| Acholeplasma laidlawii | |
|---|---|
Scientific classification
| |
| Domain: | Bacteria |
| Phylum: | Mycoplasmatota |
| Class: | Mollicutes |
| Order: | Acholeplasmatales |
| Family: | Acholeplasmataceae |
| Genus: | Acholeplasma |
| Species: | A. laidlawii
|
| Binomial name | |
| Acholeplasma laidlawii (Sabin 1941) Edward and Freundt 1970
[1]
| |
Acholeplasma laidlawii are small bacteria which lack a cell wall. [2] Like other Acholeplasma and Mycoplasma, A. laidlawii has been identified as a common contaminant of growth media for cell culture. [3]
A. laidlawii was first isolated from sewage in London in 1936 and was named after its discoverer, Patrick Laidlaw. [4]
A. laidlawii has a relatively small genome comprising 1.5 Mbp. Additionally its genome has a low GC-content of just 31%. [2] The A. laidlawii genome has been sequenced. [2]
Acholeplasma laidlawii may contaminate bovine serum and also occurs in serum-free cell culture media products. The presence of A. laidlawii in broth powders is a serious problem in routine biopharmaceutical operations where filtration is used as a sterilisation procedure. A. laidlawii may flourish and survive for prolonged periods at refrigeration and ambient temperatures in serum-free cell culture media.
- ^ Parte, A.C. "Acholeplasma". LPSN.
- ^ a b c Lazarev VN; Levitskii SA; Basovskii YL; Chukin MM; Akopian TA; et al. (September 2011). "Complete genome and Proteome of Acholeplasma laidlawii". J. Bacteriol. 193 (18): 4943–4953. doi: 10.1128/jb.05059-11. PMC 3165704. PMID 21784942.
- ^ Windsor HM; Windsor GD; Noordergraaf JH (March 2010). "The growth and long term survival of Acholeplasma laidlawii in media products used in biopharmaceutical manufacturing". Biologicals. 38 (2): 204–210. doi: 10.1016/j.biologicals.2009.11.009. PMID 20153666.
- ^ Laidlaw PP; Elford WJ (2 June 1936). "A New Group of Filterable Organisms". Proceedings of the Royal Society of London. 120 (818): 292–303. doi: 10.1098/rspb.1936.0036. JSTOR 82051.
| Acholeplasma laidlawii | |
|---|---|
|
Scientific classification
| |
| Domain: | Bacteria |
| Phylum: | Mycoplasmatota |
| Class: | Mollicutes |
| Order: | Acholeplasmatales |
| Family: | Acholeplasmataceae |
| Genus: | Acholeplasma |
| Species: | A. laidlawii
|
| Binomial name | |
| Acholeplasma laidlawii (Sabin 1941) Edward and Freundt 1970
[1]
| |
Acholeplasma laidlawii are small bacteria which lack a cell wall. [2] Like other Acholeplasma and Mycoplasma, A. laidlawii has been identified as a common contaminant of growth media for cell culture. [3]
A. laidlawii was first isolated from sewage in London in 1936 and was named after its discoverer, Patrick Laidlaw. [4]
A. laidlawii has a relatively small genome comprising 1.5 Mbp. Additionally its genome has a low GC-content of just 31%. [2] The A. laidlawii genome has been sequenced. [2]
Acholeplasma laidlawii may contaminate bovine serum and also occurs in serum-free cell culture media products. The presence of A. laidlawii in broth powders is a serious problem in routine biopharmaceutical operations where filtration is used as a sterilisation procedure. A. laidlawii may flourish and survive for prolonged periods at refrigeration and ambient temperatures in serum-free cell culture media.
- ^ Parte, A.C. "Acholeplasma". LPSN.
- ^ a b c Lazarev VN; Levitskii SA; Basovskii YL; Chukin MM; Akopian TA; et al. (September 2011). "Complete genome and Proteome of Acholeplasma laidlawii". J. Bacteriol. 193 (18): 4943–4953. doi: 10.1128/jb.05059-11. PMC 3165704. PMID 21784942.
- ^ Windsor HM; Windsor GD; Noordergraaf JH (March 2010). "The growth and long term survival of Acholeplasma laidlawii in media products used in biopharmaceutical manufacturing". Biologicals. 38 (2): 204–210. doi: 10.1016/j.biologicals.2009.11.009. PMID 20153666.
- ^ Laidlaw PP; Elford WJ (2 June 1936). "A New Group of Filterable Organisms". Proceedings of the Royal Society of London. 120 (818): 292–303. doi: 10.1098/rspb.1936.0036. JSTOR 82051.