![]() | |
Names | |
---|---|
IUPAC name
2-Amino-6-methyl-1,8-dihydropteridine-4,7-dione
| |
Other names
6-MI; 6MI
| |
Identifiers | |
3D model (
JSmol)
|
|
ChemSpider | |
ECHA InfoCard | 100.149.651 |
PubChem
CID
|
|
UNII | |
CompTox Dashboard (
EPA)
|
|
| |
| |
Properties | |
C7H7N5O2 | |
Molar mass | 193.166 g·mol−1 |
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
|
6-Methylisoxanthopterin (6MI) is a base analog for the nucleotide guanine. It is useful as a fluorescent indicator because unlike most other base analogs, quenching does not occur when it is incorporated into a double helix. In fact, it exhibits a 3 to 4-fold increase in quantum yield when it is incorporated into a duplex formation. [1] This allows 6MI to be used to probe the dynamics of DNA or RNA helices using a technique such as fluorescence polarization anisotropy. [2]
![]() | |
Names | |
---|---|
IUPAC name
2-Amino-6-methyl-1,8-dihydropteridine-4,7-dione
| |
Other names
6-MI; 6MI
| |
Identifiers | |
3D model (
JSmol)
|
|
ChemSpider | |
ECHA InfoCard | 100.149.651 |
PubChem
CID
|
|
UNII | |
CompTox Dashboard (
EPA)
|
|
| |
| |
Properties | |
C7H7N5O2 | |
Molar mass | 193.166 g·mol−1 |
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
|
6-Methylisoxanthopterin (6MI) is a base analog for the nucleotide guanine. It is useful as a fluorescent indicator because unlike most other base analogs, quenching does not occur when it is incorporated into a double helix. In fact, it exhibits a 3 to 4-fold increase in quantum yield when it is incorporated into a duplex formation. [1] This allows 6MI to be used to probe the dynamics of DNA or RNA helices using a technique such as fluorescence polarization anisotropy. [2]