• Owen Van Marter (2022–present)

• Thomas Williams (2020–present)
• Wilfred Williams (2019)

• Victor Yan (2022–present)
• Jules Youmeni (2017)